The Development and Application of Lyophilized LAMP Detection Reagent for Listeria monocytogenes.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-10-09 DOI:10.1007/s00284-024-03925-0

Yuhong Guan, Yanqi Wu, Peilin Huang, Xinyu Zhang, Yuting Chen, Hui Chen, Nongyue He

{"title":"The Development and Application of Lyophilized LAMP Detection Reagent for Listeria monocytogenes.","authors":"Yuhong Guan, Yanqi Wu, Peilin Huang, Xinyu Zhang, Yuting Chen, Hui Chen, Nongyue He","doi":"10.1007/s00284-024-03925-0","DOIUrl":null,"url":null,"abstract":"Listeria monocytogenes, a zoonotic foodborne pathogen, presents a significant threat to global public health. Therefore, rapid and sensitive detection methods are crucial in mitigating the spread of L. monocytogenes induced diseases. This study introduced a loop-mediated isothermal amplification (LAMP) lyophilized powder detection reagent specifically designed for identifying Listeria monocytogenes. The reagent is user-friendly, quick, and can be easily transported and stored at room temperature. It exhibits no cross-reactivity with eight other types of bacteria and boasts a sensitivity of 101 CFU/mL. In a comparative study of 30 samples, the LAMP lyophilized powder detection reagent demonstrated higher sensitivity than the commercial Listeria monocytogenes qPCR detection kit. Additionally, the experimental time was reduced by approximately 30 min, making it highly suitable for rapid diagnosis. Preparation of lyophilized LAMP reagents may facilitate large-scale deployment, particularly in endemic areas or regions facing rapid outbreaks. This could greatly aid in controlling the transmission of pathogens, especially those transmitted through food.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-024-03925-0","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}

引用次数: 0

Abstract

Listeria monocytogenes, a zoonotic foodborne pathogen, presents a significant threat to global public health. Therefore, rapid and sensitive detection methods are crucial in mitigating the spread of L. monocytogenes induced diseases. This study introduced a loop-mediated isothermal amplification (LAMP) lyophilized powder detection reagent specifically designed for identifying Listeria monocytogenes. The reagent is user-friendly, quick, and can be easily transported and stored at room temperature. It exhibits no cross-reactivity with eight other types of bacteria and boasts a sensitivity of 10¹ CFU/mL. In a comparative study of 30 samples, the LAMP lyophilized powder detection reagent demonstrated higher sensitivity than the commercial Listeria monocytogenes qPCR detection kit. Additionally, the experimental time was reduced by approximately 30 min, making it highly suitable for rapid diagnosis. Preparation of lyophilized LAMP reagents may facilitate large-scale deployment, particularly in endemic areas or regions facing rapid outbreaks. This could greatly aid in controlling the transmission of pathogens, especially those transmitted through food.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

单核细胞增多性李斯特菌冻干 LAMP 检测试剂的开发与应用。

单核细胞增生李斯特菌是一种人畜共患的食源性病原体，对全球公共卫生构成重大威胁。因此，快速灵敏的检测方法对于减少单增李斯特菌引起的疾病传播至关重要。本研究引入了一种环路介导等温扩增（LAMP）冻干粉检测试剂，专门用于鉴定单增李斯特菌。该试剂使用方便、快捷，可在室温下轻松运输和储存。它与其他八种细菌没有交叉反应，灵敏度高达 101 CFU/mL。在对 30 份样品进行的比较研究中，LAMP 冻干粉检测试剂的灵敏度高于商用李斯特菌 qPCR 检测试剂盒。此外，实验时间缩短了约 30 分钟，非常适合快速诊断。冻干 LAMP 试剂的制备有助于大规模应用，特别是在地方病流行地区或面临快速爆发的地区。这将大大有助于控制病原体的传播，尤其是那些通过食物传播的病原体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