Development of 5-fluorouracil/etoposide co-loaded electrospun nanofibrous scaffold for localized anti-melanoma therapy.

IF 3.1 4区 医学 Q2 BIOPHYSICS Journal of Applied Biomaterials & Functional Materials Pub Date : 2024-01-01 DOI:10.1177/22808000241284439
Shirin Shojaei, Mahtab Doostan, Hamidreza Mohammadi Motlagh, Seyedeh Sara Esnaashari, Hassan Maleki
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Abstract

Nanofibrous scaffolds have emerged as promising candidates for localized drug delivery systems in the treatment of cutaneous cancers. In this study, we prepared an electrospun nanofibrous scaffold incorporating 5-fluorouracil (5-FU) and etoposide (ETP) for chemotherapy targeting melanoma cutaneous cancer. The scaffold was composed of polyvinyl alcohol (PVA) and chitosan (CS), prepared via the electrospinning process and loaded with the chemotherapeutic agents. We conducted relevant physicochemical characterizations, assessed cytotoxicity, and evaluated apoptosis against melanoma A375 cells. The prepared 5-FU/ETP co-loaded PVA/CS scaffold exhibited nanofibers (NFs) with an average diameter of 321 ± 61 nm, defect-free and homogenous morphology. FTIR spectroscopy confirmed successful incorporation of chemotherapeutics into the scaffold. Additionally, the scaffold demonstrated a hydrophilic surface, proper mechanical strength, high porosity, and efficient liquid absorption capacity. Notably, sustained and controlled drug release was observed from the nanofibrous scaffold. Furthermore, the scaffold significantly increased cytotoxicity (95%) and apoptosis (74%) in A375 melanoma cells. Consequently, the prepared 5-FU/ETP co-loaded PVA/CS nanofibrous scaffold holds promise as a valuable system for localized eradication of cutaneous melanoma tumors and mitigation of adverse drug reactions associated with chemotherapy.

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开发用于局部抗黑色素瘤治疗的 5-氟尿嘧啶/埃托泊苷共载电纺纳米纤维支架。
纳米纤维支架已成为治疗皮肤癌的局部给药系统的理想候选材料。在这项研究中,我们制备了一种电纺纳米纤维支架,其中含有 5-氟尿嘧啶(5-FU)和依托泊苷(ETP),用于针对黑色素瘤皮肤癌的化疗。该支架由聚乙烯醇(PVA)和壳聚糖(CS)组成,通过电纺工艺制备,并添加了化疗药物。我们进行了相关的物理化学表征,评估了细胞毒性,并评价了黑色素瘤 A375 细胞的凋亡情况。制备的 5-FU/ETP 共负载 PVA/CS 支架呈现出平均直径为 321 ± 61 nm 的纳米纤维(NF),形态无缺陷且均匀。傅立叶变换红外光谱证实了化疗药物成功加入支架。此外,该支架还具有亲水性表面、适当的机械强度、高孔隙率和高效的液体吸收能力。值得注意的是,从纳米纤维支架中观察到了持续、可控的药物释放。此外,该支架还显著提高了 A375 黑色素瘤细胞的细胞毒性(95%)和凋亡率(74%)。因此,制备的 5-FU/ETP 共负载 PVA/CS 纳米纤维支架有望成为局部根治皮肤黑色素瘤肿瘤和减轻化疗相关药物不良反应的重要系统。
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来源期刊
Journal of Applied Biomaterials & Functional Materials
Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL
CiteScore
4.40
自引率
4.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics
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