Assessment of porcine reproductive and respiratory syndrome virus (PRRSV) farm surface contamination through environmental sampling.

IF 3 2区 农林科学 Q1 VETERINARY SCIENCES Porcine Health Management Pub Date : 2024-09-27 DOI:10.1186/s40813-024-00387-5
Claudio Marcello Melini, Mariana Kikuti, Laura Bruner, Matt Allerson, Katie O'Brien, Chase Stahl, Brian Roggow, Paul Yeske, Brad Leuwerke, Mark Schwartz, Montserrat Torremorell, Cesar A Corzo
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Abstract

Background: During the fall of 2020, the porcine reproductive and respiratory syndrome virus (PRRSV) L1C.5 variant emerged and rapidly spread throughout southern Minnesota generating questions regarding possible transmission routes. This study aimed to investigate whether PRRSV could be detected on surfaces inside and outside pig barns housing L1C.5 variant PRRSV-positive pigs to illustrate the potential for indirect transmission of PRRSV. Seven Midwestern U.S. PPRS-positive breeding or growing pig farms and one PRRS-negative farm were conveniently selected. Internal and external barn surfaces were wiped using a PBS moistened cloth and the resulting liquid was submitted to the University of Minnesota Veterinary Diagnostic Laboratory for PRRSV RT-PCR testing and virus isolation.

Results: All (n = 26) samples from PRRSV-negative farm tested negative. Nineteen (13%) out of 143 samples from positive farms yielded positive RT-PCR results. Positive samples originated primarily from exhaust fan cones and doorknobs, followed by anteroom floor and mortality carts/sleds. Virus isolation attempted on two samples did not yield positive results.

Conclusions: PRRSV contamination can occur on surfaces inside and outside pig barns that are in frequent contact with farm personnel. Although virus isolation attempts were negative, our results illustrate the potential for PRRSV to be transmitted indirectly through contaminated materials or farm personnel. The study supports the implementation of biosecurity practices by farm personnel to prevent the introduction of PRRSV into farms and the prevention of PRRSV transmission between farms.

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通过环境采样评估猪繁殖与呼吸综合征病毒(PRRSV)对猪场表面的污染。
背景:2020 年秋季,猪繁殖与呼吸综合征病毒(PRRSV)L1C.5 变体出现并迅速蔓延至明尼苏达州南部,引发了有关可能传播途径的问题。本研究旨在调查在饲养 L1C.5 变异型 PRRSV 阳性猪的猪舍内外表面是否能检测到 PRRSV,以说明 PRRSV 间接传播的可能性。方便地选择了美国中西部七个 PPRS 阳性种猪场或生长猪场和一个 PRRS 阴性猪场。使用 PBS 润湿布擦拭猪舍内外表面,将擦拭后的液体送至明尼苏达大学兽医诊断实验室进行 PRRSV RT-PCR 测试和病毒分离:来自 PRRSV 阴性猪场的所有样本(n = 26)检测结果均为阴性。阳性猪场的 143 份样本中有 19 份(13%)RT-PCR 结果呈阳性。阳性样品主要来自排风扇锥体和门把手,其次是前厅地板和死亡率高的推车/雪橇。对两个样本进行病毒分离的尝试没有得到阳性结果:结论:PRRSV 污染可能发生在猪舍内外与猪场人员频繁接触的表面。尽管病毒分离尝试呈阴性,但我们的结果表明 PRRSV 有可能通过受污染的材料或猪场人员间接传播。该研究支持猪场人员实施生物安全措施,以防止 PRRSV 传入猪场,并防止 PRRSV 在猪场之间传播。
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来源期刊
Porcine Health Management
Porcine Health Management Veterinary-Food Animals
CiteScore
5.40
自引率
5.90%
发文量
49
审稿时长
14 weeks
期刊介绍: Porcine Health Management (PHM) is an open access peer-reviewed journal that aims to publish relevant, novel and revised information regarding all aspects of swine health medicine and production.
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