Hohaiivirga grylli gen. nov., sp. nov., a New Member of the Family Methylobacteriaceae, Isolated from Cricket (Gryllus chinensis).

Abstract

A Gram-staining negative, non-motile, rod-shaped, oxidase negative and catalase positive strain WL0021^T was isolated from cricket (Gryllus chinensis) living in the campus of Hohai University. Strain WL0021^T was characterized utilizing a polyphasic taxonomy approach. The major fatty acids (> 5%) for strain WL0021^T were C_16:0 and summed feature 8, and the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phospholipid, two aminolipids, and an unidentified polar lipid. Ubiquinone-10 was detected as the predominant respiratory quinone. The results of 16S rRNA gene phylogenetic analyses revealed that strain WL0021^T had the highest sequence similarity of 95.3% to Microvirga flavescens c27j1^T and strain WL0021^T formed a distinct linage within the family Methylobacteriaceae in the phylogenetic trees. Whole genomic DNA G+C content was 48.3%. Combined with the results from this study, strain WL0021^T should represent a novel genus in the family Methylobacteriaceae, for which the name Hohaiivirga grylli gen. nov., sp. nov. (type strain WL0021^T=GDMCC 1.2420^T =JCM 34655^T=MCCC 1K05886^T) is proposed.