The Development and Application of Lyophilized LAMP Detection Reagent for Listeria monocytogenes.

IF 2.6 3区生物学 Q3 MICROBIOLOGY Current Microbiology Pub Date : 2024-10-09 DOI:10.1007/s00284-024-03925-0

Yuhong Guan, Yanqi Wu, Peilin Huang, Xinyu Zhang, Yuting Chen, Hui Chen, Nongyue He

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Abstract

Listeria monocytogenes, a zoonotic foodborne pathogen, presents a significant threat to global public health. Therefore, rapid and sensitive detection methods are crucial in mitigating the spread of L. monocytogenes induced diseases. This study introduced a loop-mediated isothermal amplification (LAMP) lyophilized powder detection reagent specifically designed for identifying Listeria monocytogenes. The reagent is user-friendly, quick, and can be easily transported and stored at room temperature. It exhibits no cross-reactivity with eight other types of bacteria and boasts a sensitivity of 10¹ CFU/mL. In a comparative study of 30 samples, the LAMP lyophilized powder detection reagent demonstrated higher sensitivity than the commercial Listeria monocytogenes qPCR detection kit. Additionally, the experimental time was reduced by approximately 30 min, making it highly suitable for rapid diagnosis. Preparation of lyophilized LAMP reagents may facilitate large-scale deployment, particularly in endemic areas or regions facing rapid outbreaks. This could greatly aid in controlling the transmission of pathogens, especially those transmitted through food.

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单核细胞增多性李斯特菌冻干 LAMP 检测试剂的开发与应用。

单核细胞增生李斯特菌是一种人畜共患的食源性病原体，对全球公共卫生构成重大威胁。因此，快速灵敏的检测方法对于减少单增李斯特菌引起的疾病传播至关重要。本研究引入了一种环路介导等温扩增（LAMP）冻干粉检测试剂，专门用于鉴定单增李斯特菌。该试剂使用方便、快捷，可在室温下轻松运输和储存。它与其他八种细菌没有交叉反应，灵敏度高达 101 CFU/mL。在对 30 份样品进行的比较研究中，LAMP 冻干粉检测试剂的灵敏度高于商用李斯特菌 qPCR 检测试剂盒。此外，实验时间缩短了约 30 分钟，非常适合快速诊断。冻干 LAMP 试剂的制备有助于大规模应用，特别是在地方病流行地区或面临快速爆发的地区。这将大大有助于控制病原体的传播，尤其是那些通过食物传播的病原体。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.