Effect of Siegesbeckia glabrescens Extract on Foam Cell Formation in THP-1 Macrophages.

IF 1.6 Q3 FOOD SCIENCE & TECHNOLOGY Preventive Nutrition and Food Science Pub Date : 2024-09-30 DOI:10.3746/pnf.2024.29.3.288
Ha-Rin Moon, Jung-Mi Yun
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Abstract

The accumulation of cholesterol-bearing macrophage foam cells in the initial stages of atherosclerosis serves as a characteristic feature of atherosclerotic lesions. The inhibitory effect of Siegesbeckia glabrescens, a species of flowering plant in the Asteraceae family, on foam cell formation in THP-1 macrophages has not yet been elucidated. In this study, we explored the effect of S. glabrescens ethanol extract (SGEE) and hot water extract (SGWE) on foam cell formation via co-treatment with oxidized low density lipoprotein (ox-LDL) and lipopolysaccharide (LPS), mimicking the occurrence of atherosclerosis in vitro, and studied the regulation of its underlying mechanisms. THP-1 cells differentiated by PMA (1 μM) for 48 h were subsequently treated with/without SGWE and SGEE for 48 h. THP-1 macrophages were treated with ox-LDL (20 μg/mL) and LPS (500 ng/mL) for 24 h. Treatment with ox-LDL and LPS for 24 h enhanced the lipid accumulation in foam cells compared to in untreated cells, as determined by oil red O staining. In contrast, SGWE and SGEE treatment inhibited lipid accumulation in foam cells. Both extracts significantly upregulated ABCA1, LXRα, and PPARγ expression in ox-LDL- and LPS-treated cells (P<0.05). Moreover, both SGWE and SGEE decreased LOX-1, CD36, and SR-A1 expression. The co-treatment of ox-LDL and LPS increased NF-κB, COX-2, and pro-inflammatory activation and expression compared with untreated cells. However, this increase suppressed NF-κB, COX-2, and pro-inflammatory expression by SGWE and SGEE. The results indicated that both extracts can partially inhibit foam cell formation and contribute to protective effects by suppressing cholesterol accumulation during the onset of atherosclerosis.

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豨莶草提取物对 THP-1 巨噬细胞泡沫细胞形成的影响
在动脉粥样硬化的初期阶段,含有胆固醇的巨噬细胞泡沫细胞的积累是动脉粥样硬化病变的一个特征。菊科开花植物豨莶草(Siegesbeckia glabrescens)对 THP-1 巨噬细胞泡沫细胞形成的抑制作用尚未阐明。本研究通过体外氧化低密度脂蛋白(ox-LDL)和脂多糖(LPS)共同处理,模拟动脉粥样硬化的发生,探讨了茜草乙醇提取物(SGEE)和热水提取物(SGWE)对泡沫细胞形成的影响,并研究了其潜在机制的调控。用氧化-LDL(20 μg/mL)和LPS(500 ng/mL)处理THP-1巨噬细胞24小时。与未处理的细胞相比,用氧化-LDL和LPS处理24小时会增加泡沫细胞中的脂质积累,这是由油红O染色测定的。相比之下,SGWE 和 SGEE 可抑制泡沫细胞中的脂质积累。这两种提取物都能明显上调氧化-LDL 和 LPS 处理细胞中 ABCA1、LXRα 和 PPARγ 的表达(P<0.05)。
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来源期刊
Preventive Nutrition and Food Science
Preventive Nutrition and Food Science Agricultural and Biological Sciences-Food Science
CiteScore
3.40
自引率
0.00%
发文量
35
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