Metals that are used to reconstruct skeletal structures often interfere with magnetic resonance imaging (MRI) owing to differences in magnetic susceptibility; consequently, metals with lower magnetic susceptibilities need to be developed for use in implant devices. Herein, we investigated the corrosion properties of the Zr-14Nb-5Ta-1Mo alloy, which exhibits low magnetic susceptibility and excellent mechanical properties. The pitting potential of Zr-14Nb-5Ta-1Mo was higher than that of pure Zr. The passive current density of Zr-14Nb-5Ta-1Mo also higher than that of pure Zr, which is ascribable to slow reconstruction of the initial passive film associated with the presence of Nb and Ta. XPS revealed that the passive film is enriched with Nb and Ta. Therefore, while the Zr-14Nb-5Ta-1Mo alloy exhibited a high initial passive current density in simulated body fluid, it formed a stable passive film that suppressed localized corrosion. Zr-14Nb-5Ta-1Mo is therefore a prospective implant-material alloy candidate.
由于磁感应强度的差异,用于重建骨骼结构的金属往往会干扰磁共振成像(MRI);因此,需要开发磁感应强度较低的金属,用于植入装置。在此,我们研究了具有低磁感应强度和优异机械性能的 Zr-14Nb-5Ta-1Mo 合金的腐蚀特性。Zr-14Nb-5Ta-1Mo 的点蚀电位高于纯 Zr。Zr-14Nb-5Ta-1Mo 的无源电流密度也比纯 Zr 高,这可能与 Nb 和 Ta 的存在导致初始无源膜重建缓慢有关。XPS 显示,被动膜富含 Nb 和 Ta。因此,虽然 Zr-14Nb-5Ta-1Mo 合金在模拟体液中表现出较高的初始无源电流密度,但它形成的稳定无源膜抑制了局部腐蚀。因此,Zr-14Nb-5Ta-1Mo 是一种很有前景的植入材料合金。
{"title":"Corrosion behavior of Zr-14Nb-5Ta-1Mo alloy in simulated body fluid.","authors":"Tomoyo Manaka, Yusuke Tsutsumi, Maki Ashida, Peng Chen, Takao Hanawa","doi":"10.4012/dmj.2024-158","DOIUrl":"https://doi.org/10.4012/dmj.2024-158","url":null,"abstract":"<p><p>Metals that are used to reconstruct skeletal structures often interfere with magnetic resonance imaging (MRI) owing to differences in magnetic susceptibility; consequently, metals with lower magnetic susceptibilities need to be developed for use in implant devices. Herein, we investigated the corrosion properties of the Zr-14Nb-5Ta-1Mo alloy, which exhibits low magnetic susceptibility and excellent mechanical properties. The pitting potential of Zr-14Nb-5Ta-1Mo was higher than that of pure Zr. The passive current density of Zr-14Nb-5Ta-1Mo also higher than that of pure Zr, which is ascribable to slow reconstruction of the initial passive film associated with the presence of Nb and Ta. XPS revealed that the passive film is enriched with Nb and Ta. Therefore, while the Zr-14Nb-5Ta-1Mo alloy exhibited a high initial passive current density in simulated body fluid, it formed a stable passive film that suppressed localized corrosion. Zr-14Nb-5Ta-1Mo is therefore a prospective implant-material alloy candidate.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to investigate the wear and microhardness of luting agents and computer-aided design-computer-aided manufacturing (CAD-CAM) resin blocks, and to compare the wear characteristics of resin-based luting agents used in bonded specimens of CAD-CAM resin blocks. After three-body wear test, the amount of wear and Vickers hardness were measured and Pearson's correlation coefficient was calculated. The lowest amount of wear and the highest Vickers hardness values were obtained for Estecem II. Filler loss was observed in five composite resin cements. A strong negative correlation was observed in the luting agents between the amount of wear and Vickers hardness value (r=-0.874, p<0.0001). Additionally, a strong positive correlation was observed between the amount of wear of the bonded specimen and amount of luting agent used alone (r=0.943, p<0.0001). To conclude, the wear resistance of the luting agent used in the bonded specimen was significantly influenced by the bonding between the filler and matrix resin.
