Rapid Quantification of Oxytetracycline Based on Fluorescence Enhancement Influenced by pH.

IF 2.6 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Fluorescence Pub Date : 2024-09-26 DOI:10.1007/s10895-024-03941-0
Xiaodian Huang, Dong Yang, Liang Song, Yongcan Jiang
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Abstract

Accurate quantification of antibiotics in environmental samples is typically challenging due to the low antibiotic concentrations and the complexity of environmental matrices. This paper presents a fluorescence spectrometry method for determining oxytetracycline under alkaline conditions. The ionic distribution of the oxytetracycline solution was analyzed based on its dissociation constant. The dimethylamino group plays a crucial role in this method, as it promotes intramolecular charge transfer in the electronic excited state through its electron-donating capability with a lone electron pair. The presented method is straightforward, cost-effective, and holds potential for analyzing oxytetracycline in water sample after further investigation.

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基于 pH 值影响的荧光增强快速定量土霉素
由于抗生素浓度低、环境基质复杂,环境样本中抗生素的精确定量通常具有挑战性。本文介绍了一种在碱性条件下测定土霉素的荧光光谱法。根据土霉素的解离常数分析了土霉素溶液的离子分布。二甲基氨基在该方法中起着至关重要的作用,因为它通过孤电子对的电子供能能力促进了电子激发态下的分子内电荷转移。该方法简便易行、成本低廉,经进一步研究后有望用于分析水样中的土霉素。
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来源期刊
Journal of Fluorescence
Journal of Fluorescence 化学-分析化学
CiteScore
4.60
自引率
7.40%
发文量
203
审稿时长
5.4 months
期刊介绍: Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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