Biocompatibility and osteogenic assessment of experimental fluoride-doped calcium-phosphate cements on human dental pulp stem cells

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Dental Materials Pub Date : 2024-11-01 DOI:10.1016/j.dental.2024.09.019

Carmela Del Giudice , Gianrico Spagnuolo , Ciro Menale , Yu Fu Chou , Juan Manuel Núñez Martí , Carlo Rengo , Sandro Rengo , Salvatore Sauro

{"title":"Biocompatibility and osteogenic assessment of experimental fluoride-doped calcium-phosphate cements on human dental pulp stem cells","authors":"Carmela Del Giudice , Gianrico Spagnuolo , Ciro Menale , Yu Fu Chou , Juan Manuel Núñez Martí , Carlo Rengo , Sandro Rengo , Salvatore Sauro","doi":"10.1016/j.dental.2024.09.019","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>This study investigated the impact of some specific experimental calcium phosphate cements doped with different fluoride salts (FDCPCs) concentrations on the basal functions of human Dental Pulp Stem Cells (hDPSCs). Furthermore, this study also examined the migration, as well as the mineralisation through osteogenic differentiation.</div></div><div><h3>Methods</h3><div>Experimental FDCPCs were formulated using different concentrations of calcium/sodium fluoride salts [(5 wt%: VS5F), (10 wt%: VS10F), (20 wt%: VS20F)]. A fluoride-free calcium phosphate (VS0F) was used as a control. The hDPSCs were assessed to evaluate their self-renewal and migration activity in the presence of eluates of the different FDCPCs. A viability assay in osteogenic conditions was carried out, along with the differentiation potential through Alkaline Phosphatase Activity (ALP), and Alizarin Red Staining (ARS). Moreover, the gene expression of specific markers (RUNX2, ALP, COL1α1, OCN, OPN, DSPP, MEPE, and DMP-1) was also evaluated.</div></div><div><h3>Results</h3><div>All the tested FDCPD had no influence on cell migrations, but they caused a decrease in cell viability in osteogenic conditions when not diluted. Conversely, the eluants of VS20F showed a positive effect on stem cell differentiation. This result was corroborated through ALP activity, ARS assay. Moreover, upregulation of specific gene markers such as RUNX2, DMP-1, and DSPP was observed in hDPSCs, especially when treated with VS20F.</div></div><div><h3>Significance</h3><div>The experimental FDCPC tested in this study exhibits a dose-dependent capacity to promote mineralisation in osteogenic environment. The FDCPC-VS20F seems to be the most promising experimental material suitable for developing of pulp-capping materials with osteogenic and bioactive properties.</div></div>","PeriodicalId":298,"journal":{"name":"Dental Materials","volume":"40 11","pages":"Pages 2043-2050"},"PeriodicalIF":4.6000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dental Materials","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0109564124002884","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives

This study investigated the impact of some specific experimental calcium phosphate cements doped with different fluoride salts (FDCPCs) concentrations on the basal functions of human Dental Pulp Stem Cells (hDPSCs). Furthermore, this study also examined the migration, as well as the mineralisation through osteogenic differentiation.

Methods

Experimental FDCPCs were formulated using different concentrations of calcium/sodium fluoride salts [(5 wt%: VS5F), (10 wt%: VS10F), (20 wt%: VS20F)]. A fluoride-free calcium phosphate (VS0F) was used as a control. The hDPSCs were assessed to evaluate their self-renewal and migration activity in the presence of eluates of the different FDCPCs. A viability assay in osteogenic conditions was carried out, along with the differentiation potential through Alkaline Phosphatase Activity (ALP), and Alizarin Red Staining (ARS). Moreover, the gene expression of specific markers (RUNX2, ALP, COL1α1, OCN, OPN, DSPP, MEPE, and DMP-1) was also evaluated.

Results

All the tested FDCPD had no influence on cell migrations, but they caused a decrease in cell viability in osteogenic conditions when not diluted. Conversely, the eluants of VS20F showed a positive effect on stem cell differentiation. This result was corroborated through ALP activity, ARS assay. Moreover, upregulation of specific gene markers such as RUNX2, DMP-1, and DSPP was observed in hDPSCs, especially when treated with VS20F.

Significance

The experimental FDCPC tested in this study exhibits a dose-dependent capacity to promote mineralisation in osteogenic environment. The FDCPC-VS20F seems to be the most promising experimental material suitable for developing of pulp-capping materials with osteogenic and bioactive properties.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

实验性掺氟磷酸钙水门汀对人牙髓干细胞的生物相容性和成骨性评估。

研究目的本研究调查了掺入不同浓度氟盐（FDCPCs）的特定实验性磷酸钙水泥对人类牙髓干细胞（hDPSCs）基础功能的影响。此外，本研究还考察了迁移以及通过成骨分化实现矿化的情况：方法：使用不同浓度的氟化钙/氟化钠盐[（5 wt%：VS5F）、（10 wt%：VS10F）、（20 wt%：VS20F）]配制实验性 FDCPCs。无氟磷酸钙（VS0F）用作对照。对 hDPSCs 进行了评估，以评价其在不同 FDCPCs 洗脱液存在下的自我更新和迁移活性。此外，还进行了成骨条件下的活力检测，以及通过碱性磷酸酶活性（ALP）和茜素红染色（ARS）检测分化潜力。此外，还评估了特定标记物（RUNX2、ALP、COL1α1、OCN、OPN、DSPP、MEPE 和 DMP-1）的基因表达：结果：所有测试的 FDCPD 对细胞迁移均无影响，但未稀释时会导致成骨条件下的细胞活力下降。相反，VS20F 的洗脱液对干细胞分化有积极作用。ALP活性和ARS测定证实了这一结果。此外，在 hDPSCs 中还观察到 RUNX2、DMP-1 和 DSPP 等特定基因标记的上调，尤其是用 VS20F 处理时：本研究中测试的实验性 FDCPC 在成骨环境中具有剂量依赖性的矿化促进能力。FDCPC-VS20F似乎是最有前途的实验材料，适合开发具有成骨和生物活性特性的牙髓覆盖材料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Dental Materials 工程技术-材料科学：生物材料

CiteScore

9.80

自引率

10.00%

发文量

290

审稿时长

67 days

期刊介绍： Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.