Carla Luciana Abán, Silvia Orosco, Julio Nicolás Argañaraz Aybar, Leonardo Albarracín, Analía Venecia, Liliana Perret, Sonia Ortiz Mayor, Keita Nishiyama, Juan Carlos Valdéz, Haruki Kitazawa, Julio Villena, Nadia Gobbato
{"title":"Effect of <i>Lactiplantibacillus plantarum</i> cell-free culture on bacterial pathogens isolated from cystic fibrosis patients: <i>in vitro</i> and <i>in vivo</i> studies.","authors":"Carla Luciana Abán, Silvia Orosco, Julio Nicolás Argañaraz Aybar, Leonardo Albarracín, Analía Venecia, Liliana Perret, Sonia Ortiz Mayor, Keita Nishiyama, Juan Carlos Valdéz, Haruki Kitazawa, Julio Villena, Nadia Gobbato","doi":"10.3389/fmicb.2024.1440090","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed to investigate the effects of the cell-free supernatant of <i>Lactiplantibacillus plantarum</i> ATCC<sup>®</sup> 10241<sup>TM</sup> on the biofilm-forming capacity of <i>Pseudomonas aeruginosa</i> strains isolated from cystic fibrosis (CF) patients. In addition, the study evaluated the <i>in vivo</i> potential of the cell-free supernatant to modulate inflammation and reduce lung damage in mice infected with <i>P. aeruginosa</i> strains or co-challenged with <i>P. aeruginosa</i> and the <i>Streptococcus milleri</i> group (SMG). The results showed that CF-derived <i>P. aeruginosa</i> strains can infect the respiratory tract of adult mice, inducing local inflammation and lung damage. The severity of these infections was exacerbated when <i>P. aeruginosa</i> was co-administered with SMG. Notably, nebulization with the cell-free supernatant of <i>L. plantarum</i> produced beneficial effects, reducing respiratory infection severity and inflammatory responses induced by <i>P. aeruginosa</i>, both alone or in combination with SMG. Reduced bacterial loads and lung damage were observed in supernatant-treated mice compared to controls. Although further mechanistic studies are necessary, the results show that the cell-free supernatant of <i>L. plantarum</i> ATCC<sup>®</sup> 10241<sup>TM</sup> is an interesting adjuvant alternative to treat <i>P. aeruginosa</i> respiratory infections and superinfections in CF patients.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11439784/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fmicb.2024.1440090","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This study aimed to investigate the effects of the cell-free supernatant of Lactiplantibacillus plantarum ATCC® 10241TM on the biofilm-forming capacity of Pseudomonas aeruginosa strains isolated from cystic fibrosis (CF) patients. In addition, the study evaluated the in vivo potential of the cell-free supernatant to modulate inflammation and reduce lung damage in mice infected with P. aeruginosa strains or co-challenged with P. aeruginosa and the Streptococcus milleri group (SMG). The results showed that CF-derived P. aeruginosa strains can infect the respiratory tract of adult mice, inducing local inflammation and lung damage. The severity of these infections was exacerbated when P. aeruginosa was co-administered with SMG. Notably, nebulization with the cell-free supernatant of L. plantarum produced beneficial effects, reducing respiratory infection severity and inflammatory responses induced by P. aeruginosa, both alone or in combination with SMG. Reduced bacterial loads and lung damage were observed in supernatant-treated mice compared to controls. Although further mechanistic studies are necessary, the results show that the cell-free supernatant of L. plantarum ATCC® 10241TM is an interesting adjuvant alternative to treat P. aeruginosa respiratory infections and superinfections in CF patients.
期刊介绍:
Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.