Impedimetric Biosensor of Silver Molybdate Anchored on rGO Sheet for Detection of Breast Cancer Mucin-1 Biomarker

Abstract

This work aims to detect the Mucin-1 (MUC1) breast cancer biomarker using a label-free impedimetric biosensor of silver molybdate anchored on reduced graphene oxide (Ag₂MoO₄-rGO). A hydrothermal method has been employed to synthesize the Ag₂MoO₄-rGO nanocomposite. Subsequently, they have been studied through analytical characterization techniques to study the electrocatalytic activity, electroactive surface area, surface functionalities, and morphologies to determine the suitability of the material for biosensor fabrication. Results reveal that the Ag₂MoO₄-rGO nanocomposite possesses excellent electroconductivity and surface properties that have significant characteristics for the biosensor's performance. A biosensor has been fabricated by the immobilization of Ag₂MoO₄-rGO nanocomposite and anti-MUC1 antibody on a glassy carbon electrode. The electroanalytical studies revealed that the biosensor has excellent sensitivity and detection performance having a wide linearity of 100 fg mL⁻¹–5 ng mL⁻¹ and a low limit of detection of 2.70 fg mL⁻¹. Moreover, the remarkable selectivity, reproducibility, stability, and detection in serum samples determine the high performance of the biosensor. Therefore, the presented biosensor could be a promising diagnostic tool that shows the remarkable potential to efficiently detect the breast cancer MUC1 biomarker in patient's serum samples.