In Vitro Culture of Human Dermal Fibroblasts on Novel Electrospun Polylactic Acid Fiber Scaffolds Loaded with Encapsulated Polyepicatechin Physical Gels.

IF 5 3区 化学 Q1 POLYMER SCIENCE Gels Pub Date : 2024-09-20 DOI:10.3390/gels10090601
Eliza Miranda-Buendia, Gertrudis H González-Gómez, Alfredo Maciel-Cerda, Maykel González-Torres
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Abstract

Polyepicatechin (PEC) in a hydrogel has previously shown promise in enhancing physiological properties and scaffold preparation. However, it remains unclear whether PEC-based fibers can be applied in skin tissue engineering (STE). This study aimed to synthesize and characterize electrospun PEC physical gels and polylactic acid (PLA) scaffolds (PLAloadedPECsub) for potential use as constructs with human dermal fibroblasts (HDFs). PEC was produced through enzymatic polymerization, as confirmed by Fourier transform infrared (FTIR) spectroscopy. Scanning electron microscopy (SEM) demonstrated the feasibility of producing PLAloadedPECsub by electrospinning. The metabolic activity and viability of HDFs cocultured with the scaffolds indicate that PLAloadedPECsub is promising for the use of STE.

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新型电纺聚乳酸纤维支架上人真皮成纤维细胞的体外培养,支架上装有包裹型聚表儿茶素物理凝胶。
水凝胶中的聚表儿茶素(PEC)在增强生理特性和支架制备方面已显示出前景。然而,基于 PEC 的纤维能否应用于皮肤组织工程(STE)仍不清楚。本研究旨在合成和表征电纺 PEC 物理凝胶和聚乳酸(PLA)支架(PLAloadedPECsub),以作为人真皮成纤维细胞(HDFs)的潜在构建材料。经傅立叶变换红外光谱(FTIR)确认,PEC 是通过酶法聚合产生的。扫描电子显微镜(SEM)证明了通过电纺丝生产聚乳酸负载 PECsub 的可行性。与支架共培养的 HDFs 的代谢活性和存活率表明,PLAloadedPECsub 很有希望用于 STE。
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来源期刊
Gels
Gels POLYMER SCIENCE-
CiteScore
4.70
自引率
19.60%
发文量
707
审稿时长
11 weeks
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