Antioxidant and Longevity-Related Properties of the Ethyl Acetate Fraction of Cnidium officinale Makino in Caenorhabditis elegans.

IF 1.6 Q3 FOOD SCIENCE & TECHNOLOGY Preventive Nutrition and Food Science Pub Date : 2024-09-30 DOI:10.3746/pnf.2024.29.3.311
Hyeon-Ji Kim, Ji-Su Mun, Suk-Heung Oh, Jun-Hyeong Kim
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Abstract

Reactive oxygen species (ROS) are produced from energy metabolism and may cause diseases or cell death. Antioxidation refers to the suppression of ROS production and is considered beneficial in preventing diseases. This study aimed to examine the antioxidative effects of Cnidium officinale Makino (COM) extracts and fractions using Caenorhabditis elegans as an experimental model. The COM ethanol extract was fractionated according to polarity. The results showed that the ethyl acetate fraction of COM showed powerful radical scavenging activities and increased the activities of superoxide dismutase (SOD) and catalase in C. elegans in a concentration-dependent manner. Moreover, the ethyl acetate fraction reduced the ROS production rate in C. elegans and increased the cell survival rate, suggesting oxidative and thermal stress resistance. In addition, the SOD-3::green fluorescent protein (GFP) expression level in the transformed cells of C. elegans (CF1553) increased, suggesting oxidative stress resistance. Similarly, the HSP-16.2::GFP expression level increased, suggesting thermal stress resistance. In conclusion, the ethyl acetate fraction of COM demonstrated the strongest antioxidative effects, indicating that it may help extend longevity.

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Cnidium officinale Makino 的乙酸乙酯馏分在秀丽隐杆线虫体内的抗氧化和长寿特性
活性氧(ROS)产生于能量代谢,可能导致疾病或细胞死亡。抗氧化是指抑制 ROS 的产生,被认为有益于预防疾病。本研究旨在以秀丽隐杆线虫(Caenorhabditis elegans)为实验模型,研究Cnidium officinale Makino(COM)提取物和馏分的抗氧化作用。根据极性对 Cnidium officinale Makino(COM)乙醇提取物进行了分馏。结果表明,COM 的乙酸乙酯馏分具有强大的自由基清除活性,并以浓度依赖的方式提高了秀丽隐杆线虫体内超氧化物歧化酶(SOD)和过氧化氢酶的活性。此外,乙酸乙酯馏分降低了秀丽隐杆线虫体内 ROS 的产生率,提高了细胞存活率,这表明秀丽隐杆线虫具有抗氧化和抗热应激的能力。此外,SOD-3::绿色荧光蛋白(GFP)在秀丽隐杆线虫(CF1553)转化细胞中的表达水平增加,表明其具有抗氧化应激能力。同样,HSP-16.2::GFP 的表达水平也有所提高,表明其具有抗热应激能力。总之,COM 的乙酸乙酯馏分具有最强的抗氧化作用,表明它可能有助于延年益寿。
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来源期刊
Preventive Nutrition and Food Science
Preventive Nutrition and Food Science Agricultural and Biological Sciences-Food Science
CiteScore
3.40
自引率
0.00%
发文量
35
期刊最新文献
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