Correlation of Clinical Severity of Trachomatous Inflammation and Chlamydia trachomatis Bacterial Load.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-10-01 DOI:10.4269/ajtmh.24-0319

Louisa Lu, Ali Fauci, Quintin R Richardson, Hamidah Mahmud, John M Nesemann, Solomon Aragie, Dagnachew Hailu, Adane Dagnew, Ambahun Chernet, Zerihun Tadesse, Taye Zeru, Isabel J B Thompson, Dionna M Wittberg, Scott D Nash, Thomas M Lietman, Jeremy D Keenan

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Abstract

The purpose of this study was to investigate the correlation between bacterial load of Chlamydia trachomatis as measured from quantitative polymerase chain reaction (qPCR) and the relative clinical severity of trachomatous inflammation. Individuals with trachoma from rural communities in Ethiopia had photographs taken as well as swabs obtained of the upper tarsal conjunctivas. Conjunctival swabs were processed with PCR assay, which provided quantitative results of ocular chlamydial load. A series of 125 conjunctival photographs were ranked from least to most severe according to clinical severity for follicular and papillary conjunctivitis. Higher intensity rankings of trachomatous inflammation were associated with higher chlamydial load for both follicular inflammation (Spearman's ρ = 0.43; P <0.001) and papillary inflammation (Spearman's ρ = 0.50; P <0.001). Rankings of trachomatous inflammation may be a clinically meaningful indicator of trachoma.

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沙眼炎症的临床严重程度与沙眼衣原体细菌量的相关性

本研究旨在探讨定量聚合酶链式反应（qPCR）测定的沙眼衣原体细菌量与沙眼炎症临床严重程度之间的相关性。研究人员为埃塞俄比亚农村社区的沙眼患者拍摄了照片，并采集了跗骨结膜上部的拭子。结膜拭子经过 PCR 检测处理，可提供眼部衣原体载量的定量结果。根据滤泡性结膜炎和乳头状结膜炎的临床严重程度，对 125 张结膜照片进行了从轻到重的排序。在滤泡性结膜炎和乳头状结膜炎中，沙眼炎症程度越严重，衣原体载量越高（Spearman's ρ = 0.43; P.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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