Titanium surfaces loaded with puerarin and exosomes derived from adipose stem cells promote the proliferation and differentiation of pre-osteoblasts.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-10-02 DOI:10.4012/dmj.2024-066

Yan Wang, Changlong Zhou, Qianqian Guo, Bin Chen, Jia Luo, Yimin Lv

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Abstract

The study is to evaluate the effects of collagen/hyaluronic acid coating with or without puerarin and exosomes (Exos) derived from adipose stem cells (ADSCs-Exos) on pre-osteoblast proliferation and differentiation on the surface of titanium materials. Titanium materials with different coatings were prepared by layer-by-layer technique, evaluating the surface characterization. Cell functions were assessed by cell biology experiments. Related genes and proteins were assessed by RT-qPCR and Western blot. Puerarin or ADSCs-Exos coating had better effects on promoting the adhesion, proliferation and differentiation of pre-osteoblasts, and the strongest effect was found after their co-coatings, manifesting as the up-regulations of alkaline phosphatase (ALP) activity, collagen type I alpha 1 (Col1a1), runt-related transcription factor 2 (Runx2), osterix and activating transcription factor-2 (ATF-2). Levels of phosphorylated-P38 (p-P38) and p-ATF-2 were up-regulated in pre-osteoblasts grown on puerarin and ADSCs-Exos-loaded titanium surfaces. Titanium surfaces loaded with puerarin and ADSCs-Exos promotes the proliferation and differentiation of pre-osteoblasts.

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加载了葛根素和脂肪干细胞外泌体的钛表面能促进前成骨细胞的增殖和分化。

本研究旨在评估添加或不添加葛根素的胶原蛋白/透明质酸涂层和脂肪干细胞衍生的外泌体（Exos）（ADSCs-Exos）对钛材料表面前成骨细胞增殖和分化的影响。通过逐层技术制备了具有不同涂层的钛材料，并对其表面特征进行了评估。细胞功能通过细胞生物学实验进行评估。相关基因和蛋白质通过 RT-qPCR 和 Western 印迹进行评估。Puerarin或ADSCs-Exos涂层对促进前成骨细胞的粘附、增殖和分化有更好的效果，它们共同涂层后的效果最强，表现为碱性磷酸酶（ALP）活性、Ⅰ型α1胶原（Col1a1）、Runt相关转录因子2（Runx2）、sterix和活化转录因子-2（ATF-2）的上调。在葛根素和 ADSCs-Exos 负载的钛表面生长的前成骨细胞中，磷酸化-P38（p-P38）和 p-ATF-2 的水平上调。负载葛根素和 ADSCs-Exos 的钛表面可促进前成骨细胞的增殖和分化。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.