Increased CRHR1 expression on monocytes from patients with AA enables a pro-inflammatory response to corticotrophin-releasing hormone

IF 3.5 3区 医学 Q1 DERMATOLOGY Experimental Dermatology Pub Date : 2024-10-04 DOI:10.1111/exd.15182
Hong-Wei Guo, Hui-Jun Lai, Bo-Quan Long, Li-Xin Xu, Eddy Hsi Chun Wang, Jerry Shapiro, Kevin J. McElwee
{"title":"Increased CRHR1 expression on monocytes from patients with AA enables a pro-inflammatory response to corticotrophin-releasing hormone","authors":"Hong-Wei Guo,&nbsp;Hui-Jun Lai,&nbsp;Bo-Quan Long,&nbsp;Li-Xin Xu,&nbsp;Eddy Hsi Chun Wang,&nbsp;Jerry Shapiro,&nbsp;Kevin J. McElwee","doi":"10.1111/exd.15182","DOIUrl":null,"url":null,"abstract":"<p>Stress may play a key role in alopecia areata (AA), though the exact interactions of stress with AA remain undefined. Corticotropin-releasing hormone (CRH), the proximal regulator of the stress axis, has been recognized as an immunomodulatory factor in tissues and peripheral blood mononuclear cells (PBMCs). We used multicolour flow cytometry to identify receptor CRHR1 expression on PBMC subsets in AA patients (<i>n</i> = 54) and controls (<i>n</i> = 66). We found that CRHR1 was primarily expressed by circulating monocytes. CRHR1 expression on monocytes was enhanced in AA compared with controls (3.17% vs. 1.44%, <i>p</i> = 0.002, chi-squared test). AA incidence was correlated to elevated CD14<sup>+</sup> monocyte numbers (<i>R</i> = 0.092, <i>p</i> = 0.036) and markedly independently correlated with increased CRHR1 expression (<i>R</i> = 0.215, <i>p</i> = 0.027). High CRHR1 expression was significantly related to chronic AA (disease duration &gt;1 year; <i>p</i> = 0.003, chi-squared test), and large lesion area (AA area &gt;25%; <i>p</i> = 0.049, chi-squared test). We also observed enhanced percentages of active monocytes and reduced CD16<sup>+</sup> CD3− NK cell numbers in AA patients' PBMCs (<i>p</i> = 0.010; 0.025, respectively). In vitro CRH treatment of PBMCs and human monocyte cell line THP-1 promoted CD86 upregulation. The findings imply that stress-related factors CRH and CRHR1 contribute to AA development and progression where higher CRHR1 expression is associated with chronic AA and larger lesions.</p>","PeriodicalId":12243,"journal":{"name":"Experimental Dermatology","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/exd.15182","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental Dermatology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/exd.15182","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DERMATOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Stress may play a key role in alopecia areata (AA), though the exact interactions of stress with AA remain undefined. Corticotropin-releasing hormone (CRH), the proximal regulator of the stress axis, has been recognized as an immunomodulatory factor in tissues and peripheral blood mononuclear cells (PBMCs). We used multicolour flow cytometry to identify receptor CRHR1 expression on PBMC subsets in AA patients (n = 54) and controls (n = 66). We found that CRHR1 was primarily expressed by circulating monocytes. CRHR1 expression on monocytes was enhanced in AA compared with controls (3.17% vs. 1.44%, p = 0.002, chi-squared test). AA incidence was correlated to elevated CD14+ monocyte numbers (R = 0.092, p = 0.036) and markedly independently correlated with increased CRHR1 expression (R = 0.215, p = 0.027). High CRHR1 expression was significantly related to chronic AA (disease duration >1 year; p = 0.003, chi-squared test), and large lesion area (AA area >25%; p = 0.049, chi-squared test). We also observed enhanced percentages of active monocytes and reduced CD16+ CD3− NK cell numbers in AA patients' PBMCs (p = 0.010; 0.025, respectively). In vitro CRH treatment of PBMCs and human monocyte cell line THP-1 promoted CD86 upregulation. The findings imply that stress-related factors CRH and CRHR1 contribute to AA development and progression where higher CRHR1 expression is associated with chronic AA and larger lesions.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
AA 患者的单核细胞中 CRHR1 表达增加,使其对促肾上腺皮质激素释放激素产生促炎症反应。
压力可能在斑秃(AA)中起着关键作用,但压力与斑秃的确切相互作用仍未确定。促肾上腺皮质激素释放激素(CRH)是压力轴的近端调节因子,已被认为是组织和外周血单核细胞(PBMC)中的免疫调节因子。我们使用多色流式细胞术鉴定了 AA 患者(54 人)和对照组(66 人)PBMC 亚群中受体 CRHR1 的表达。我们发现 CRHR1 主要由循环单核细胞表达。与对照组相比,AA 患者单核细胞上的 CRHR1 表达增强(3.17% 对 1.44%,P = 0.002,卡方检验)。AA 发病率与 CD14+ 单核细胞数量升高相关(R = 0.092,p = 0.036),与 CRHR1 表达增加明显独立相关(R = 0.215,p = 0.027)。CRHR1 的高表达与慢性 AA(病程大于 1 年;p = 0.003,卡方检验)和大面积病变(AA 面积大于 25%;p = 0.049,卡方检验)明显相关。我们还观察到,AA 患者的 PBMCs 中活性单核细胞百分比增加,CD16+ CD3- NK 细胞数量减少(分别为 p = 0.010;0.025)。体外 CRH 处理 PBMCs 和人类单核细胞系 THP-1 可促进 CD86 上调。研究结果表明,应激相关因子CRH和CRHR1有助于AA的发生和发展,其中CRHR1的高表达与慢性AA和更大的病变有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Experimental Dermatology
Experimental Dermatology 医学-皮肤病学
CiteScore
6.70
自引率
5.60%
发文量
201
审稿时长
2 months
期刊介绍: Experimental Dermatology provides a vehicle for the rapid publication of innovative and definitive reports, letters to the editor and review articles covering all aspects of experimental dermatology. Preference is given to papers of immediate importance to other investigators, either by virtue of their new methodology, experimental data or new ideas. The essential criteria for publication are clarity, experimental soundness and novelty. Letters to the editor related to published reports may also be accepted, provided that they are short and scientifically relevant to the reports mentioned, in order to provide a continuing forum for discussion. Review articles represent a state-of-the-art overview and are invited by the editors.
期刊最新文献
Disabled 2 (Dab2) Regulates Tumour Progression in Skin Squamous Cell Carcinoma Neurofibromatosis Type 1 Patients With Epilepsy: A Comprehensive Analysis of Demographics, Comorbidities and Healthcare Outcomes Incontinence-Associated Dermatitis-Like Skin Changes Induced by the Application of Absorbent Pads Containing Bacteria and Artificial Urine in Rats NEDD4L Inhibits the Proliferation and Migration of Keloid Fibroblasts by Regulating YY1 Ubiquitination-Mediated Glycolytic Metabolic Reprogramming Issue Information
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1