Network analysis of metabolomics, transcriptome and hormones reveals propionic acid-mediated novel survival strategy against drought in wheat.

IF 5.4 2区 生物学 Q1 PLANT SCIENCES Physiologia plantarum Pub Date : 2024-09-01 DOI:10.1111/ppl.14551
Zongzhen Li, Yanhao Lian, Hui Guo, Chenxi Li, Yongzhe Ren, Zeyu Xin, Tongbao Lin, Zhiqiang Wang
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Abstract

Propionic acid (PA), a low-molecular-weight organic acid, is crucial to plant life metabolism. However, the regulatory mechanism of PA-mediated drought resistance in wheat remains largely unknown. Herein, we reported on a regulatory network of PA-mediated drought resistance in wheat using integrated transcriptome and metabolomics analysis and verified genes associated with drought resistance. Compared to the water-treated group, the application of PA alleviated the damage of drought by increasing plant water content, antioxidant enzyme activities and decreasing the malondialdehyde level (MDA). Transcriptome and metabolomics analysis revealed that PA triggered upregulation of key genes and metabolites, including TaBCAT, TaALDH6A1, TaALDH7A1, TaCHI, TaFLS, chrysin, and galangin, which were involved in valine, leucine and isoleucine degradation or flavonoid biosynthesis, respectively. In addition, the expression of genes encoding auxin-related transcription factors (TFs) strikingly increased, such as auxin/indoleacetic acid (AUX/IAA) and auxin response factor (ARF). Moreover, PA activated abscisic acid (ABA) and indole-3-acetic acid (IAA) signalling pathways. Taken together, our findings suggest that PA promotes energy metabolism and antioxidant activities to confer wheat drought resistance by introducing comprehensive and systemic effects of valine, leucine and isoleucine degradation flavonoid biosynthesis. Furthermore, activated AUX/IAA and ARF TFs might serve vital roles in drought resistance via modulating IAA signalling. This study provides novel insights into PA-mediated crop resistance and the improvement of the agroecological environment.

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代谢组学、转录组和激素的网络分析揭示了丙酸介导的小麦抗旱新生存策略。
丙酸(PA)是一种低分子量的有机酸,对植物的生命代谢至关重要。然而,PA 介导的小麦抗旱性的调控机制在很大程度上仍然未知。在此,我们利用转录组学和代谢组学的综合分析,报道了PA介导的小麦抗旱调控网络,并验证了与抗旱相关的基因。与水处理组相比,施用 PA 可提高植物含水量、抗氧化酶活性并降低丙二醛(MDA)水平,从而减轻干旱对小麦的伤害。转录组学和代谢组学分析表明,PA 引发了关键基因和代谢物的上调,包括 TaBCAT、TaALDH6A1、TaALDH7A1、TaCHI、TaFLS、菊苷和高良姜苷,它们分别参与了缬氨酸、亮氨酸和异亮氨酸的降解或黄酮类化合物的生物合成。此外,辅助素/吲哚乙酸(AUX/IAA)和辅助素响应因子(ARF)等与辅助素相关的转录因子(TFs)基因的表达量也显著增加。此外,PA 还激活了脱落酸(ABA)和吲哚-3-乙酸(IAA)信号通路。综上所述,我们的研究结果表明,PA 通过引入缬氨酸、亮氨酸和异亮氨酸降解类黄酮生物合成的综合系统效应,促进能量代谢和抗氧化活性,从而赋予小麦抗旱性。此外,激活的 AUX/IAA 和 ARF TFs 可能通过调节 IAA 信号在抗旱中发挥重要作用。这项研究为了解 PA 介导的作物抗性和改善农业生态环境提供了新的视角。
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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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