Detoxification of ars genotypes by arsenite-oxidizing bacteria through arsenic biotransformation.

IF 3.2 3区环境科学与生态学 Q3 ENGINEERING, ENVIRONMENTAL Environmental Geochemistry and Health Pub Date : 2024-10-09 DOI:10.1007/s10653-024-02251-5

Jin-Soo Chang, Hyun-Jung Kim, Ji-Hoon Lee

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Abstract

The detoxification process of transforming arsenite (As(III)) to arsenate (As(V)) through bacterial oxidation presents a potent approach for bioremediation of arsenic-polluted soils in abandoned mines. In this study, twelve indigenous arsenic-oxidizing bacteria (AOB) were isolated from arsenic-contaminated soils. Among these, Paenibacillus xylanexedens EBC-SK As2 (MF928871) and Ochrobactrum anthropi EBC-SK As11 (MF928880) were identified as the most effective arsenic-oxidizing isolates. Evaluations for bacterial arsenic resistance demonstrated that P. xylanexedens EBC-SK As2 (MF928871) could resist As(III) up to 40 mM, while O. anthropi EBC-SK As11 (MF928880) could resist As(III) up to 25 mM. From these bacterial strains, genotypes of arsenic resistance system (ars) were detected, encompassing ars leader genes (arsR and arsD), membrane genes (arsB and arsJ), and aox genes known to be crucial for arsenic detoxification. These ars genotypes in the isolated AOBs might play an instrumental role in arsenic-contaminated soils with potential to reduce arsenic contamination.

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亚砷酸盐氧化细菌通过砷的生物转化对砷基因型进行解毒。

通过细菌氧化作用将亚砷酸盐（As(III)）转化为砷酸盐（As(V)）的解毒过程是对废弃矿井中受砷污染的土壤进行生物修复的有效方法。本研究从砷污染土壤中分离出 12 种本地砷氧化细菌（AOB）。其中，Paenibacillus xylanexedens EBC-SK As2 (MF928871) 和 Ochrobactrum anthropi EBC-SK As11 (MF928880) 被鉴定为最有效的砷氧化分离菌。细菌抗砷性评估表明，P. xylanexedens EBC-SK As2 (MF928871) 可抗高达 40 mM 的 As(III)，而 O. anthropi EBC-SK As11 (MF928880) 可抗高达 25 mM 的 As(III)。从这些细菌菌株中检测到了砷抗性系统（ars）的基因型，包括 ars 领导基因（arsR 和 arsD）、膜基因（arsB 和 arsJ）以及已知对砷解毒至关重要的 aox 基因。分离出的 AOB 中的这些 ars 基因型可能在砷污染土壤中发挥重要作用，具有减少砷污染的潜力。

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来源期刊

Environmental Geochemistry and Health 环境科学-工程：环境

CiteScore

8.00

自引率

4.80%

发文量

279

审稿时长

4.2 months

期刊介绍： Environmental Geochemistry and Health publishes original research papers and review papers across the broad field of environmental geochemistry. Environmental geochemistry and health establishes and explains links between the natural or disturbed chemical composition of the earth’s surface and the health of plants, animals and people. Beneficial elements regulate or promote enzymatic and hormonal activity whereas other elements may be toxic. Bedrock geochemistry controls the composition of soil and hence that of water and vegetation. Environmental issues, such as pollution, arising from the extraction and use of mineral resources, are discussed. The effects of contaminants introduced into the earth’s geochemical systems are examined. Geochemical surveys of soil, water and plants show how major and trace elements are distributed geographically. Associated epidemiological studies reveal the possibility of causal links between the natural or disturbed geochemical environment and disease. Experimental research illuminates the nature or consequences of natural or disturbed geochemical processes. The journal particularly welcomes novel research linking environmental geochemistry and health issues on such topics as: heavy metals (including mercury), persistent organic pollutants (POPs), and mixed chemicals emitted through human activities, such as uncontrolled recycling of electronic-waste; waste recycling; surface-atmospheric interaction processes (natural and anthropogenic emissions, vertical transport, deposition, and physical-chemical interaction) of gases and aerosols; phytoremediation/restoration of contaminated sites; food contamination and safety; environmental effects of medicines; effects and toxicity of mixed pollutants; speciation of heavy metals/metalloids; effects of mining; disturbed geochemistry from human behavior, natural or man-made hazards; particle and nanoparticle toxicology; risk and the vulnerability of populations, etc.