Crystal structure and function of a phosphate starvation responsive protein phosphatase, GmHAD1-2 regulating soybean root development and flavonoid metabolism

IF 8.3 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2024-10-06 DOI:10.1111/nph.20174

Zeyu Zhang, Xiaohui Mo, Hongbo Zhao, Xing Lu, Shilong Fan, Xiaojia Huang, Huafu Mai, Hong Liao, Yinghe Zhang, Cuiyue Liang, Jiang Tian

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Abstract

Phosphate (Pi) availability is well known to regulate plant root growth. However, it remains largely unknown how flavonoid synthesis participates in affecting plant root growth in response to Pi starvation.
In the study, the crystal structure of a plant protein phosphatase, GmHAD1-2, was dissected using X-ray crystallography for the first time. It was revealed that GmHAD1-2 contained a modified Rossmannoid class of α/β folds with three layered α/β sandwich.
Transcripts of GmHAD1-2 were increased by Pi starvation in soybean roots, especially in lateral root tips. GmHAD1-2 suppression or overexpression significantly influenced soybean lateral root length and number, as well as phosphorus (P) content. Furthermore, GmHAD1-2 was found to interact with a chalcone reductase, GmCHR1. Suppression of GmHAD1-2 significantly changed the flavonoid biosynthesis pathway in soybean roots.
Taken together, the results highlight that GmHAD1-2 can regulate soybean root growth by influencing flavonoid metabolism.

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调控大豆根系发育和类黄酮代谢的磷酸盐饥饿响应蛋白磷酸酶 GmHAD1-2 的晶体结构和功能。

众所周知，磷酸盐（Pi）的供应可调节植物根系的生长。然而，黄酮类化合物的合成如何参与影响植物根系的生长以应对 Pi 饥饿，这在很大程度上仍是个未知数。在这项研究中，我们首次利用 X 射线晶体学技术剖析了植物蛋白磷酸酶 GmHAD1-2 的晶体结构。研究发现，GmHAD1-2 含有经过修饰的 Rossmannoid 类 α/β 折叠层，其中有三层 α/β 夹层。大豆根部，尤其是侧根尖端的 GmHAD1-2 转录物在 Pi 饥饿下增加。抑制或过表达 GmHAD1-2 会显著影响大豆侧根的长度和数量以及磷（P）含量。此外，还发现 GmHAD1-2 与一种查尔酮还原酶 GmCHR1 相互作用。抑制 GmHAD1-2 能显著改变大豆根中黄酮类化合物的生物合成途径。综上所述，研究结果表明，GmHAD1-2 可通过影响黄酮类化合物的代谢来调控大豆根系的生长。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.