Pyriofenone Interacts with the Major Facilitator Superfamily Transporter of Phytopathogenic Fungi to Potentially Control Tea Leaf Spot Caused by Lasiodiplodia theobromae.

IF 2.6 2区 农林科学 Q2 PLANT SCIENCES Phytopathology Pub Date : 2024-10-07 DOI:10.1094/PHYTO-08-24-0246-R
Xiaolin Zhang, Fenghua Liu, Dongxue Li, Di Guo, Yue Ma, Jing-Jiang Zhou, Delu Wang, Zhuo Chen
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Abstract

Tea leaf spot caused by Lasiodiplodia theobromae is a newly discovered fungal disease in southwest China. Due to a lack of knowledge of its epidemiology and control strategies, the disease has a marked impact on tea yield and quality. Pyriofenone is a new fungicide belonging to the aryl phenyl ketone fungicide group, which has shown marked efficacy in controlling various fungal diseases. However, its mechanism of action is not yet understood. This study found that pyriofenone exhibits strong in vitro inhibitory activity against various phytopathogenic fungi. Specifically, it showed strong inhibitory activity against L. theobromae, with a half-maximal effective concentration (EC50) value of 0.428 μg/ml determined by measuring mycelial growth rate. Morphological observations, using optical, scanning electron, and transmission electron microscopy, revealed that pyriofenone induces morphological abnormalities in L. theobromae hyphae. At lower doses, the hyphae became swollen, the distance between septa decreased, and the hyphal growth rate slowed. At higher doses and longer exposures, the hyphae collapsed. Transcriptomic and bioinformatic analyses indicated that pyriofenone can affect the expression of genes related to membrane transporters. Homology modeling suggested that pyriofenone may bind to a candidate target protein of the major facilitator superfamily (MFS) transporter, with a free binding energy of -7.1 kcal/mol. This study suggests that pyriofenone may potentially regulate the transport of metabolites in L. theobromae, thus affecting hyphal metabolism and interfering with hyphal growth. Pyriofenone exhibits in vitro inhibitory activity against various tea foliar pathogens and holds promise for future applications to the control of tea foliar diseases.

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Pyriofenone 与植物病原真菌的主要促进剂超家族转运体相互作用,有可能控制由 Lasiodiplodia theobromae 引起的茶叶斑点病。
由Lasiodiplodia theobromae引起的茶叶叶斑病是中国西南地区新发现的一种真菌病害。由于对其流行病学和防治策略缺乏了解,该病害对茶叶的产量和质量造成了显著影响。Pyriofenone 是一种新型杀菌剂,属于芳基苯基酮类杀菌剂,对多种真菌病害有显著的防治效果。然而,人们对其作用机制尚不了解。本研究发现,吡蚜酮对多种植物病原真菌具有很强的体外抑制活性。具体来说,它对 L. theobromae 具有很强的抑制活性,通过测定菌丝生长速度,其半最大有效浓度(EC50)值为 0.428 μg/ml。使用光学、扫描电子和透射电子显微镜进行的形态学观察显示,吡蚜酮会诱导大叶菠萝褐藻菌丝出现形态异常。在较低剂量下,菌丝会肿胀,隔膜间距减小,菌丝生长速度减慢。在较高剂量和较长时间的暴露下,菌丝会塌陷。转录组学和生物信息学分析表明,吡蚜酮会影响与膜转运体有关的基因的表达。同源建模表明,吡蚜酮可能与主要促进剂超家族(MFS)转运体的候选靶蛋白结合,其自由结合能为-7.1 kcal/mol。这项研究表明,三苯甲酮可能会调节可可碱草菌代谢物的转运,从而影响菌体代谢并干扰菌体生长。Pyriofenone 对多种茶叶叶面病原体具有体外抑制活性,有望在未来应用于茶叶叶面病害的防治。
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来源期刊
Phytopathology
Phytopathology 生物-植物科学
CiteScore
5.90
自引率
9.40%
发文量
505
审稿时长
4-8 weeks
期刊介绍: Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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