{"title":"Comparative analysis of protein profiles in skin secretions of some Rana species: Preliminary insights into antimicrobial activity","authors":"Ebru Tanrıverdi O, Dinçer Ayaz, Yiğit Terzi","doi":"10.1016/j.toxicon.2024.108110","DOIUrl":null,"url":null,"abstract":"<div><div>Protein profiles of skin secretions of <em>Rana dalmatina</em> (Agile Frog), <em>Rana macrocnemis</em> (Uludağ Frog), <em>Rana tavasensis</em> (Tavas Frog) and <em>Rana holtzi</em> (Taurus Frog) frog species belonging to the <em>Rana</em> genus distributed in the Anatolian region of Türkiye were determined for the first time using the Tricine-SDS-PAGE Electrophoresis method and Coomassie Brilliant Blue (CBB) staining. By the results, some peptides with mass ≤5 kDa were detected. Just one peptide with mass ≤5 kDa was found in the secretion of each <em>R. dalmatina</em>, <em>R. macrocnemis</em>, and <em>R. tavasensis</em> while there was two in <em>R. holtzi</em> secretion. The antibacterial activity of secretions was determined using plate well diffusion assay on <em>E. coli</em>, <em>S. typhimurium</em>, <em>S. aureus</em>, <em>B. cereus</em> and <em>L. monocytogenes</em> bacteria. <em>R. dalmatina</em> created the inhibition zone for <em>S. typhimurium, S. aureus, B. cereus,</em> and <em>L. monocytogenes.</em> The zones of inhibition by <em>R. tavasensis</em> and <em>R. macrocnemis</em> species secretions were observed on <em>S. aureus</em>, <em>B. cereus</em>, and <em>L. monocytogenes</em>. It was found that <em>R. holtzi</em> creates an inhibition zone only on <em>B. cereus.</em> The results showed that the secretion of none of the species doesn't have antibacterial activity on <em>E. coli</em>. The skin secretion of <em>R. dalmatina</em> showed the most activity against bacteria, while <em>R. holtzi</em> had the least.</div></div>","PeriodicalId":23289,"journal":{"name":"Toxicon","volume":"250 ","pages":"Article 108110"},"PeriodicalIF":2.6000,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxicon","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0041010124006822","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
引用次数: 0
Abstract
Protein profiles of skin secretions of Rana dalmatina (Agile Frog), Rana macrocnemis (Uludağ Frog), Rana tavasensis (Tavas Frog) and Rana holtzi (Taurus Frog) frog species belonging to the Rana genus distributed in the Anatolian region of Türkiye were determined for the first time using the Tricine-SDS-PAGE Electrophoresis method and Coomassie Brilliant Blue (CBB) staining. By the results, some peptides with mass ≤5 kDa were detected. Just one peptide with mass ≤5 kDa was found in the secretion of each R. dalmatina, R. macrocnemis, and R. tavasensis while there was two in R. holtzi secretion. The antibacterial activity of secretions was determined using plate well diffusion assay on E. coli, S. typhimurium, S. aureus, B. cereus and L. monocytogenes bacteria. R. dalmatina created the inhibition zone for S. typhimurium, S. aureus, B. cereus, and L. monocytogenes. The zones of inhibition by R. tavasensis and R. macrocnemis species secretions were observed on S. aureus, B. cereus, and L. monocytogenes. It was found that R. holtzi creates an inhibition zone only on B. cereus. The results showed that the secretion of none of the species doesn't have antibacterial activity on E. coli. The skin secretion of R. dalmatina showed the most activity against bacteria, while R. holtzi had the least.
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Toxicon''s "aims and scope" are to publish:
-articles containing the results of original research on problems related to toxins derived from animals, plants and microorganisms
-papers on novel findings related to the chemical, pharmacological, toxicological, and immunological properties of natural toxins
-molecular biological studies of toxins and other genes from poisonous and venomous organisms that advance understanding of the role or function of toxins
-clinical observations on poisoning and envenoming where a new therapeutic principle has been proposed or a decidedly superior clinical result has been obtained.
-material on the use of toxins as tools in studying biological processes and material on subjects related to venom and antivenom problems.
-articles on the translational application of toxins, for example as drugs and insecticides
-epidemiological studies on envenoming or poisoning, so long as they highlight a previously unrecognised medical problem or provide insight into the prevention or medical treatment of envenoming or poisoning. Retrospective surveys of hospital records, especially those lacking species identification, will not be considered for publication. Properly designed prospective community-based surveys are strongly encouraged.
-articles describing well-known activities of venoms, such as antibacterial, anticancer, and analgesic activities of arachnid venoms, without any attempt to define the mechanism of action or purify the active component, will not be considered for publication in Toxicon.
-review articles on problems related to toxinology.
To encourage the exchange of ideas, sections of the journal may be devoted to Short Communications, Letters to the Editor and activities of the affiliated societies.
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