Use of Cyrene™, as an alternative to dimethyl sulfoxide, as a diluent for Melatonin to determine its in vitro antimicrobial capacity

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-10-09 DOI:10.1007/s00203-024-04151-9

Ana Muñoz-Jurado, Francisco Jurado-Martos, Eduardo Agüera, Isaac Túnez, Begoña M. Escribano

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Abstract

Melatonin (MLT) is a methoxyindole that has potent antioxidant actions, anti-inflammatory, and antiapoptotic capacity. However, its in vitro antibacterial capacity has been the least studied of its properties. Dimethylsulfoxide (DMSO) has been the most used solvent for these tests, but it shows an antimicrobial effect if it is not dissolved. Cyrene™ is a new solvent that has emerged as an alternative to DMSO. Therefore, this study aimed to determine the antimicrobial capacity of MLT by MIC assays, using Cyrene™ as a solvent. Likewise, the solubility of MLT in this solvent and whether it exerted any effect on bacterial growth at different percentages was also determined. Different dilutions of MLT in Cyrene™ with different concentrations, were prepared. No growth inhibition caused by MLT was observed. The growth inhibition observed was because of Cyrene™. The maximum amount of MLT that can be diluted in 100% Cyrene is 10 mg/mL, but this percentage of solvent shows a bactericidal effect. Therefore, it must be dissolved at 5% to avoid this effect, so only 4 mg/mL of MLT can be diluted in it. Therefore, if no other solvents are available, the in vitro antibacterial role of MLT cannot be adequately assessed.

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使用 Cyrene™ 代替二甲亚砜作为褪黑素的稀释剂，以确定其体外抗菌能力。

褪黑素（MLT）是一种甲氧基吲哚，具有强大的抗氧化、抗炎和抗细胞凋亡能力。然而，对其体外抗菌能力的研究却最少。二甲基亚砜（DMSO）一直是这些测试中最常用的溶剂，但如果不溶解，也会显示出抗菌效果。Cyrene™ 是一种新出现的溶剂，可替代二甲基亚砜。因此，本研究旨在以 Cyrene™ 为溶剂，通过 MIC 试验确定 MLT 的抗菌能力。同样，本研究还测定了 MLT 在该溶剂中的溶解度，以及不同比例的 MLT 对细菌生长是否有影响。在 Cyrene™ 中制备了不同浓度的 MLT 稀释液。没有观察到 MLT 对生长有抑制作用。观察到的生长抑制是由 Cyrene™ 引起的。在 100%的芘中稀释 MLT 的最大量为 10 毫克/毫升，但这一百分比的溶剂具有杀菌作用。因此，必须以 5%的比例溶解才能避免这种效果，所以只能稀释 4 毫克/毫升的 MLT。因此，如果没有其他溶剂，就无法充分评估 MLT 的体外抗菌作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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