Inflammatory and adhesion profile of gingival fibroblasts to lithium disilicate ceramic surfaces

IF 4.6 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Dental Materials Pub Date : 2024-11-01 DOI:10.1016/j.dental.2024.09.014

Jéssica Ferraz Carvalho Lima , Filipe Milazzo Santos , Taís Browne de Miranda , Guilherme Gama Ramos , Denise Carleto Andia , Adriano F. Lima , Danilo Lazzari Ciotti

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Abstract

Objectives

Lithium disilicate (LS) ceramic emerges as a compelling option for customized implant abutments. However, ensuring its safety and reliability requires clarification on key aspects, notably its impact on inflammation and potential for cell adhesion. This study delves into these considerations, examining the influence of LS ceramic on cytokine release and the transcriptional profile of human gingival fibroblasts (hGFs) in direct contact with various LS surfaces.

Methods

hGFs were cultured on LS disks featuring three distinct surfaces (unpolished, polished, and polished glaze), while titanium disks served as reference material and cells cultured directly on plates as controls. The surface of the disks was analyzed using a scanning electron microscope. The cell metabolism was analyzed by MTT test, cytokine release by MAGPIX and the expression of genes related to cell adhesion was evaluated by qPCR.

Results

The disks exhibited similar topography with smooth surfaces, except for the unpolished LS disks, which had an irregular surface. Contact with LS surfaces did not substantially reduce cell metabolism. Moreover, it generally decreased cytokine release compared to controls, particularly pro-inflammatory mediators like IL-1β, IL-6, and TNF-α. Significantly increased expression of genes related to cell adhesion to LS was observed, comparable to titanium, the gold standard material for implant abutments.

Significance: This study unveils that LS ceramic not only fails to trigger pro-inflammatory cytokine release, but also significantly enhances gene expression associated with cell adhesion. These mechanisms are closely linked to gene pathways such as PTK2, SRC, MAPK1, and transcription factors ELK-1 and MYC. In summary, the findings underscore LS ceramic's potential as a biocompatible material for implant abutments, shedding light on its favorable inflammatory response and enhanced cell adhesion properties.

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牙龈成纤维细胞对二硅酸锂陶瓷表面的炎症和粘附情况。

目的：二硅酸锂（LS）陶瓷作为定制种植基台的一种引人注目的选择。然而，要确保其安全性和可靠性，就必须澄清一些关键问题，特别是其对炎症的影响和细胞粘附的可能性。方法：在具有三种不同表面（未抛光、抛光和抛光釉面）的 LS 盘上培养 hGF，钛盘作为参考材料，细胞直接在平板上培养作为对照。使用扫描电子显微镜分析磁盘表面。用 MTT 测试分析了细胞代谢情况，用 MAGPIX 分析了细胞因子的释放情况，用 qPCR 评估了与细胞粘附有关的基因的表达情况：结果：除未抛光的 LS 盘表面不规则外，其他 LS 盘表面光滑，形貌相似。与 LS 表面接触并不会显著降低细胞的新陈代谢。此外，与对照组相比，细胞因子的释放量普遍减少，尤其是IL-1β、IL-6和TNF-α等促炎介质。观察到与细胞粘附在 LS 上相关的基因表达显著增加，与种植基台的金标准材料钛相当：本研究揭示了 LS 陶瓷不仅不会引发促炎细胞因子的释放，而且还能显著增强与细胞粘附相关的基因表达。这些机制与 PTK2、SRC、MAPK1 以及转录因子 ELK-1 和 MYC 等基因通路密切相关。总之，研究结果强调了 LS 陶瓷作为种植基台生物相容性材料的潜力，揭示了其有利的炎症反应和增强的细胞粘附特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Dental Materials 工程技术-材料科学：生物材料

CiteScore

9.80

自引率

10.00%

发文量

290

审稿时长

67 days

期刊介绍： Dental Materials publishes original research, review articles, and short communications. Academy of Dental Materials members click here to register for free access to Dental Materials online. The principal aim of Dental Materials is to promote rapid communication of scientific information between academia, industry, and the dental practitioner. Original Manuscripts on clinical and laboratory research of basic and applied character which focus on the properties or performance of dental materials or the reaction of host tissues to materials are given priority publication. Other acceptable topics include application technology in clinical dentistry and dental laboratory technology. Comprehensive reviews and editorial commentaries on pertinent subjects will be considered.