Melanys Benitez;Pablo Zubiate;Abián B. Socorro;Ignacio R. Matías
{"title":"Revealing the Capability of an LMR Microfluidic Biosensor for Celiac Disease Diagnosis via Label-Free Detection of Antigliadin Antibodies","authors":"Melanys Benitez;Pablo Zubiate;Abián B. Socorro;Ignacio R. Matías","doi":"10.1109/LSENS.2024.3443461","DOIUrl":null,"url":null,"abstract":"Celiac disease (CD) is a chronic autoimmune disorder triggered by gluten consumption, which affects between 0.5% and 1% of the global population. Current diagnostic methods still require invasive pro-cedures, such as intestinal biopsy. Lossy mode resonance (LMR)-based sensors hold great potential for the development of reliable and user-friendly devices for diagnosing this condition. In this letter, an LMR planar microfluidic system is used to perform the label-free detection of different concentrations of antigliadin antibodies, one of the biomarkers of celiac disease, achieving a limit of detection of 1 µg/mL. The speci- ficity of the sensor to the target analyte is also proved, and the validation of the biofunctionalization process is comple- mented with an atomic force microscope analysis.","PeriodicalId":13014,"journal":{"name":"IEEE Sensors Letters","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"IEEE Sensors Letters","FirstCategoryId":"1085","ListUrlMain":"https://ieeexplore.ieee.org/document/10636190/","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0
Abstract
Celiac disease (CD) is a chronic autoimmune disorder triggered by gluten consumption, which affects between 0.5% and 1% of the global population. Current diagnostic methods still require invasive pro-cedures, such as intestinal biopsy. Lossy mode resonance (LMR)-based sensors hold great potential for the development of reliable and user-friendly devices for diagnosing this condition. In this letter, an LMR planar microfluidic system is used to perform the label-free detection of different concentrations of antigliadin antibodies, one of the biomarkers of celiac disease, achieving a limit of detection of 1 µg/mL. The speci- ficity of the sensor to the target analyte is also proved, and the validation of the biofunctionalization process is comple- mented with an atomic force microscope analysis.