Synthesis and Characterization of Iodinated Chitosan Nanoparticles and Their Effects on Cancer Cells.

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS International Journal of Biomaterials Pub Date : 2024-09-02 eCollection Date: 2024-01-01 DOI:10.1155/2024/3850286
Germán Alvarado Tenorio, Roberto Espinosa Neira, Carlos Alberto Ávila Orta, Gabriela Yolotzín Romero Zúñiga, Hortensia Ortega Ortiz
{"title":"Synthesis and Characterization of Iodinated Chitosan Nanoparticles and Their Effects on Cancer Cells.","authors":"Germán Alvarado Tenorio, Roberto Espinosa Neira, Carlos Alberto Ávila Orta, Gabriela Yolotzín Romero Zúñiga, Hortensia Ortega Ortiz","doi":"10.1155/2024/3850286","DOIUrl":null,"url":null,"abstract":"<p><p>The high degree of chemical modification of the chitosan chains due to protonated amine groups allows them to react with many negatively charged surfaces as anionic polymers and cell membranes, resulting in an attractive material for medical and pharmaceutics applications. Incorporating ionic iodine (I<sup>-</sup> and IO<sub>3</sub> <sup>-</sup>) on chitosan chains is a direct way to successfully obtain chitosan-iodine nanoparticles (CSNPs-I and CSNPs-IO<sub>3</sub>) through ionic gelation. The nanoparticles (NPs) present a hemispherical morphology with sizes around 30-70 nm for CSNPs-I and CSNPs-IO<sub>3</sub>, similar to chitosan NPs, in accordance with SEM and DLS techniques. The XRD characterization did not show noticeable differences in the crystallinity index (CI) for CSNPs and CSNPs-I, 48.4 and 49.3%, respectively, but for CSNPs-IO<sub>3</sub>, the CI decreased to 43.85%. The cytotoxic effects on human tumor cells of chitosan and iodine-modified chitosan nanoparticles (CSNPs-I and CSNPs-IO<sub>3</sub>) were evaluated for 24 h in a range from 0.15 mg/mL to 0.95 mg/mL concentrations, where CSNPs-IO<sub>3</sub> presented the lower viability for lung cancer A549, followed by cervical cancer HeLa cell and finally breast cancer MDA-MB-231, with a weight content of iodate ion in a range of 8.7 to 15 <i>μ</i>g. This work presents the possibility of exploring chitosan-iodine NPs in medical applications.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2024 ","pages":"3850286"},"PeriodicalIF":3.0000,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11458297/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biomaterials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2024/3850286","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0

Abstract

The high degree of chemical modification of the chitosan chains due to protonated amine groups allows them to react with many negatively charged surfaces as anionic polymers and cell membranes, resulting in an attractive material for medical and pharmaceutics applications. Incorporating ionic iodine (I- and IO3 -) on chitosan chains is a direct way to successfully obtain chitosan-iodine nanoparticles (CSNPs-I and CSNPs-IO3) through ionic gelation. The nanoparticles (NPs) present a hemispherical morphology with sizes around 30-70 nm for CSNPs-I and CSNPs-IO3, similar to chitosan NPs, in accordance with SEM and DLS techniques. The XRD characterization did not show noticeable differences in the crystallinity index (CI) for CSNPs and CSNPs-I, 48.4 and 49.3%, respectively, but for CSNPs-IO3, the CI decreased to 43.85%. The cytotoxic effects on human tumor cells of chitosan and iodine-modified chitosan nanoparticles (CSNPs-I and CSNPs-IO3) were evaluated for 24 h in a range from 0.15 mg/mL to 0.95 mg/mL concentrations, where CSNPs-IO3 presented the lower viability for lung cancer A549, followed by cervical cancer HeLa cell and finally breast cancer MDA-MB-231, with a weight content of iodate ion in a range of 8.7 to 15 μg. This work presents the possibility of exploring chitosan-iodine NPs in medical applications.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
碘化壳聚糖纳米粒子的合成与特性及其对癌细胞的影响
由于质子化胺基对壳聚糖链进行了高度化学修饰,使其能够作为阴离子聚合物和细胞膜与许多带负电荷的表面发生反应,从而成为一种在医疗和制药应用中极具吸引力的材料。在壳聚糖链上加入离子碘(I- 和 IO3-)是通过离子凝胶化成功获得壳聚糖-碘纳米粒子(CSNPs-I 和 CSNPs-IO3)的直接方法。根据 SEM 和 DLS 技术,CSNPs-I 和 CSNPs-IO3 纳米粒子(NPs)呈现半球形形态,大小约为 30-70 nm,与壳聚糖 NPs 相似。XRD 表征显示,CSNPs 和 CSNPs-I 的结晶度指数(CI)并无明显差异,分别为 48.4% 和 49.3%,但 CSNPs-IO3 的结晶度指数降至 43.85%。评估了壳聚糖和碘改性壳聚糖纳米粒子(CSNPs-I 和 CSNPs-IO3)对人类肿瘤细胞的细胞毒性作用,24 小时内,CSNPs-I 和 CSNPs-IO3 的浓度范围从 0.15 mg/mL 到 0.其中 CSNPs-IO3 对肺癌 A549 的存活率较低,其次是宫颈癌 HeLa 细胞,最后是乳腺癌 MDA-MB-231。这项研究为壳聚糖-碘 NPs 在医疗领域的应用提供了可能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
期刊最新文献
Synthesis of Calcium Phosphate by Microwave Hydrothermal Method: Physicochemical and Morphological Characterization. Isolation of B Cells Using Silane-Coated Magnetic Nanoparticles. Evaluation of Microleakage of Orthograde Root-Filling Materials in Immature Permanent Teeth: An In Vitro Study. Production of Composite Briquette Fuel from Brewery Wastewater Sludge and Spent Grains. Unveiling SmearOFF Efficacy in Smear Layer Removal through Ultrasonic Activation Examined by Scanning Electron Microscopy.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1