本研究旨在探究敷料和计算机辅助设计-计算机辅助制造(CAD-CAM)树脂块的磨损和显微硬度,并比较用于 CAD-CAM 树脂块粘结试样的树脂基敷料的磨损特性。三体磨损试验后,测量了磨损量和维氏硬度,并计算了皮尔逊相关系数。Estecem II 的磨损量最小,维氏硬度值最高。在五种复合树脂水门汀中观察到了填料流失。在粘接剂中,磨损量和维氏硬度值之间呈强烈的负相关(r=-0.874,p<0.05)。
{"title":"Wear characteristics of resin-based luting agents used in the bonded CAD-CAM resin blocks.","authors":"Hiroto Takenaka, Keiichi Yoshida, Takashi Sawase","doi":"10.4012/dmj.2024-137","DOIUrl":"https://doi.org/10.4012/dmj.2024-137","url":null,"abstract":"<p><p>This study aimed to investigate the wear and microhardness of luting agents and computer-aided design-computer-aided manufacturing (CAD-CAM) resin blocks, and to compare the wear characteristics of resin-based luting agents used in bonded specimens of CAD-CAM resin blocks. After three-body wear test, the amount of wear and Vickers hardness were measured and Pearson's correlation coefficient was calculated. The lowest amount of wear and the highest Vickers hardness values were obtained for Estecem II. Filler loss was observed in five composite resin cements. A strong negative correlation was observed in the luting agents between the amount of wear and Vickers hardness value (r=-0.874, p<0.0001). Additionally, a strong positive correlation was observed between the amount of wear of the bonded specimen and amount of luting agent used alone (r=0.943, p<0.0001). To conclude, the wear resistance of the luting agent used in the bonded specimen was significantly influenced by the bonding between the filler and matrix resin.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Merve Gürses, Bahar Inan, Nevin Cobanoglu, Atiye Tugba Kuzgun Türkmen
This study evaluates the 5-year clinical performance of Class II restorations performed with different bulk-fill restorative materials. In the study, Class II restorations performed with Tetric Bulk-Fill (TBF), Filtek Bulk-Fill (FBF), and Equia Forte Fil (EF) were evaluated. One hundred-nineteen restorations were included in the study. Restorations were assessed during the 6th month, 1st, 2nd, and 5th year. Cochran Q, Pearson chi-square, and Fisher-Freeman-Halton tests were used for statistical analysis. In the 5th year, significant differences were observed in terms of retention, color match, marginal adaptation, marginal discoloration, surface texture, and anatomical form in all materials. There was a significant difference between EF and bulk-fill composites only in terms of retention and anatomical form. EF was significantly less successful than bulk-fill composites with regard to retention and anatomical form, but bulk-fill composites have shown similar clinical performance. EF cannot be an alternative to bulk-fill composites for Class II restorations.
