A new consecutive high-performance counter-current chromatography approach based on co-current and overlap mode for isolating active compounds from olive leaves

IF 5.4 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Biomaterials Science & Engineering Pub Date : 2024-10-09 DOI:10.1016/j.foodchem.2024.141581

Xiao Sun, Xin-Yi Huang, Jian-Fei Liu, Dong Pei, Duo-Long Di

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Abstract

Conventional consecutive separation methods based on overlapping injection modes produce interactions between samples as the separation time increases. This will lead to a reduction in the purity of the target due to the gradual saturation of the stationary phase distribution. To enhance the purity of the sample preparation and separation efficiency, we devised a consecutive separation method under high-performance counter-current chromatography (HPCCC) utilizing co-current elution and overlapping injection. The method was used for the separation and preparation of olive leaves for their main bioactive polyphenolic constituents. Six HPCCC separations were conducted in reversed-phase mode using a two-phase solvent system consisting of petroleum ether/ethyl acetate/water (1:40:40, v/v). Finally, 302.7 mg of luteolin-4’-O-β-D-glucoside and oleuropein were isolated from 720 mg of crude sample with purities of 93.3 % and 96.2 %, respectively. The optimization strategy outlined in this study can be used to enhance the extraction of bioactive components from olive leaves.

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基于共流和重叠模式的新型连续高效逆流色谱法，用于从橄榄叶中分离活性化合物

传统的连续分离方法基于重叠进样模式，随着分离时间的延长，样品之间会产生相互作用。这将导致固定相分布逐渐饱和，从而降低目标物的纯度。为了提高样品制备的纯度和分离效率，我们设计了一种在高效逆流色谱（HPCCC）下利用同流洗脱和重叠进样的连续分离方法。该方法用于分离和制备橄榄叶中的主要生物活性多酚成分。采用石油醚/乙酸乙酯/水（1:40:40，v/v）两相溶剂系统，在反相模式下进行了六次 HPCCC 分离。最后，从 720 毫克粗样品中分离出 302.7 毫克叶黄素-4'-O-β-D-葡萄糖苷和油菜素，纯度分别为 93.3 % 和 96.2 %。本研究中概述的优化策略可用于提高橄榄叶中生物活性成分的提取率。

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来源期刊

ACS Biomaterials Science & Engineering Materials Science-Biomaterials

CiteScore

10.30

自引率

3.40%

发文量

413

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