Revealing the Capability of an LMR Microfluidic Biosensor for Celiac Disease Diagnosis via Label-Free Detection of Antigliadin Antibodies

Abstract

Celiac disease (CD) is a chronic autoimmune disorder triggered by gluten consumption, which affects between 0.5% and 1% of the global population. Current diagnostic methods still require invasive pro-cedures, such as intestinal biopsy. Lossy mode resonance (LMR)-based sensors hold great potential for the development of reliable and user-friendly devices for diagnosing this condition. In this letter, an LMR planar microfluidic system is used to perform the label-free detection of different concentrations of antigliadin antibodies, one of the biomarkers of celiac disease, achieving a limit of detection of 1 µg/mL. The speci- ficity of the sensor to the target analyte is also proved, and the validation of the biofunctionalization process is comple- mented with an atomic force microscope analysis.