{"title":"Five-year clinical follow-up of bulk-fill restorative materials in class II restorations.","authors":"Merve Gürses, Bahar Inan, Nevin Cobanoglu, Atiye Tugba Kuzgun Türkmen","doi":"10.4012/dmj.2024-132","DOIUrl":"https://doi.org/10.4012/dmj.2024-132","url":null,"abstract":"<p><p>This study evaluates the 5-year clinical performance of Class II restorations performed with different bulk-fill restorative materials. In the study, Class II restorations performed with Tetric Bulk-Fill (TBF), Filtek Bulk-Fill (FBF), and Equia Forte Fil (EF) were evaluated. One hundred-nineteen restorations were included in the study. Restorations were assessed during the 6th month, 1st, 2nd, and 5th year. Cochran Q, Pearson chi-square, and Fisher-Freeman-Halton tests were used for statistical analysis. In the 5th year, significant differences were observed in terms of retention, color match, marginal adaptation, marginal discoloration, surface texture, and anatomical form in all materials. There was a significant difference between EF and bulk-fill composites only in terms of retention and anatomical form. EF was significantly less successful than bulk-fill composites with regard to retention and anatomical form, but bulk-fill composites have shown similar clinical performance. EF cannot be an alternative to bulk-fill composites for Class II restorations.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Hoda Mohamed Elnawawy, Muralithran Govindan Kutty, Noor Azlin Yahya, Noor Hayaty Abu Kasim, Paul Roy Cooper, Josette Camilleri, Hany Mohamed Aly Ahmed
This study compared the chemical and physical properties of an experimental radiopaque white Portland cement (REPC) with reduced particle size to ProRoot white mineral trioxide aggregate (WMTA). The particle size distribution of experimental Portland cement (EPC) was examined, and then nano-zirconium oxide (nano-ZrO) was added to produce REPC. Chemical analysis, initial setting time, pH values, and push-out bond strength were evaluated. Results showed that REPC had smallest particle size (354.5±26.45 nm), while PC had the largest (1,309.67±60.54 nm) (p<0.05). Differences in chemical composition were observed. REPC exhibited shorter setting time (32.7±0.58 min) compared to WMTA (131.67±2.89 min) and PC (163.33±2.89 min) (p<0.05). All groups showed alkaline pH (p<0.05). REPC demonstrated the highest push-out bond strength (22.24±4.33 MPa) compared with WMTA (15.53±3.26 MPa) and PC (16.8±5.43 MPa) (p<0.05). This cost-effective PC formulation reduced the setting time and increased the push-out bond strength while maintaining the alkaline properties of the original cements.
本研究比较了粒径减小的不透射线白色硅酸盐水泥(REPC)与 ProRoot 三氧化二铝白色矿物骨料(WMTA)的化学和物理特性。研究人员检测了实验波特兰水泥(EPC)的粒度分布,然后添加纳米氧化锆(nano-ZrO)生产 REPC。对化学分析、初凝时间、pH 值和推出粘接强度进行了评估。结果表明,REPC 的粒径最小(354.5±26.45 nm),而 PC 的粒径最大(1,309.67±60.54 nm)(p<0.05)。
{"title":"Chemical and physical properties of radiopaque Portland cement formulation with reduced particle size.","authors":"Hoda Mohamed Elnawawy, Muralithran Govindan Kutty, Noor Azlin Yahya, Noor Hayaty Abu Kasim, Paul Roy Cooper, Josette Camilleri, Hany Mohamed Aly Ahmed","doi":"10.4012/dmj.2024-015","DOIUrl":"https://doi.org/10.4012/dmj.2024-015","url":null,"abstract":"<p><p>This study compared the chemical and physical properties of an experimental radiopaque white Portland cement (REPC) with reduced particle size to ProRoot white mineral trioxide aggregate (WMTA). The particle size distribution of experimental Portland cement (EPC) was examined, and then nano-zirconium oxide (nano-ZrO) was added to produce REPC. Chemical analysis, initial setting time, pH values, and push-out bond strength were evaluated. Results showed that REPC had smallest particle size (354.5±26.45 nm), while PC had the largest (1,309.67±60.54 nm) (p<0.05). Differences in chemical composition were observed. REPC exhibited shorter setting time (32.7±0.58 min) compared to WMTA (131.67±2.89 min) and PC (163.33±2.89 min) (p<0.05). All groups showed alkaline pH (p<0.05). REPC demonstrated the highest push-out bond strength (22.24±4.33 MPa) compared with WMTA (15.53±3.26 MPa) and PC (16.8±5.43 MPa) (p<0.05). This cost-effective PC formulation reduced the setting time and increased the push-out bond strength while maintaining the alkaline properties of the original cements.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142281771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Miki Hori, Makoto Jincho, Tadasuke Hori, Hironao Sekine, Akiko Kato, Ken Miyazawa, Tatsushi Kawai
This project aimed to develop an artificial intelligence program tailored for cephalometric images. The program employs a convolutional neural network with 6 convolutional layers and 2 affine layers. It identifies 18 key points on the skull to compute various angles essential for diagnosis. Utilizing a custom-built desktop computer with a moderately priced graphics processing unit, cephalogram images were resized to 800×800 pixels. Training data comprised 833 images, augmented 100 times; an additional 179 images were used for testing. Due to the complexity of training with full-size images, training was divided into two steps. The first step reduced images to 128×128 pixels, recognizing all 18 points. In the second step, 100×100 pixels blocks were extracted from original images for individual point training. The program then measured six angles, achieving an average error of 3.1 pixels for the 18 points, with SNA and SNB angles showing an average difference of less than 1°.
