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Spectroscopic and TD-DFT Studies on Colourimetric Detection of Cyanide by a Triphenylphosphine-based Chemodosimeter Via ICT Modulation. ICT调制三苯基膦化学剂量计比色检测氰化物的光谱和TD-DFT研究。
IF 3.1 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-02-09 DOI: 10.1007/s10895-026-04724-5
S Sridharan, V Dharaniprabha, P Ponlakshmi, S Abirami, M Manjuladevi, K Satheshkumar, Kuppanagounder P Elango
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引用次数: 0
Convenient Preparation and Energy Transfer Kinetic Process Verification by Ultrafast Time-resolved Fluorescence Spectra for Natural Cryptophyte Phycocyanin 645. 天然隐植物藻蓝蛋白645的制备及能量传递动力学过程的超快时间分辨荧光光谱验证。
IF 3.1 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-02-09 DOI: 10.1007/s10895-025-04663-7
Yang Pu, Chuanxu Liu, Xiangyu Lan, Chao Xiao, Mingyuan Xie, Wenjun Li, Min Chen
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引用次数: 0
Chiral Ratiometric Fluorescent Silicon Nanoparticles for "Turn-on" Enantioselective Recognition of Tryptophan. 手性比例荧光硅纳米颗粒对色氨酸的“开启”对映选择性识别。
IF 3.1 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-02-07 DOI: 10.1007/s10895-025-04704-1
Chen Liu, Yaning Duan, Cancan Lu, Yu Fu, Cuiling Ren, Hongli Chen
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引用次数: 0
Evaluation of Artificial Intelligence-Generated DNA Aptamers Against Treponema pallidum Surface Proteins. 人工智能生成的抗梅毒螺旋体表面蛋白DNA适体的评价。
IF 3.1 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-02-06 DOI: 10.1007/s10895-026-04717-4
John G Bruno, Shamsudin Nasaev, Dmitry Ufaev, Jeffrey C Sivils

DNA aptamer sequences were selected in silico with assistance from artificial intelligence (AI) using the new Xelari.com platform against three known immunogenic surface proteins of Treponema pallidum designated Tpp17, Tpp47 and Tp0751. The in silico aptamers were synthesized with 5' Alexa Fluor 647 labels and shown to bind live Treponema pallidum by fluorescence microscopy and spectrofluorometry. While AI-predicted Kd values differed somewhat from measured Kd values, it is clear that the aptamers bound the T. pallidum surface which is consistent with the molecular docking models for each aptamer with each cognate target protein all of which are thought to be largely accessible for binding (i.e., not predominately embedded in the outer membrane or cell wall). All three aptamers also demonstrated good specificity in cross-reactivity binding studies.

在人工智能(AI)的帮助下,利用新的Xelari.com平台对三种已知的梅毒螺旋体表面免疫原性蛋白Tpp17、Tpp47和Tp0751进行了DNA适体序列的计算机筛选。用5' Alexa Fluor 647标记合成了硅适体,并通过荧光显微镜和荧光光谱法显示了与活体梅毒螺旋体的结合。虽然人工智能预测的Kd值与实际测量的Kd值有所不同,但很明显,适配体结合了T. pallidum表面,这与每个适配体与每个同源靶蛋白的分子对接模型是一致的,所有这些都被认为在很大程度上可以结合(即,不主要嵌入外膜或细胞壁)。这三种适体在交叉反应性结合研究中也表现出良好的特异性。
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引用次数: 0
Strategies for Cr(VI) Detection and Cell Imaging Using Yellow Fluorescence N-doped Carbon Dots. 黄色荧光n掺杂碳点的Cr(VI)检测和细胞成像策略
IF 3.1 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-02-06 DOI: 10.1007/s10895-025-04706-z
Huijing Hou, Qian Du, Yaqin Zhao, Xiping Mei, Junfen Li

Herein, multifunctional fluorescent N-doped carbon dots (N-CDs) are synthesized from O-phenylenediamine and 4-aminobenzoic acid. Hydrothermal synthesis and nitrogen doping make the N-CDs possess good physical and chemical properties with bright emission at 567 nm (QY = 32%) and present obvious excitation dependence. Among common metal ions, only Cr(VI) strongly and linearly decrease the yellow fluorescence of N-CDs in wide concentration ranges of 20.0-180 µM and 180-350 µM. Thus, N-CDs are constructed as efficient fluorescence probe for Cr(VI) with detection limit of 1.94 µM. The mechanism of quenching is indicated as the inner filter effect (IFE). The proposed sensor has been successfully applied in two kinds of water samples. In addition, the CDs are tried to apply for cell imaging. Above experiments prove that N-CDs based sensors are efficient strategies for sensitive Cr(VI) detection and cell imaging.

