Occurrence, virulence, and AMR profile of Vibrio parahaemolyticus isolated from shellfish growing areas located along the south-west coast of India.

IF 2.5 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES Journal of water and health Pub Date : 2024-09-01 Epub Date: 2024-08-06 DOI:10.2166/wh.2024.338
Ally C Antony, Reshma Silvester, P A Aneesa, Vipindas P V, Deborah Gnana Selvam A, Vivas Salim, Mini K Paul, Mohamed Hatha Abdulla
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Abstract

Vibrio parahaemolyticus is a leading cause of human gastroenteritis associated with seafood consumption. The present study aimed to investigate the occurrence and risk assessment of V. parahaemolyticus isolated from live Indian black clams, sediment, and water samples collected from shellfish harvesting areas located along the south-west coast of India. Out of the total 72 samples collected, 55.6% revealed the presence of V. parahaemolyticus; the highest occurrence was observed in shellfish samples. The presence of tdh and trh virulence genes was screened by multiplex PCR. Virulence genes could be detected in 25.8% of the strains; 19.35% of them were trh positive and 3.2% were tdh positive, while 3.2% of strains exhibited the coexistence of both virulence genes. Antimicrobial resistance (AMR) determined by the disk diffusion method revealed that 87% of the strains were multiple drug resistant and exhibited 21 diverse resistance patterns. The overall multiple antibiotic resistance (MAR) index values ranged from 0 to 0.8. To the best of our knowledge, this is the first report to document the presence of pathogenic and multidrug-resistant V. parahaemolyticus in shellfish harvesting areas of the Indian sub-continent. The study reveals possible health hazards associated with consuming shellfish harvested from the study area.

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从印度西南海岸贝类生长区分离出的副溶血性弧菌的发生、毒性和 AMR 特征。
副溶血性弧菌是与食用海产品有关的人类肠胃炎的主要病因。本研究旨在调查从印度西南海岸贝类捕捞区采集的活体印度黑蛤、沉积物和水样中分离出的副溶血性弧菌的发生情况和风险评估。在收集到的 72 个样本中,55.6% 发现了副溶血性弧菌;贝类样本中副溶血性弧菌的出现率最高。通过多重 PCR 检测了tdh 和 trh 毒力基因的存在。25.8%的菌株可检测到毒力基因;其中19.35%为trh阳性,3.2%为tdh阳性,3.2%的菌株同时存在这两种毒力基因。用磁盘扩散法测定的抗菌药耐药性(AMR)显示,87%的菌株具有多重耐药性,并表现出 21 种不同的耐药性模式。总的多重抗生素耐药性(MAR)指数值从 0 到 0.8 不等。据我们所知,这是第一份记录印度次大陆贝类捕捞区存在致病性和耐多药副溶血性弧菌的报告。这项研究揭示了食用从研究地区捕捞的贝类可能对健康造成的危害。
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来源期刊
Journal of water and health
Journal of water and health 环境科学-环境科学
CiteScore
3.60
自引率
8.70%
发文量
110
审稿时长
18-36 weeks
期刊介绍: Journal of Water and Health is a peer-reviewed journal devoted to the dissemination of information on the health implications and control of waterborne microorganisms and chemical substances in the broadest sense for developing and developed countries worldwide. This is to include microbial toxins, chemical quality and the aesthetic qualities of water.
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