Pseudoalteromonas simplex sp. nov. Isolated from the Skin of Bandtail Puffer Fish (Sphoeroides spengleri).

IF 2.6 3区生物学 Q3 MICROBIOLOGY Current Microbiology Pub Date : 2024-10-02 DOI:10.1007/s00284-024-03905-4

Hannah K Mattsson, Mayanne A M de Freitas, Gustavo P R de Azevedo, Vinicius Salazar, Verônica V Vieira, Diogo A Tschoeke, Cristiane C Thompson, Fabiano L Thompson

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Abstract

A novel bacterial isolate A520^T (A520^T = CBAS 737^T = CAIM 1944^T) was obtained from the skin of bandtail puffer fish Sphoeroides spengleri (Tetraodontidae Family), collected in Arraial do Cabo (Rio de Janeiro, Brazil). A520^T is Gram-stain-negative, flagellated and aerobic bacteria. Optimum growth occurs at 25-30 °C in the presence of 3% NaCl. The genome sequence of the novel isolate consisted of 4.5 Mb (4082 coding genes and G+C content of 41.1%). The closest phylogenetic neighbor was Pseudoalteromonas shioyasakiensis JCM 18891^T (97.9% 16S rRNA sequence similarity, 94.8% Average Amino Acid Identity, 93% Average Nucleotide Identity and 51.8% similarity in Genome-to-Genome-Distance). Several in silico phenotypic features are useful to differentiate A520^T from its closest phylogenetic neighbors, including trehalose, D-mannose, cellobiose, pyrrolidonyl-beta-naphthylamide, starch hydrolysis, D-xylose, lactose, tartrate utilization, sucrose, citrate, glycerol, mucate and acetate utilization, malonate, glucose oxidizer, gas from glucose, nitrite to gas, L-rhamnose, ornithine decarboxylase, lysine decarboxylase and yellow pigment. The genome of the novel species contains 3 gene clusters (~ 66.81 Kbp in total) coding for different types of bioactive compounds that could indicate ecological roles pertaining to the bandtail puffer fish host. Based on genome-based taxonomic approach, strain A520^T (A520^T = CBAS 737^T = CAIM 1944^T) is proposed as a new species, Pseudoalteromonas simplex sp. nov.

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从带尾河豚（Sphoeroides spengleri）皮肤中分离出的新的假单胞菌（Pseudoalteromonas simplex sp.

一种新型细菌分离物 A520T（A520T = CBAS 737T = CAIM 1944T）取自在 Arraial do Cabo（巴西里约热内卢）采集的带尾河豚（四齿鱼科）的皮肤。A520T 是革兰氏染色阴性、鞭毛和需氧细菌。最适生长温度为 25-30 °C，存在 3% 的氯化钠。该新型分离菌的基因组序列为 4.5 Mb（4082 个编码基因，G+C 含量为 41.1%）。最近的系统发育邻居是 Pseudoalteromonas shioyasakiensis JCM 18891T（16S rRNA 序列相似度为 97.9%，平均氨基酸相同度为 94.8%，平均核苷酸相同度为 93%，基因组间距离相似度为 51.8%）。有几种硅表型特征有助于将 A520T 与其系统发育上的近邻区分开来，这些特征包括：三卤糖、D-甘露糖、纤维生物糖、吡咯烷酮基-beta-萘甲酰胺、淀粉水解、D-木糖、乳糖、酒石酸利用、蔗糖、酒石酸盐利用、蔗糖、柠檬酸盐、甘油、粘液酸盐和醋酸盐利用、丙二酸盐、葡萄糖氧化剂、葡萄糖产生的气体、亚硝酸盐转化为气体、L-鼠李糖、鸟氨酸脱羧酶、赖氨酸脱羧酶和黄色素。该新物种的基因组包含 3 个基因簇（总计约 66.81 Kbp），编码不同类型的生物活性化合物，这些化合物可能显示了与带尾河豚宿主有关的生态作用。根据基于基因组的分类方法，菌株 A520T（A520T = CBAS 737T = CAIM 1944T）被认为是一个新物种，即 Pseudoalteromonas simplex sp.

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.