本文以邻苯二胺和4-氨基苯甲酸为原料合成了多功能荧光n掺杂碳点(N-CDs)。水热合成和氮掺杂使N-CDs具有良好的物理化学性能,在567 nm处发光明亮(QY = 32%),且具有明显的激发依赖性。在常见的金属离子中,只有Cr(VI)在20.0 ~ 180µM和180 ~ 350µM的较宽浓度范围内,对N-CDs的黄色荧光有较强的线性降低作用。因此,构建了N-CDs作为Cr(VI)的高效荧光探针,检测限为1.94µM。淬火机理为内滤效应(IFE)。该传感器已成功应用于两种水样中。此外,还尝试将cd应用于细胞成像。以上实验证明,基于N-CDs的传感器是Cr(VI)敏感检测和细胞成像的有效策略。
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引用次数: 0
A Dual-Organelle Fluorescent Probe for Visualizing Intracellular Microviscosity Dynamics. 用于观察细胞内微粘度动力学的双细胞器荧光探针。
IF 3.1 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-02-05 DOI: 10.1007/s10895-026-04714-7
Kaiwen Zheng, Xiang Wang, Haijiao Xie

Intracellular microviscosity is a key physicochemical parameter that influences molecular diffusion, signal transduction, and organelle function. However, fluorescence probes capable of selectively visualizing viscosity dynamics with high sensitivity and biological compatibility remain limited. Here, we report a dihydroxanthene-based viscosity-responsive fluorescent probe, ZKW, constructed with a D-π-A push-pull architecture and an o-bromophenyl molecular rotor. ZKW displays a pronounced viscosity-dependent fluorescence enhancement at 580 nm upon 550 nm excitation, originating from restricted intramolecular rotation and suppressed TICT processes. The probe exhibits good selectivity toward viscosity with negligible interference from polarity, pH, reactive species, metal ions, amino acids, or proteins, along with acceptable photostability and low cytotoxicity. Colocalization studies revealed preferential mitochondrial accumulation with partial lysosomal distribution, enabling simultaneous visualization of viscosity variations in these organelles. Using multiple cellular stress models-including LPS stimulation, oleic-acid treatment, nystatin exposure, and erastin-induced ferroptosis-ZKW effectively reported viscosity elevations at the subcellular level. These results establish ZKW as a robust and versatile tool for probing microviscosity heterogeneity in living cells and for facilitating studies of viscosity-associated physiological and pathological processes.

细胞内微粘度是影响分子扩散、信号转导和细胞器功能的关键理化参数。然而,能够选择性地观察高灵敏度和生物相容性的粘度动力学的荧光探针仍然有限。本文报道了一种基于二羟基蒽的粘度响应荧光探针ZKW,该探针具有D-π-A推挽结构和邻溴苯基分子转子。ZKW在550nm激发下,在580nm处显示出明显的黏度依赖性荧光增强,这是由于限制分子内旋转和抑制TICT过程造成的。该探针对粘度具有良好的选择性,极性、pH值、活性物质、金属离子、氨基酸或蛋白质的干扰可以忽略不计,同时具有可接受的光稳定性和低细胞毒性。共定位研究揭示了线粒体优先积累与部分溶酶体分布,使这些细胞器的粘度变化同时可视化。通过多种细胞应激模型——包括LPS刺激、油酸处理、制霉菌素暴露和erastin诱导的死铁——zkw有效地报告了亚细胞水平的黏度升高。这些结果表明,ZKW是一种强大而通用的工具,可用于探测活细胞中的微粘度异质性,并促进与粘度相关的生理和病理过程的研究。
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引用次数: 0
Perylene/4-Nitrophenol Based Green Light Emitting Luminophors as Efficient Hole-Transport Materials for Organic Optoelectronics. 基于苝/4-硝基苯酚的绿色发光材料作为有机光电子学中的高效空穴传输材料
IF 3.1 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-02-05 DOI: 10.1007/s10895-025-04705-0
K G Mane, A J Ghoti, P B Nagore
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引用次数: 0
Taming Light with Carbon Dots. 用碳点驯服光。
IF 3.1 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-02-04 DOI: 10.1007/s10895-026-04707-6
Arun M, Karthikeyan Alagarsamy, T C Sabri Girisun, Kannan Prabha, Murugan Perumal, Archana Pandi, Ramalingam Gopal