{"title":"Automatic point detection on cephalograms using convolutional neural networks: A two-step method.","authors":"Miki Hori, Makoto Jincho, Tadasuke Hori, Hironao Sekine, Akiko Kato, Ken Miyazawa, Tatsushi Kawai","doi":"10.4012/dmj.2024-052","DOIUrl":"https://doi.org/10.4012/dmj.2024-052","url":null,"abstract":"<p><p>This project aimed to develop an artificial intelligence program tailored for cephalometric images. The program employs a convolutional neural network with 6 convolutional layers and 2 affine layers. It identifies 18 key points on the skull to compute various angles essential for diagnosis. Utilizing a custom-built desktop computer with a moderately priced graphics processing unit, cephalogram images were resized to 800×800 pixels. Training data comprised 833 images, augmented 100 times; an additional 179 images were used for testing. Due to the complexity of training with full-size images, training was divided into two steps. The first step reduced images to 128×128 pixels, recognizing all 18 points. In the second step, 100×100 pixels blocks were extracted from original images for individual point training. The program then measured six angles, achieving an average error of 3.1 pixels for the 18 points, with SNA and SNB angles showing an average difference of less than 1°.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Premixed calcium silicate-based materials have recently been developed and are recommended for a wide range of endodontic procedures, including vital pulp therapy. This study investigated the in vitro biocompatibility and pro-mineralization effect and in vivo reparative dentin formation of EndoSequence Root Repair Material, EndoSequence BCRRM, Bio-C Repair, and Well-pulp PT. Both fresh and set extracts had no detrimental effect on the growth of human dental pulp stem cells. The fresh extracts had a higher calcium concentration than the set extracts and induced considerably greater mineralized nodule formation. EndoSequence Root Repair Material had the longest setting time, whereas Bio-C Repair had the shortest. When these materials were applied to exposed rat molar pulps, mineralized tissue deposition was found at the exposure sites after 2 weeks. These results indicate that the premixed calcium silicate-based materials tested could have positive benefits for direct pulp capping procedures.
