Effects of Green Tea and Java Pepper Mixture on Gut Microbiome and Colonic MicroRNA-221/222 in Mice with Dextran Sulfate Sodium-Induced Colitis.

IF 1.6 Q3 FOOD SCIENCE & TECHNOLOGY Preventive Nutrition and Food Science Pub Date : 2024-09-30 DOI:10.3746/pnf.2024.29.3.279
Jumi Lee, Mak-Soon Lee, Yangha Kim
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Abstract

In this study, we aimed to investigate the regulatory effects of a green tea and java pepper mixture (GTP) on the gut microbiome and microRNA (miR)-221/222 expression in mice with dextran sulfate sodium (DSS)-induced colitis. Male C57BL/6J mice were divided into four groups: DSS-, DSS+, GTP50, and GTP100. In the GTP50 and GTP100 groups, GTP was orally administered to mice at doses of 50 and 100 mg/kg body weight, respectively, every day for 2 weeks, and colitis was induced in the DSS+, GTP50, and GTP100 groups by adding 3% DSS to their drinking water for 1 week. GTP was found to mitigate the severity of inflammation and the damage to goblet cells caused by DSS-induced colitis. The results showed that compared with the DSS- group, the relative abundance of Bacteroidetes was increased and that of Proteobacteria and Candidatus Melainabacteria was decreased in the GTP100 group. The ratio of Firmicutes to Bacteroidetes was also reduced in the GTP100 group. However, GTP administration did not modulate the microbial diversity. GTP administration upregulated the mRNA and protein levels of occludin and zonula occludens 1. In addition, GTP effectively downregulated the expression of miR-221 and miR-222. Overall, GTP altered the gut microbiota composition and downregulated colonic miR-221/222 expression in mice with DSS-induced colitis.

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绿茶和爪哇胡椒混合物对葡聚糖硫酸钠诱发结肠炎小鼠肠道微生物组和结肠 MicroRNA-221/222 的影响
本研究旨在探讨绿茶和爪哇胡椒混合物(GTP)对葡聚糖硫酸钠(DSS)诱导的结肠炎小鼠肠道微生物组和 microRNA(miR)-221/222 表达的调节作用。雄性 C57BL/6J 小鼠分为四组:DSS-组、DSS+组、GTP50组和GTP100组。在 GTP50 和 GTP100 组中,每天分别以 50 和 100 毫克/公斤体重的剂量给小鼠口服 GTP,持续 2 周;在 DSS+、GTP50 和 GTP100 组中,在饮用水中添加 3% 的 DSS,诱导小鼠结肠炎,持续 1 周。研究发现,GTP能减轻DSS诱导的结肠炎引起的炎症和对腺泡细胞的损伤。结果表明,与DSS组相比,GTP100组中类杆菌的相对丰度增加,而蛋白杆菌和拟杆菌的相对丰度降低。在 GTP100 组中,固着菌与类杆菌的比例也有所下降。然而,施用 GTP 并没有改变微生物的多样性。施用 GTP 可上调闭锁素和闭锁带 1 的 mRNA 和蛋白质水平。此外,GTP 还有效下调了 miR-221 和 miR-222 的表达。总之,GTP改变了DSS诱导的结肠炎小鼠的肠道微生物群组成,并下调了结肠miR-221/222的表达。
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来源期刊
Preventive Nutrition and Food Science
Preventive Nutrition and Food Science Agricultural and Biological Sciences-Food Science
CiteScore
3.40
自引率
0.00%
发文量
35
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