Biological characteristics of a new long-chain fatty acid transport protein 1 from Trichinella spiralis and its participation in lipid metabolism, larval moulting, and development.

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES Veterinary Research Pub Date : 2024-09-30 DOI:10.1186/s13567-024-01380-0
Yang Li Li, Qi Qi Lu, Wen Wen Zheng, Zhao Yu Zhang, Jin Yi Wu, Mei Hao Wei, Xin Zhuo Zhang, Ruo Dan Liu, Zhong Quan Wang, Jing Cui
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Abstract

Long-chain fatty acid transport protein 1 (FATP1) is a member of the fatty acid transporter family. It facilitates transmembrane transport of fatty acids and participates in lipid metabolism. Lipids are essential components of the cell and organelle membranes of Trichinella spiralis. The nematode has lost the capacity to synthesise the necessary lipids de novo and has instead evolved to obtain fatty acids and their derivatives from its host. This study aims to ascertain the primary biological characteristics and roles of T. spiralis FATP1 (TsFATP1) in lipid metabolism, larval moulting, and the development of this nematode. The results show that TsFATP1 is highly expressed at enteral T. spiralis stages, mainly localised at the cuticle, the stichosome and the intrauterine embryos of the parasite. The silencing of the TsFATP1 gene by TsFATP1-specific dsRNA significantly decreases the expression levels of TsFATP1 in the worm. It reduces the contents of ATP, triglycerides, total cholesterol, and phospholipids both in vitro and in vivo. RNAi inhibits lipid metabolism, moulting, and the growth of this nematode. The results demonstrate that TsFATP1 plays an essential role in lipid metabolism, moulting, and the development of T. spiralis. It could also be a target candidate for the anti-Trichinella vaccine and drugs.

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一种新的螺旋毛霉长链脂肪酸转运蛋白 1 的生物学特征及其在脂质代谢、幼虫蜕皮和发育过程中的参与。
长链脂肪酸转运蛋白 1(FATP1)是脂肪酸转运体家族的成员。它能促进脂肪酸的跨膜转运,并参与脂质代谢。脂质是螺旋毛线虫细胞膜和细胞器膜的重要组成部分。这种线虫已经丧失了从头合成必要脂质的能力,而是进化到从宿主那里获取脂肪酸及其衍生物。本研究旨在确定旋毛线虫 FATP1(TsFATP1)在脂质代谢、幼虫蜕皮和线虫发育过程中的主要生物学特征和作用。研究结果表明,TsFATP1 在螺旋蓟马的肠道阶段高度表达,主要定位于寄生虫的角质层、支架体和宫内胚胎。用 TsFATP1 特异性 dsRNA 沉默 TsFATP1 基因会显著降低 TsFATP1 在蠕虫体内的表达水平。它能降低体外和体内 ATP、甘油三酯、总胆固醇和磷脂的含量。RNAi 可抑制该线虫的脂质代谢、蜕皮和生长。研究结果表明,TsFATP1 在螺旋体的脂质代谢、蜕皮和发育过程中发挥着重要作用。它也可能成为抗螺旋体疫苗和药物的候选靶标。
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来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
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