Investigating the mechanisms of flucarbazone-sodium resistance in Bromus japonicus Thunb

IF 3.9 2区农林科学 Q1 AGRONOMY Plant and Soil Pub Date : 2024-10-04 DOI:10.1007/s11104-024-06967-2

Yuning Lan, Xinhui Xue, Hailan Cui, Ruolin Wang, Ying Sun, Hongjuan Huang, Shouhui Wei, Xiangju Li, Zhaofeng Huang

{"title":"Investigating the mechanisms of flucarbazone-sodium resistance in Bromus japonicus Thunb","authors":"Yuning Lan, Xinhui Xue, Hailan Cui, Ruolin Wang, Ying Sun, Hongjuan Huang, Shouhui Wei, Xiangju Li, Zhaofeng Huang","doi":"10.1007/s11104-024-06967-2","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Background</h3>Bromus japonicus Thunb. is a hazardous weed in wheat fields in China. One B. japonicus population, HB-1, which was collected in Hebei Province, survived field rate flucarbazone-sodium exposure.<h3 data-test=\"abstract-sub-heading\">Method</h3>To investigate the mechanism of flucarbazone-sodium resistance in the HB-1 population, the herbicide target als gene was isolated, and the in vitro ALS activity was tested. HPLC‒MS/MS was applied to detect flucarbazone-sodium absorption and residue level. Furthermore, RNA-seq and qRT‒PCR were applied to detect genes related to flucarbazone-sodium metabolism or flucarbazone-sodium and its metabolite transport.<h3 data-test=\"abstract-sub-heading\">Result</h3>The results indicated that in resistant population there were no mutations in the als gene sequences or differences in the IC50 of ALS compared with the sensitive population. In addition, als gene relative copy number and expression also showed no difference. However, significantly increased herbicide metabolism was found in the HB-1 population. In the end, four cytochrome P450 genes (C71D55b, C71A1, C71D55, T5H), three glutathione S-transferase genes (GSTZ, GSTU6b, GSTFB), and three ABC transporter genes (ABCC3, ABCF1, ABCG11) were selected and identified as potentially playing a role in flucarbazone-sodium resistance.<h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\n","PeriodicalId":20223,"journal":{"name":"Plant and Soil","volume":"47 1","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant and Soil","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s11104-024-06967-2","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Bromus japonicus Thunb. is a hazardous weed in wheat fields in China. One B. japonicus population, HB-1, which was collected in Hebei Province, survived field rate flucarbazone-sodium exposure.

Method

To investigate the mechanism of flucarbazone-sodium resistance in the HB-1 population, the herbicide target als gene was isolated, and the in vitro ALS activity was tested. HPLC‒MS/MS was applied to detect flucarbazone-sodium absorption and residue level. Furthermore, RNA-seq and qRT‒PCR were applied to detect genes related to flucarbazone-sodium metabolism or flucarbazone-sodium and its metabolite transport.

Result

The results indicated that in resistant population there were no mutations in the als gene sequences or differences in the IC₅₀ of ALS compared with the sensitive population. In addition, als gene relative copy number and expression also showed no difference. However, significantly increased herbicide metabolism was found in the HB-1 population. In the end, four cytochrome P450 genes (C71D55b, C71A1, C71D55, T5H), three glutathione S-transferase genes (GSTZ, GSTU6b, GSTFB), and three ABC transporter genes (ABCC3, ABCF1, ABCG11) were selected and identified as potentially playing a role in flucarbazone-sodium resistance.

Graphical Abstract

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

研究日本禾本科植物对氟巴唑钠的抗性机理

背景日本芒属（Bromus japonicus Thunb.方法为研究HB-1群体对氟草腙的抗性机理，分离了其除草剂靶标als基因，并检测了其体外ALS活性。采用HPLC-MS/MS检测氟嗪草酮钠的吸收和残留水平。结果表明，与敏感群体相比，抗性群体的als基因序列没有突变，ALS的IC50也没有差异。此外，als 基因的相对拷贝数和表达量也没有差异。然而，在 HB-1 群体中发现除草剂代谢明显增加。最后，研究人员筛选并确定了 4 个细胞色素 P450 基因（C71D55b、C71A1、C71D55、T5H）、3 个谷胱甘肽 S 转移酶基因（GSTZ、GSTU6b、GSTFB）和 3 个 ABC 转运体基因（ABCC3、ABCF1、ABCG11）可能在氟磺胺草醚抗性中起作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.