Ethanolic Extract from Echinacea purpurea (L.) Moench Inhibits Influenza A/B and Respiratory Syncytial Virus Infection in vitro: Preventive Agent for Viral Respiratory Infections.

IF 1.6 Q3 FOOD SCIENCE & TECHNOLOGY Preventive Nutrition and Food Science Pub Date : 2024-09-30 DOI:10.3746/pnf.2024.29.3.332
Sung-Kwon Lee, Dong-Ryung Lee, Da-Eun Min, Seong-Hyeon Park, Deok-Geun Kim, Eun-Ji Kim, Bong-Keun Choi, Kang-Beom Kwon
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Abstract

Among the most frequent causes of respiratory infections in humans are influenza A virus H1N1 (H1N1), influenza B virus (IVB), and respiratory syncytial virus (RSV). Echinacea is a perennial wildflower belonging to the Asteraceae family. Echinacea purpurea (L.) Moench is a species belonging to the Echinacea genus. Its characteristic compound, chicoric acid (CA), is known for its physiological activities, including antiviral effects and immune enhancement. Activities of E. purpurea 60% ethanol extract (EPE) and CA in inhibiting infections caused by H1N1, IVB, and RSV subtype A (RSV-A) were evaluated through plaque inhibition tests, quantification of viral gene expression, and analysis of transmission electron microscopy (TEM) images. Additionally, inhibitory activities of EPE and CA for hemagglutination and neuraminidase (NA) of H1N1 and IVB were determined. In the plaque reduction assays, both EPE and CA reduced infectivity against H1N1, IVB, and RSV-A. Furthermore, quantitative real-time polymerase chain reaction analysis revealed that EPE and CA reduced gene expression levels for H1N1, IVB, and RSV-A, whereas TEM image analysis confirmed their inhibitory effects on host cell infection by these viruses. Hemagglutination assays exhibited the ability of EPE and CA to hinder H1N1 and IVB attachment to host cell receptors. Furthermore, EPE and CA displayed inhibition activity against the NA of H1N1 and IVB. These findings suggest that EPE and CA can suppress the infection and propagation of H1N1, IVB, and RSV-A, demonstrating their potential as preventive and therapeutic agents for viral respiratory infections or as ingredients for health functional foods.

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紫锥菊(Echinacea purpurea (L.) Moench)乙醇提取物可在体外抑制甲型/乙型流感和呼吸道合胞病毒感染:病毒性呼吸道感染的预防剂。
人类呼吸道感染最常见的原因包括甲型 H1N1 流感病毒(H1N1)、乙型流感病毒(IVB)和呼吸道合胞病毒(RSV)。紫锥花属于菊科多年生野花。Echinacea purpurea (L.) Moench 属于紫锥花属。其特征化合物菊苣酸(CA)具有抗病毒和增强免疫力等生理活性。通过斑块抑制试验、病毒基因表达定量和透射电子显微镜(TEM)图像分析,评估了紫锥菊 60% 乙醇提取物(EPE)和 CA 在抑制 H1N1、IVB 和 RSV 亚型 A(RSV-A)感染方面的活性。此外,还测定了 EPE 和 CA 对 H1N1 和 IVB 血凝和神经氨酸酶(NA)的抑制活性。在斑块缩小试验中,EPE 和 CA 都能降低 H1N1、IVB 和 RSV-A 的感染率。此外,实时聚合酶链反应定量分析显示,EPE 和 CA 可降低 H1N1、IVB 和 RSV-A 的基因表达水平,而 TEM 图像分析则证实了它们对宿主细胞感染这些病毒的抑制作用。血凝试验表明,EPE 和 CA 能够阻止 H1N1 和 IVB 吸附到宿主细胞受体上。此外,EPE 和 CA 还对 H1N1 和 IVB 的 NA 具有抑制活性。这些研究结果表明,EPE 和 CA 可抑制 H1N1、IVB 和 RSV-A 的感染和传播,显示了它们作为病毒性呼吸道感染的预防和治疗剂或保健功能食品成分的潜力。
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来源期刊
Preventive Nutrition and Food Science
Preventive Nutrition and Food Science Agricultural and Biological Sciences-Food Science
CiteScore
3.40
自引率
0.00%
发文量
35
期刊最新文献
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