{"title":"Biocompatibility and pro-mineralization effects of premixed calcium silicate-based materials on human dental pulp stem cells: An in vitro and in vivo study.","authors":"Nyein Chan Ko, Sonoko Noda, Yamato Okada, Kento Tazawa, Nobuyuki Kawashima, Takashi Okiji","doi":"10.4012/dmj.2024-121","DOIUrl":"https://doi.org/10.4012/dmj.2024-121","url":null,"abstract":"<p><p>Premixed calcium silicate-based materials have recently been developed and are recommended for a wide range of endodontic procedures, including vital pulp therapy. This study investigated the in vitro biocompatibility and pro-mineralization effect and in vivo reparative dentin formation of EndoSequence Root Repair Material, EndoSequence BCRRM, Bio-C Repair, and Well-pulp PT. Both fresh and set extracts had no detrimental effect on the growth of human dental pulp stem cells. The fresh extracts had a higher calcium concentration than the set extracts and induced considerably greater mineralized nodule formation. EndoSequence Root Repair Material had the longest setting time, whereas Bio-C Repair had the shortest. When these materials were applied to exposed rat molar pulps, mineralized tissue deposition was found at the exposure sites after 2 weeks. These results indicate that the premixed calcium silicate-based materials tested could have positive benefits for direct pulp capping procedures.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142132095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oskar Bunz, Max Diekamp, Mozhgan Bizhang, Holger Testrich, Andree Piwowarczyk
This study investigates the surface quality and bacterial adhesion properties of various dental materials, including indirect composites, veneering composites, direct composites, polyether ether ketone (PEEK), and two millable polymethyl methacrylate (PMMA). Material specimens were processed following manufacturer instructions, initially evaluated for surface roughness and Streptococcus sanguinis (S. sanguinis) adhesion. Subsequently, toothbrushing simulation was employed to simulate aging, and changes in material surfaces were assessed via roughness measurements and bacterial adhesion testing. Prior to simulated aging, direct and indirect composites exhibited the lowest roughness values. However, after the simulated toothbrushing, veneering composites displayed the highest roughness levels. Both PMMA materials demonstrated the highest S. sanguinis adhesion levels, both before and after artificial aging. Interestingly, the indirect composite material showed a reduction in bacterial adhesion following toothbrushing simulation. Surprisingly, this study did not reveal a clear correlation between roughness and bacterial adhesion.
本研究调查了各种牙科材料的表面质量和细菌粘附特性,包括间接复合材料、贴面复合材料、直接复合材料、聚醚醚酮 (PEEK) 和两种可磨聚甲基丙烯酸甲酯 (PMMA)。材料试样按照制造商的说明进行加工,首先评估表面粗糙度和血清链球菌(S. sanguinis)粘附性。随后,采用模拟刷牙的方法来模拟老化,并通过粗糙度测量和细菌粘附测试来评估材料表面的变化。在模拟老化之前,直接和间接复合材料的粗糙度值最低。然而,在模拟刷牙后,贴面复合材料显示出最高的粗糙度水平。无论是在人工老化之前还是之后,两种 PMMA 材料都显示出最高的 S. sanguinis 粘附水平。有趣的是,在模拟刷牙后,间接复合材料的细菌附着力有所下降。令人惊讶的是,这项研究并未发现粗糙度与细菌附着力之间存在明显的相关性。
{"title":"Surface roughness associated with bacterial adhesion on dental resin-based materials.","authors":"Oskar Bunz, Max Diekamp, Mozhgan Bizhang, Holger Testrich, Andree Piwowarczyk","doi":"10.4012/dmj.2023-234","DOIUrl":"https://doi.org/10.4012/dmj.2023-234","url":null,"abstract":"<p><p>This study investigates the surface quality and bacterial adhesion properties of various dental materials, including indirect composites, veneering composites, direct composites, polyether ether ketone (PEEK), and two millable polymethyl methacrylate (PMMA). Material specimens were processed following manufacturer instructions, initially evaluated for surface roughness and Streptococcus sanguinis (S. sanguinis) adhesion. Subsequently, toothbrushing simulation was employed to simulate aging, and changes in material surfaces were assessed via roughness measurements and bacterial adhesion testing. Prior to simulated aging, direct and indirect composites exhibited the lowest roughness values. However, after the simulated toothbrushing, veneering composites displayed the highest roughness levels. Both PMMA materials demonstrated the highest S. sanguinis adhesion levels, both before and after artificial aging. Interestingly, the indirect composite material showed a reduction in bacterial adhesion following toothbrushing simulation. Surprisingly, this study did not reveal a clear correlation between roughness and bacterial adhesion.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to investigate the effect of acidulated phosphate fluoride (APF) application on filler-free polymethyl methacrylate (PMMA)-based resin blocks for computer-aided design/computer-aided manufacturing (CAD-CAM), focusing on their use in pediatric crowns. Three types of PMMA-based blocks for CAD-CAM were evaluated, and a composite resin block for CAD-CAM was used as a control. Statistical analysis (p<0.05) of the data revealed that all PMMA-based blocks showed significantly higher gloss levels than the composite resin blocks. Two PMMA-based blocks also demonstrated significantly lower Ra and Sa values. SEM images showed no irregular changes in the surface properties of the PMMA-based blocks compared to those of the composite resin block. These results are significant in meeting the increasing demand for esthetic restorative treatments in pediatrics, where APF is commonly used for caries prevention. PMMA-based resin blocks for CAD-CAM are an effective alternative to prevent esthetic degradation from gloss reduction and plaque accumulation.