The breakthrough of high-power lasers urged the effective light quenching materials for safeguarding eyes,sensors and photo detectors. Encountered exploration of novel carbon dots to manipulate the ray of light at a modest price. Nonetheless, a carbon dot suffers from a large spectral range detection of a ray of light, so the research has been devoted to exploring the materials to cover different spectral ranges. This article reports a novel dual-photon emissive carbon dot with large spectral emission coverage with an effortless preparation strategy. The rapid fabrication procedure constituted with dualcolour emissive carbon dots(MCCD) in a single solvent with utilization of biomass source. We have identified the mono-solvent effect induced dual emissive carbon dots observed in fluorescence spectroscopy. The fluorescence carbon particle displays the nature of excitation-independent behaviour, with an average granule size of 16.93 nm, result obtained via Transmission electron microscopy. The carbon structure suffered from the lower carbonization temperature with rapid microwave treatment, causing a turbostratic carbon structure. The dual colour contains with NIR region bright red emission solution tested against Z-scan non-linear properties, it exposes the excited state absorption involved in two-photon absorption. It offers an approach for optical limiting behaviour, with threshold reaches up to 0.53 × 10- 10 and 4.54 × 1012 W/m2 was observed for MCCD. These result in a gateway for eco-friendly carbon dots into an optical limiting appeal.

高功率激光器的突破,迫切需要有效的光猝灭材料来保护眼睛、传感器和光电探测器。遇到了探索新的碳点,以适度的价格操纵光线。然而,碳点对一束光线的探测光谱范围较大,因此研究一直致力于探索覆盖不同光谱范围的材料。本文报道了一种新型的双光子发射碳点,该碳点具有大的光谱发射覆盖范围。利用生物质原料,在单一溶剂中制备双色发光碳点(MCCD)。我们在荧光光谱中发现了单溶剂效应诱导的双发射碳点。透射电镜结果显示,荧光碳颗粒具有非激发性,平均粒径为16.93 nm。快速微波处理使炭化温度降低,使碳结构形成涡层结构。双色含近红外区亮红色发射溶液,经z -扫描非线性特性测试,揭示了双光子吸收涉及的激发态吸收。它提供了一种光学限制行为的方法,阈值高达0.53 × 10- 10, MCCD观察到4.54 × 1012 W/m2。这些结果为环保碳点进入光学限制吸引力提供了一个门户。
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引用次数: 0
Phosphorus-Nitrogen Co-doped Carbon Dots for Fluorescence Detection of Tigecycline: Mechanistic Exploration. 磷氮共掺杂碳点荧光检测替加环素的机理探讨。
IF 3.1 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-02-04 DOI: 10.1007/s10895-025-04662-8
Qian Su, Li Huang, Youjin Zhang, Zhiyong Yang
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引用次数: 0
Structural Studies and Synthesis of a New 2-(1-Methyl-3-(o-tolyl)ureido)-N-(o-tolyl)acetamide: Binding Interaction With Bovine Gamma Globulin and DFT Investigation. 新型2-(1-甲基-3-(邻苯基)脲基)- n -(邻苯基)乙酰胺的结构研究与合成:与牛γ球蛋白的结合作用及DFT研究。
IF 3.1 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Pub Date : 2026-02-04 DOI: 10.1007/s10895-025-04694-0
Arul Murugesan, Thangaraj Thiruppathiraja, Karuppasamy Nandhini, Krishnamoorthy Shanmugaraj, Senthilkumar Lakshmipathi, Malaichamy Ilanchelian
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引用次数: 0
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Journal of Fluorescence
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