Molecular detection and characterization of Trichomonas gallinae isolated from ornamental birds in Tehran, Iran.

IF 1.8 3区 医学 Q2 PARASITOLOGY Parasitology Research Pub Date : 2024-09-30 DOI:10.1007/s00436-024-08346-3
Aida Ebrahimi Ahmadabad, Bahar Shemshadi, Zohreh Momeni, Nadia Taeifi Nasrabadi
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Abstract

Trichomonas gallinae is a widespread protozoan parasite that primarily affects birds, causing a disease known as avian trichomonosis. The present study aimed to investigate the prevalence and genetic diversity of T. gallinae, a parasite causing avian trichomoniasis in feral pigeons, budgerigars, and finches in Tehran, Iran. The 5.8S ribosomal RNA locus, along with the internal transcribed spacer 2 (ITS2) region, has been extensively utilized for genotype identification and for determining inter- and intra-specific diversity. More recently, the Fe-hydrogenase (Fe-Hyd) gene has been suggested as an additional genetic marker to enhance the accuracy of strain subtyping discrimination. In the present study, a total of 12% (12/100) birds examined were infected with T. gallinae using microscopy and PCR methods. Infection was found in seven of 30 (23.3%) feral pigeons, three of 40 (7.5%) budgerigars, and two of 30 (6.66%) finches. Analysis of the ITS2 region of T. gallinae isolates revealed two highly similar sequences. The first sequence (GenBank: OQ689964-OQ689970) was found in five feral pigeons and two budgerigars, whereas the second sequence (GenBank: OQ689971-OQ689975) was identified in two feral pigeons, one budgerigar, and two finches. Phylogenetic analysis confirmed the presence of two distinct clusters (cluster I and cluster II) within the trichomonads based on the ITS2 region. However, further analysis using Fe-Hyd revealed greater diversity, with three subtypes identified (A1, A2, and C1). One isolate identified in the present study (GenBank accession number: OQ694508.1) belonged to subtype A1. Combining ITS2 and Fe-Hyd markers holds promise for a more comprehensive understanding of the population structure of T. gallinae and the potential role of ITS2 in host adaptation.

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从伊朗德黑兰观赏鸟中分离出的五倍子毛滴虫的分子检测和特征描述。
五倍子毛滴虫(Trichomonas gallinae)是一种广泛存在的原生动物寄生虫,主要影响鸟类,导致一种被称为禽毛滴虫病的疾病。本研究旨在调查伊朗德黑兰野鸽、虎皮鹦鹉和雀鸟中引起禽毛滴虫病的寄生虫 T. gallinae 的流行情况和遗传多样性。5.8S 核糖体 RNA 位点以及内部转录间隔 2(ITS2)区域已被广泛用于基因型鉴定以及确定种间和种内多样性。最近,有人建议将铁氢化酶(Fe-Hyd)基因作为额外的遗传标记,以提高菌株亚型鉴别的准确性。在本研究中,使用显微镜和聚合酶链反应(PCR)方法,共有 12% 的鸟类(12/100)感染了五倍子蝇。30只野鸽中有7只(23.3%)、40只虎皮鹦鹉中有3只(7.5%)、30只雀鸟中有2只(6.66%)被感染。对 T. gallinae 分离物的 ITS2 区域分析发现了两个高度相似的序列。第一个序列(GenBank:OQ689964-OQ689970)在五只野鸽和两只虎皮鹦鹉中发现,而第二个序列(GenBank:OQ689971-OQ689975)在两只野鸽、一只虎皮鹦鹉和两只雀中发现。系统发育分析证实,基于 ITS2 区域,毛滴虫存在两个不同的群集(群集 I 和群集 II)。然而,使用铁-水化物(Fe-Hyd)进行的进一步分析显示了更大的多样性,确定了三个亚型(A1、A2 和 C1)。本研究中发现的一个分离株(GenBank登录号:OQ694508.1)属于 A1 亚型。结合 ITS2 和 Fe-Hyd 标记有望更全面地了解 T. gallinae 的种群结构以及 ITS2 在宿主适应中的潜在作用。
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来源期刊
Parasitology Research
Parasitology Research 医学-寄生虫学
CiteScore
4.10
自引率
5.00%
发文量
346
审稿时长
6 months
期刊介绍: The journal Parasitology Research covers the latest developments in parasitology across a variety of disciplines, including biology, medicine and veterinary medicine. Among many topics discussed are chemotherapy and control of parasitic disease, and the relationship of host and parasite. Other coverage includes: Protozoology, Helminthology, Entomology; Morphology (incl. Pathomorphology, Ultrastructure); Biochemistry, Physiology including Pathophysiology; Parasite-Host-Relationships including Immunology and Host Specificity; life history, ecology and epidemiology; and Diagnosis, Chemotherapy and Control of Parasitic Diseases.
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