Structural studies of β-glucosidase from the thermophilic bacterium Caldicellulosiruptor saccharolyticus.

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS Acta Crystallographica. Section D, Structural Biology Pub Date : 2024-10-01 DOI:10.1107/S2059798324009252

Anastasia I Sotiropoulou, Dimitris G Hatzinikolaou, Evangelia D Chrysina

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Abstract

β-Glucosidase from the thermophilic bacterium Caldicellulosiruptor saccharolyticus (Bgl1) has been denoted as having an attractive catalytic profile for various industrial applications. Bgl1 catalyses the final step of in the decomposition of cellulose, an unbranched glucose polymer that has attracted the attention of researchers in recent years as it is the most abundant renewable source of reduced carbon in the biosphere. With the aim of enhancing the thermostability of Bgl1 for a broad spectrum of biotechnological processes, it has been subjected to structural studies. Crystal structures of Bgl1 and its complex with glucose were determined at 1.47 and 1.95 Å resolution, respectively. Bgl1 is a member of glycosyl hydrolase family 1 (GH1 superfamily, EC 3.2.1.21) and the results showed that the 3D structure of Bgl1 follows the overall architecture of the GH1 family, with a classical (β/α)₈ TIM-barrel fold. Comparisons of Bgl1 with sequence or structural homologues of β-glucosidase reveal quite similar structures but also unique structural features in Bgl1 with plausible functional roles.

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嗜热细菌钙纤维糖苷酶β-葡萄糖苷酶的结构研究。

嗜热细菌钙纤维糖苷酶（Caldicellulosiruptor saccharolyticus，Bgl1）的β-葡萄糖苷酶被认为在各种工业应用中具有诱人的催化特性。Bgl1 催化纤维素分解的最后一步，纤维素是一种未支链的葡萄糖聚合物，是生物圈中最丰富的可再生还原碳源，因此近年来引起了研究人员的关注。为了提高 Bgl1 在广泛的生物技术过程中的耐热性，我们对其进行了结构研究。分别以 1.47 和 1.95 Å 的分辨率测定了 Bgl1 及其与葡萄糖的复合物的晶体结构。Bgl1 是糖基水解酶家族 1（GH1 超家族，EC 3.2.1.21）的成员，研究结果表明 Bgl1 的三维结构遵循 GH1 家族的整体结构，具有经典的 (β/α)8 TIM 桶折叠。将 Bgl1 与 β-葡萄糖苷酶的序列或结构同源物进行比较发现，Bgl1 的结构十分相似，但也有独特的结构特征，并具有可信的功能作用。

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来源期刊

Acta Crystallographica. Section D, Structural Biology BIOCHEMICAL RESEARCH METHODSBIOCHEMISTRY &-BIOCHEMISTRY & MOLECULAR BIOLOGY

CiteScore

4.50

自引率

13.60%

发文量

216

期刊介绍： Acta Crystallographica Section D welcomes the submission of articles covering any aspect of structural biology, with a particular emphasis on the structures of biological macromolecules or the methods used to determine them. Reports on new structures of biological importance may address the smallest macromolecules to the largest complex molecular machines. These structures may have been determined using any structural biology technique including crystallography, NMR, cryoEM and/or other techniques. The key criterion is that such articles must present significant new insights into biological, chemical or medical sciences. The inclusion of complementary data that support the conclusions drawn from the structural studies (such as binding studies, mass spectrometry, enzyme assays, or analysis of mutants or other modified forms of biological macromolecule) is encouraged. Methods articles may include new approaches to any aspect of biological structure determination or structure analysis but will only be accepted where they focus on new methods that are demonstrated to be of general applicability and importance to structural biology. Articles describing particularly difficult problems in structural biology are also welcomed, if the analysis would provide useful insights to others facing similar problems.

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