{"title":"Changes in the surface texture of pre-polymerized acrylic resin pediatric crowns following acidulated phosphate fluoride application.","authors":"Keiya Nanri, Hiroyasu Koizumi, Haruto Hiraba, Takashi Kikuiri, Takayuki Yoneyama","doi":"10.4012/dmj.2024-083","DOIUrl":"https://doi.org/10.4012/dmj.2024-083","url":null,"abstract":"<p><p>This study aimed to investigate the effect of acidulated phosphate fluoride (APF) application on filler-free polymethyl methacrylate (PMMA)-based resin blocks for computer-aided design/computer-aided manufacturing (CAD-CAM), focusing on their use in pediatric crowns. Three types of PMMA-based blocks for CAD-CAM were evaluated, and a composite resin block for CAD-CAM was used as a control. Statistical analysis (p<0.05) of the data revealed that all PMMA-based blocks showed significantly higher gloss levels than the composite resin blocks. Two PMMA-based blocks also demonstrated significantly lower Ra and Sa values. SEM images showed no irregular changes in the surface properties of the PMMA-based blocks compared to those of the composite resin block. These results are significant in meeting the increasing demand for esthetic restorative treatments in pediatrics, where APF is commonly used for caries prevention. PMMA-based resin blocks for CAD-CAM are an effective alternative to prevent esthetic degradation from gloss reduction and plaque accumulation.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Karina Erda Saninggar, Fumika Abe, Ayana Nakano, Koichi Kato
Bone tissue engineering using biodegradable porous scaffolds is a promising approach for restoring oral and maxillofacial bone defects. Recently, attempts have been made to incorporate proteins such as growth factors to create bioactive scaffolds that can engage cells to promote tissue formation. Collagen-based scaffolds containing bone morphogenetic protein-2 (BMP2) have been studied for bone formation. However, controlling the initial burst of BMP2 remains difficult. Here we designed a functional chimeric protein composed of BMP2 and a collagen-binding domain (CBD), specifically the A3 domain of von Willebrand factor, to sustain BMP2 release from collagen-based scaffolds. Based on the results of computer-based structural prediction, we prepared a chimeric protein consisting of CBD and BMP2 in this order with a peptide tag for affinity purification. The chimeric protein had a collagen-binding capacity and enhanced osteogenic differentiation of human mesenchymal stem cells. These results are consistent with insights from in silico structural prediction.
