A Novel Ferrocene-Linked Thionine as a Dual Redox Mediator for the Electrochemical Detection of Dopamine and Hydrogen Peroxide.

IF 4.9 3区工程技术 Q1 CHEMISTRY, ANALYTICAL Biosensors-Basel Pub Date : 2024-09-19 DOI:10.3390/bios14090448

Manikandan Palinci Nagarajan, Manikandan Ramalingam, Ilakeya Subbiah Arivuthilagam, Vishwa Paramaguru, Md Mahbubur Rahman, Jongdeok Park, Francis Kwaku Asiam, Byungjik Lee, Kwang Pyo Kim, Jae-Joon Lee

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Abstract

We introduce a novel dual redox mediator synthesized by covalently linking ferrocene dicarboxylic acid (FcDA) and thionine (TH) onto a pre-treated glassy carbon electrode. This unique structure significantly enhances the electro-oxidation of dopamine (DA) and the reduction of hydrogen peroxide (H₂O₂), offering a sensitive detection method for both analytes. The electrode exhibits exceptional sensitivity, selectivity, and stability, demonstrating potential for practical applications in biosensing. It facilitates rapid electron transfer between the analyte and the electrode surface, detecting H₂O₂ concentrations ranging from 1.5 to 60 µM with a limit of detection (LoD) of 0.49 µM and DA concentrations from 0.3 to 230 µM with an LoD of 0.07 µM. The electrode's performance was validated through real-sample analyses, yielding satisfactory results.

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二茂铁连接的新型硫氨酸作为电化学检测多巴胺和过氧化氢的双重氧化还原介质

我们介绍了一种新型双重氧化还原介质，它是通过将二茂铁二羧酸（FcDA）和亚硫酸（TH）共价连接到预先处理过的玻璃碳电极上而合成的。这种独特的结构大大增强了多巴胺（DA）的电氧化作用和过氧化氢（H2O2）的还原作用，为这两种分析物提供了一种灵敏的检测方法。该电极具有极高的灵敏度、选择性和稳定性，显示了在生物传感领域的实际应用潜力。它能促进分析物与电极表面之间的快速电子转移，检测 H2O2 浓度范围为 1.5 至 60 µM，检测限（LoD）为 0.49 µM；检测 DA 浓度范围为 0.3 至 230 µM，检测限（LoD）为 0.07 µM。通过实际样品分析验证了该电极的性能，结果令人满意。

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来源期刊

Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

6.60

自引率

14.80%

发文量

983

审稿时长

11 weeks

期刊介绍： Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.