Analytical Validation of Two Point-of-Care Assays for Hematological Analysis in the Miranda Donkey.

IF 2 2区农林科学 Q2 VETERINARY SCIENCES Veterinary Sciences Pub Date : 2024-09-22 DOI:10.3390/vetsci11090450

Céline Costa, Ana Patrícia Sousa, Grasiene Silva, Felisbina Queiroga, Ângela Martins, Daniela Andrade, Ana C Silvestre-Ferreira

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Abstract

Miranda donkeys are an endangered autochthonous breed of Portugal. The HemoCue WBC DIFF and HemoCue Hb 201 portable analyzers, developed as a simplified alternative method for total and differential WBC count and hemoglobin measurement, respectively, may be relevant tools in veterinary practice. This study aimed to validate these instruments using Miranda donkey blood samples. For the HemoCue WBC DIFF, most parameters showed acceptable intra- and inter-assay precision with coefficients of variation (CV) below 5%, except for monocytes and eosinophils with higher CVs. The HemoCue Hb 201 showed CVs of 1.98% and 4.07%. Linearity correlation coefficients (r) ranged from 0.53 to 0.99 for HemoCue WBC DIFF and 0.99 for HemoCue Hb 201. Significant levels for neutrophils, lymphocytes, monocytes, eosinophils, and Hb measurements varied. Comparisons with ProCyte Dx showed an excellent correlation for WBC (r_s = 0.96), neutrophils (r_s = 0.91), lymphocytes (r_s = 0.94), and eosinophils (r_s = 0.90) but a poor correlation for monocytes and basophils. The HemoCue Hb 201 showed a correlation of r_s = 0.96 with ProCyte Dx. In conclusion, both analyzers provided reliable results and are suitable for use in Miranda's donkey breed for WBC counts and Hb measurements.

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用于米兰达驴血液学分析的两种护理点测定的分析验证

米兰达驴是葡萄牙濒临灭绝的本土品种。HemoCue WBC DIFF 和 HemoCue Hb 201 便携式分析仪是分别作为白细胞总数和差值以及血红蛋白测量的简化替代方法而开发的，可能是兽医实践中的相关工具。本研究旨在使用米兰达驴血样验证这些仪器。除了单核细胞和嗜酸性粒细胞的变异系数（CV）较高外，HemoCue WBC DIFF 的大多数参数显示出可接受的测定内和测定间精度，变异系数（CV）低于 5%。HemoCue Hb 201 的变异系数分别为 1.98% 和 4.07%。HemoCue WBC DIFF 的线性相关系数 (r) 为 0.53 至 0.99，HemoCue Hb 201 为 0.99。中性粒细胞、淋巴细胞、单核细胞、嗜酸性粒细胞和 Hb 测量的显著水平各不相同。与 ProCyte Dx 的比较显示，白细胞（rs = 0.96）、中性粒细胞（rs = 0.91）、淋巴细胞（rs = 0.94）和嗜酸性粒细胞（rs = 0.90）的相关性极佳，但单核细胞和嗜碱性粒细胞的相关性较差。HemoCue Hb 201 与 ProCyte Dx 的相关性为 rs = 0.96。总之，这两种分析仪都能提供可靠的结果，适用于米兰达驴种的白细胞计数和血红蛋白测量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Sciences VETERINARY SCIENCES-

CiteScore

2.90

自引率

8.30%

发文量

612

审稿时长

6 weeks

期刊介绍： Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.