Development of the Pipette-Tip Micro-Solid-Phase Extraction for Extraction of Rutin From Moringa oleifera Lam. Using Activated Hollow Carbon Nanospheres as Sorbents.

IF 1.5 4区 化学 Q3 CHEMISTRY, ANALYTICAL International Journal of Analytical Chemistry Pub Date : 2024-09-27 eCollection Date: 2024-01-01 DOI:10.1155/2024/2681595
Dakalo Lorraine Ndou, Bonakele Patricia Mtolo, Adivhaho Khwathisi, Ashwell Rungano Ndhlala, Nikita Tawanda Tavengwa, Ntakadzeni Edwin Madala
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Abstract

Herein, a micro-solid-phase extraction (μSPE) method was developed using a pipette tip for rutin extraction, employing activated hollow carbon nanospheres (HCNSs) as the sorbent. Characterization of the activated carbon nanospheres through TGA, FTIR, and SEM analysis confirmed the success of the activation process. The study demonstrated the efficacy of PT-μSPE in rutin extraction under pH 2 conditions with a standard concentration of 2 mg·L-1. The optimal mass of HCNSs was found to be 2 mg, and a loading volume of 500 μL resulted in the maximum recovery of rutin. Propan-2-ol was the best elution solvent with 15 aspirating/dispensing cycles. The correlation of determination (R 2) for the calibration curve was found to be 0.9991, and the LOD and LOQ values were 0.604 and 1.830 mg·L-1, respectively. The applicability of the method was demonstrated by extracting rutin from a complex Moringa oleifera leaf extract with the relative standard deviation (RSD) of 3.26%, thereby validating this method as feasible for the extraction of useful bioactive compounds from complex plant samples.

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以活性空心碳纳米球为吸附剂从油辣木中提取芦丁的移液管-吸头微固相萃取法的开发使用活性空心碳纳米球作为吸附剂。
本文以活性空心碳纳米球(HCNSs)为吸附剂,开发了一种使用移液管吸头提取芦丁的微固相萃取(μSPE)方法。通过热重分析、傅立叶变换红外光谱和扫描电镜分析对活性炭纳米球进行表征,证实了活化过程的成功。研究证明了 PT-μSPE 在 pH 值为 2 的条件下(标准浓度为 2 mg-L-1)萃取芦丁的有效性。研究发现,HCNS 的最佳质量为 2 毫克,装载量为 500 μL 时芦丁的回收率最高。丙-2-醇是最佳的洗脱溶剂,可进行 15 次抽吸/分配循环。定量曲线的相关系数(R 2)为 0.9991,最低检出限(LOD)和最低定量限(LOQ)分别为 0.604 和 1.830 mg-L-1。从复杂的辣木叶提取物中提取芦丁的相对标准偏差(RSD)为 3.26%,证明了该方法的适用性,从而验证了该方法适用于从复杂植物样品中提取有用的生物活性化合物。
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来源期刊
CiteScore
3.10
自引率
5.60%
发文量
117
期刊介绍: International Journal of Analytical Chemistry publishes original research articles that report new experimental results and methods, especially in relation to important analytes, difficult matrices, and topical samples. Investigations may be fundamental, or else related to specific applications; examples being biological, environmental and food testing, and analysis in chemical synthesis and materials processing. As well as original research, the International Journal of Analytical Chemistry also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.
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