Assessing the Relationship between proAKAP4 Level and Longevity of Sexed Sperm Quality after Thawing.

IF 2 2区农林科学 Q2 VETERINARY SCIENCES Veterinary Sciences Pub Date : 2024-09-21 DOI:10.3390/vetsci11090444

İlktan Bastan, Fırat Korkmaz, Derya Şahin, Seher Şimşek, Ufuk Kaya

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Abstract

ProAKAP4 is a sperm structural protein that regulates motility through the PKA-dependent cAMP signaling pathway, which is synthesized as an X chromosome-linked member of the gene family. This study aims to determine the optimal level of proAKAP4 for evaluating sexed semen through investigating its relationship with the longevity of sperm quality in sexed Holstein bull sperm. A total of 30 sexed sperm samples (bearing X chromosomes) from 30 distinct Holstein bulls (n = 30) were analyzed. The frozen bull sperm samples were assessed for their proAKAP4 levels, mitochondrial membrane potential, plasma membrane and acrosome integrity (PMAI), and spermatozoa movement parameters at hours 0 and 3 after thawing. The proAKAP4 levels in the sexed sperm samples ranged from 16.35 to 72.10 ng/10 M spz, with an average of 37.18 ± 15.1 ng/10 M spz. A strong positive correlation was observed between proAKAP4 levels and total motility, progressive motility, PMAI, high mitochondrial membrane potential, VAP, and VCL values after 3 h of incubation, when compared to post-thaw analyses. The results also reveal that spermatozoa with proAKAP4 levels of ≥40 ng/10 M spz exhibit higher quality. In conclusion, the level of proAKAP4 in sexed sperm aligns with previous studies and shows potential as a biomarker for assessing the longevity of sexed sperm quality.

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评估proAKAP4水平与解冻后性别精子质量寿命之间的关系

proAKAP4是一种精子结构蛋白，可通过依赖于PKA的cAMP信号通路调节精子的运动能力，它是作为基因家族中与X染色体相连的成员合成的。本研究旨在通过研究proAKAP4与荷斯坦公牛精子质量寿命的关系，确定评估性别精液的最佳proAKAP4水平。共分析了来自 30 头不同荷斯坦公牛（n = 30）的 30 份性别精子样本（带有 X 染色体）。对冷冻的公牛精子样本在解冻后 0 小时和 3 小时内的 proAKAP4 水平、线粒体膜电位、质膜和顶体完整性（PMAI）以及精子运动参数进行了评估。性别精子样本中的proAKAP4水平从16.35到72.10纳克/10毫微克不等，平均为37.18 ± 15.1纳克/10毫微克。与解冻后分析相比，培养 3 小时后，proAKAP4 水平与总运动能力、渐进运动能力、PMAI、高线粒体膜电位、VAP 和 VCL 值之间存在很强的正相关性。结果还显示，proAKAP4水平≥40 ng/10 M spz的精子质量更高。总之，性别精子中原AKAP4的水平与之前的研究结果一致，并显示出作为评估性别精子质量寿命的生物标志物的潜力。

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来源期刊

Veterinary Sciences VETERINARY SCIENCES-

CiteScore

2.90

自引率

8.30%

发文量

612

审稿时长

6 weeks

期刊介绍： Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.