NMR assignment of the conserved bacterial DNA replication protein DnaA domain IV

IF 0.8 4区 生物学 Q4 BIOPHYSICS Biomolecular NMR Assignments Pub Date : 2024-10-04 DOI:10.1007/s12104-024-10206-1
Alexander Nguyen Abrams, Geoff Kelly, Julia Hubbard
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Abstract

Chromosomal replication is a ubiquitous and essential cellular process. In bacteria, the master replication initiator DnaA plays a key role in promoting an open complex at the origin (oriC) and recruiting helicase in a tightly regulated process. The C-terminal domain IV specifically recognises consensus sequences of double-stranded DNA in oriC, termed DnaA-boxes, thereby facilitating the initial engagement of DnaA to oriC. Here, we report the 13Cβ and backbone 1H, 15N, and 13C chemical shift assignments of soluble DnaA domain IV from Bacillus subtilis at pH 7.6 and 298 K.

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保守细菌 DNA 复制蛋白 DnaA 结构域 IV 的核磁共振分配。
染色体复制是一个无处不在的重要细胞过程。在细菌中,主复制启动子 DnaA 在促进原点(oriC)开放复合物和招募螺旋酶的严格调控过程中发挥着关键作用。其 C 端结构域 IV 能特异性识别 oriC 中双链 DNA 的共识序列(称为 DnaA-boxes),从而促进 DnaA 与 oriC 的初始接合。在此,我们报告了枯草芽孢杆菌可溶性 DnaA 结构域 IV 在 pH 7.6 和 298 K 条件下的 13Cβ 和骨架 1H、15N 和 13C 化学位移赋值。
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来源期刊
Biomolecular NMR Assignments
Biomolecular NMR Assignments 生物-光谱学
CiteScore
1.70
自引率
11.10%
发文量
59
审稿时长
6-12 weeks
期刊介绍: Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.
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