Direct effects of Xenorhabdus spp. cell-free supernatant on Meloidogyne incognita in tomato plants and its impact on entomopathogenic nematodes

Abstract

Entomopathogenic Xenorhabdus spp. bacteria, symbiont of the nematode Steinernema spp., shows potential for mitigating agricultural pests and diseases through bioactive compound production. The plant-parasitic nematode (PPN) Meloidogyne incognita affects the yield and quality of numerous crops, causing significant economic losses. We speculate that Cell-Free Supernatants (CFS) from Xenorhabdus spp. could reduce the impact of the root-knot nematode (RKN) M. incognita without negatively affecting entomopathogenic nematodes (EPNs), which are considered beneficial organisms. This study explored the activity of seven CFS against M. incognita (two populations, AL05 and Chipiona) and their possible effects on EPNs. The in vitro impact of CFS at 10 %, 40 %, and 90 % concentrations on nematode motility at four and 24 h were tested on the PPN M. incognita and two EPNs, S. feltiae and H. bacteriophora. Additionally, EPN viability and virulence were evaluated at two and five days. On the other hand, tomato plant-mesocosm experiments examined the activity of four CFS on M. incognita reproductive capacity and EPN virulence. In vitro exposure of M. incognita to 90 % concentration of CFS resulted in reductions of activity over 60 % after four hours of expossure in four out of seven CFS. In the in vitro evaluation of two species of EPNs, none of the CFS affected the activity across any tested doses after four hours of exposure nor after 24 h. Plant-mesocosm experiments showed that CFS application significantly reduced RKN galls, egg masses, and galling index. However, the virulence of both EPN species decreased 15 days after application, with a significant impact on S. feltiae. Overall, these findings suggest that CFS could be used as a bio-tool against M. incognita in tomato crops, mitigating its impact on plant growth. However, this study also highlights the necessity of investigating the effects of CFS on non-target organisms.