Jacketed telemetry in rats: a novel non-invasive method for cardiorespiratory phenotyping during treadmill exercise.

IF 1.3 4区 农林科学 Q2 VETERINARY SCIENCES Laboratory Animals Pub Date : 2024-09-30 DOI:10.1177/00236772241259857
Stéphane Tanguy, Agathe Cambier, Leandro Fontana-Pires, Timothé Flenet, Charles Eynard, Julie Fontecave-Jalon, Pierre-Yves Gumery, François Boucher
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Abstract

The development of alternative methods for monitoring cardiorespiratory function without restraint or surgical implantation is attracting growing interest for both ethical and scientific reasons. For this purpose, a new non-invasive jacketed telemetry tool consisting in a radio device maintained in a jacket worn by the animal was previously developed to improve cardiorespiratory monitoring. It allows simultaneous monitoring of cardiac activity by surface electrocardiagram, respiratory function by respiratory inductive plethysmography, and locomotor activity by accelerometry. However, this tool has only been validated under conditions of low/intermediate activity levels or in anesthetized animals. This study aimed to evaluate the feasibility of using this system in the challenging conditions of an exertion protocol. Male Wistar rats (n = 10, 8-9 weeks old) were subjected to an incremental treadmill exercise protocol including speed levels from 5 to 40 cm s-1 separated by 30-s breaks. Heart rate (HR) and minute ventilation (assessed by minute volume; MV) were continuously monitored. At the end of each running level and during the 30-s breaks, HR and MV showed a significant increase compared to resting values. They returned to the baseline within 60 min of post-exercise recovery. Overall, our results demonstrated (i) the ability of the animal to run while wearing the device and (ii) the ability of the device to reliably monitor cardiorespiratory adaptation to treadmill exercise despite significant mechanical disturbances. In conclusion, this study highlights the possibility of non-invasively monitoring cardiorespiratory functional variables that were previously unattainable under conditions of high activity in freely moving animals.

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大鼠夹套遥测:跑步机运动中心肺表型的新型无创方法。
出于伦理和科学原因,开发无需束缚或手术植入的心肺功能监测替代方法正引起越来越多的关注。为此,我们开发了一种新型非侵入式外套遥测工具,该工具由一个无线电装置组成,安装在动物所穿的外套中,以改善心肺功能监测。它可以通过表面心电图同时监测心脏活动,通过呼吸感应式胸压计同时监测呼吸功能,通过加速度计同时监测运动活动。然而,该工具仅在低/中等活动水平条件下或在麻醉动物中进行过验证。本研究旨在评估在具有挑战性的用力方案条件下使用该系统的可行性。雄性 Wistar 大鼠(n = 10,8-9 周大)接受了递增式跑步机运动方案,包括速度水平从 5 厘米/秒到 40 厘米/秒,中间休息 30 秒。连续监测心率(HR)和分钟通气量(以分钟容量评估;MV)。在每个跑步级别结束时和休息 30 秒期间,心率和每分钟通气量与静息值相比都有显著增加。在运动后恢复的 60 分钟内,它们又恢复到基线值。总之,我们的研究结果表明:(i) 动物有能力在佩戴设备的情况下跑步;(ii) 尽管存在明显的机械干扰,设备仍能可靠地监测心肺功能对跑步机运动的适应性。总之,这项研究强调了无创监测心肺功能变量的可能性,而这在以前自由活动的动物的高活动量条件下是无法实现的。
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来源期刊
Laboratory Animals
Laboratory Animals 生物-动物学
CiteScore
4.90
自引率
8.30%
发文量
64
审稿时长
6-12 weeks
期刊介绍: The international journal of laboratory animal science and welfare, Laboratory Animals publishes peer-reviewed original papers and reviews on all aspects of the use of animals in biomedical research. The journal promotes improvements in the welfare or well-being of the animals used, it particularly focuses on research that reduces the number of animals used or which replaces animal models with in vitro alternatives.
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