Microbial decontamination of fresh-cut celery using simultaneous ultrasound and plasma-activated water treatment.

Abstract

This study investigated an ultrasound (US) treatment strategy in plasma-activated water (PAW) (UP treatment) to inactivate indigenous aerobic bacteria, Escherichia coli O157:H7, and Listeria monocytogenes in fresh-cut celery. Both plasma discharge and US treatment times contributed to the inactivation of indigenous bacteria in celery. The predicted optimal UP treatment conditions included a discharge time of 61.5 min and treatment time of 338 s, resulting in the inactivation of indigenous bacteria, E. coli O157:H7, and L. monocytogenes by 2.7, 1.7, and 3.2 log CFU/g, respectively. With an increase in plasma discharge time or US treatment time, the oxidation-reduction potential and electrical conductivity values of PAW increased, while the pH decreased. UP treatment effectively inactivated bacteria non-thermally, without altering the color of celery. Furthermore, UP treatment led to an increase in cell lipid peroxidation, reactive oxygen species production, and the number of non-viable E. coli O157:H7 and L. monocytogenes cells with membrane damage. This study highlights the potential of UP treatment for bacterial decontamination of fresh-cut celery.