In Vitro Wound-Healing Potential of Phenolic and Polysaccharide Extracts of Aloe vera Gel.

IF 5 3区 医学 Q1 ENGINEERING, BIOMEDICAL Journal of Functional Biomaterials Pub Date : 2024-09-13 DOI:10.3390/jfb15090266
Andreea Iosageanu, Elena Mihai, Ana-Maria Seciu-Grama, Elena Utoiu, Alexandra Gaspar-Pintiliescu, Florentina Gatea, Anisoara Cimpean, Oana Craciunescu
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Abstract

The present study aimed to conduct a comparative investigation of the biological properties of phenolic and polysaccharide extracts obtained using an ultrasound-assisted technique from Aloe vera gel and their effects on each stage of the wound healing process in in vitro experimental models. HPLC analysis showed that the phenolic extract contained aloin, ferulic, and caffeic acid, as well as quercetin dihydrate, as major compounds. Capillary zone electrophoresis indicated the prevalence of mannose and glucose in the polysaccharide extract. Cell culture testing revealed the anti-inflammatory properties of the phenolic extract at a concentration of 0.25 mg/mL through significant inhibition of pro-inflammatory cytokines-up to 28% TNF-α and 11% IL-8 secretion-in inflamed THP-1-derived macrophages, while a pro-inflammatory effect was observed at 0.5 mg/mL. The phenolic extract induced 18% stimulation of L929 fibroblast proliferation at a concentration of 0.5 mg/mL, enhanced the cell migration rate by 20%, and increased collagen type I synthesis by 18%. Moreover, the phenolic extract exhibited superior antioxidant properties by scavenging free DPPH (IC50 of 2.50 mg/mL) and ABTS (16.47 mM TE/g) radicals, and 46% inhibition of intracellular reactive oxygen species (ROS) production was achieved. The polysaccharide extract demonstrated a greater increase in collagen synthesis up to 25%, as well as antibacterial activity against Staphylococcus aureus with a bacteriostatic effect at 25 mg/mL and a bactericidal one at 50 mg/mL. All these findings indicate that the phenolic extract might be more beneficial in formulations intended for the initial phases of wound healing, such as inflammation and proliferation, while the polysaccharide extract could be more suitable for use during the remodeling stage. Moreover, they might be combined with other biomaterials, acting as efficient dressings with anti-inflammatory, antioxidant, and antibacterial properties for rapid recovery of chronic wounds.

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芦荟凝胶酚类和多糖提取物的体外伤口愈合潜力。
本研究旨在对利用超声辅助技术从芦荟凝胶中提取的酚类和多糖类提取物的生物特性及其对体外实验模型中伤口愈合过程各阶段的影响进行比较研究。高效液相色谱分析显示,酚类提取物中含有芦荟素、阿魏酸、咖啡酸以及二水槲皮素等主要化合物。毛细管区带电泳显示,多糖提取物中普遍含有甘露糖和葡萄糖。细胞培养测试表明,浓度为 0.25 mg/mL 的酚类提取物具有抗炎特性,能显著抑制促炎细胞因子--28% 的 TNF-α 和 11% 的 IL-8 在发炎的 THP-1 衍生巨噬细胞中的分泌,而浓度为 0.5 mg/mL 时则有促炎作用。在 0.5 mg/mL 浓度下,酚提取物对 L929 成纤维细胞增殖的刺激率为 18%,细胞迁移率提高了 20%,I 型胶原蛋白的合成增加了 18%。此外,酚类提取物通过清除游离 DPPH(IC50 为 2.50 mg/mL)和 ABTS(16.47 mM TE/g)自由基表现出卓越的抗氧化性,对细胞内活性氧(ROS)产生的抑制率达到 46%。多糖提取物显示胶原蛋白合成增加了 25%,并对金黄色葡萄球菌具有抗菌活性,25 毫克/毫升时具有抑菌作用,50 毫克/毫升时具有杀菌作用。所有这些发现都表明,酚类提取物可能更适合用于伤口愈合初期的配方,如炎症和增殖阶段,而多糖提取物可能更适合用于重塑阶段。此外,它们还可以与其他生物材料相结合,作为具有消炎、抗氧化和抗菌特性的高效敷料,用于慢性伤口的快速恢复。
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来源期刊
Journal of Functional Biomaterials
Journal of Functional Biomaterials Engineering-Biomedical Engineering
CiteScore
4.60
自引率
4.20%
发文量
226
审稿时长
11 weeks
期刊介绍: Journal of Functional Biomaterials (JFB, ISSN 2079-4983) is an international and interdisciplinary scientific journal that publishes regular research papers (articles), reviews and short communications about applications of materials for biomedical use. JFB covers subjects from chemistry, pharmacy, biology, physics over to engineering. The journal focuses on the preparation, performance and use of functional biomaterials in biomedical devices and their behaviour in physiological environments. Our aim is to encourage scientists to publish their results in as much detail as possible. Therefore, there is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Several topical special issues will be published. Scope: adhesion, adsorption, biocompatibility, biohybrid materials, bio-inert materials, biomaterials, biomedical devices, biomimetic materials, bone repair, cardiovascular devices, ceramics, composite materials, dental implants, dental materials, drug delivery systems, functional biopolymers, glasses, hyper branched polymers, molecularly imprinted polymers (MIPs), nanomedicine, nanoparticles, nanotechnology, natural materials, self-assembly smart materials, stimuli responsive materials, surface modification, tissue devices, tissue engineering, tissue-derived materials, urological devices.
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