{"title":"Collagen-binding bone morphogenetic protein-2 designed for use in bone tissue engineering.","authors":"Karina Erda Saninggar, Fumika Abe, Ayana Nakano, Koichi Kato","doi":"10.4012/dmj.2024-138","DOIUrl":"https://doi.org/10.4012/dmj.2024-138","url":null,"abstract":"<p><p>Bone tissue engineering using biodegradable porous scaffolds is a promising approach for restoring oral and maxillofacial bone defects. Recently, attempts have been made to incorporate proteins such as growth factors to create bioactive scaffolds that can engage cells to promote tissue formation. Collagen-based scaffolds containing bone morphogenetic protein-2 (BMP2) have been studied for bone formation. However, controlling the initial burst of BMP2 remains difficult. Here we designed a functional chimeric protein composed of BMP2 and a collagen-binding domain (CBD), specifically the A3 domain of von Willebrand factor, to sustain BMP2 release from collagen-based scaffolds. Based on the results of computer-based structural prediction, we prepared a chimeric protein consisting of CBD and BMP2 in this order with a peptide tag for affinity purification. The chimeric protein had a collagen-binding capacity and enhanced osteogenic differentiation of human mesenchymal stem cells. These results are consistent with insights from in silico structural prediction.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142105155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Widowati Siswomihardjo, Ika Dewi Ana, Retno Ardhani
The use of biogenic calcium ions for the source of hydroxyapatite (HAp or HA) are very common and have been being explored extensively. However, it usually results high crystalline HA, due to high reaction and decomposition temperatures. In this study, strontium (Sr2+) doped HA from the golden apple snail shells (Pomacea canaliculate L) was successfully synthesized. It was indicated that Sr ions completely replaced calcium (Ca) ions, increased the lattice constant, and consecutively reduced HA crystallinity. Smaller crystal size and β-type carbonate (CO32-) ions substitution with Ca/P close to 1.67 molar ratio that mimic bone crystals were observed in Sr-doped HA, with significant increased rate of MC3T3-E1 cells viability and higher IC50 values. It was proven that Sr ions substitution resolved challenges on the use of biogenic sources for HA fabrication. Further in vivo study is needed to continue to valorise the results into real biomedical and clinical applications.
使用生物钙离子作为羟基磷灰石(HAp 或 HA)的来源非常普遍,并得到了广泛的探索。然而,由于反应和分解温度较高,通常会产生高结晶的 HA。本研究成功合成了掺杂锶(Sr2+)的金苹果螺壳(Pomacea canaliculate L)羟基磷灰石。结果表明,Sr 离子完全取代了钙(Ca)离子,增加了晶格常数,并连续降低了 HA 的结晶度。在掺 Sr 的 HA 中观察到晶体尺寸变小,β 型碳酸根(CO32-)离子取代 Ca/P 摩尔比接近 1.67,模拟骨晶体,MC3T3-E1 细胞存活率显著提高,IC50 值升高。事实证明,Sr 离子的替代解决了使用生物源制造 HA 的难题。要继续将这些结果应用于真正的生物医学和临床应用,还需要进一步的体内研究。
{"title":"Fabrication of strontium ions substituted hydroxyapatite from the shells of the golden apple snail (Pomacea canaliculate L) with enhanced osteoconductive and improved biological properties.","authors":"Widowati Siswomihardjo, Ika Dewi Ana, Retno Ardhani","doi":"10.4012/dmj.2023-246","DOIUrl":"https://doi.org/10.4012/dmj.2023-246","url":null,"abstract":"<p><p>The use of biogenic calcium ions for the source of hydroxyapatite (HAp or HA) are very common and have been being explored extensively. However, it usually results high crystalline HA, due to high reaction and decomposition temperatures. In this study, strontium (Sr<sup>2+</sup>) doped HA from the golden apple snail shells (Pomacea canaliculate L) was successfully synthesized. It was indicated that Sr ions completely replaced calcium (Ca) ions, increased the lattice constant, and consecutively reduced HA crystallinity. Smaller crystal size and β-type carbonate (CO<sub>3</sub><sup>2-</sup>) ions substitution with Ca/P close to 1.67 molar ratio that mimic bone crystals were observed in Sr-doped HA, with significant increased rate of MC3T3-E1 cells viability and higher IC<sub>50</sub> values. It was proven that Sr ions substitution resolved challenges on the use of biogenic sources for HA fabrication. Further in vivo study is needed to continue to valorise the results into real biomedical and clinical applications.</p>","PeriodicalId":11065,"journal":{"name":"Dental materials journal","volume":null,"pages":null},"PeriodicalIF":1.9,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142092517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}