Efficacy of adipose-derived stem cells in preventing peripheral nerve adhesion and promoting nerve regeneration: A laboratory investigation in a rat model.

IF 1.5 4区 医学 Q3 ORTHOPEDICS Journal of Orthopaedic Science Pub Date : 2024-10-07 DOI:10.1016/j.jos.2024.09.005
Yuta Nakamura, Kaoru Tada, Mika Akahane, Tsuyoshi Hattori, Masashi Matsuta, Atsuro Murai, Soichiro Honda, Osamu Hori, Satoru Demura
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Abstract

Background: Neurolysis alone or administration of anti-adhesion products after neurolysis is performed to treat peripheral nerve adhesion; however, the recovery of nerve function is poor. This study aimed to investigate the efficacy of adipose-derived stem cells (ADSCs) for peripheral nerve adhesion in a rat model.

Methods: As a nerve adhesion procedure, the neural bed was coagulated, and the epineurium of the sciatic nerve was sutured to the coagulated neural bed using nylon. Neurolysis was performed 6 weeks after the nerve adhesion procedure, and saline (control group) or ADSCs (ADSC group) were administered around the nerve where neurolysis was performed. Evaluations were performed 6 weeks after the administration.

Results: The wet weight ratio of the tibialis anterior muscle and nerve conduction velocity, which are indicators of nerve regeneration, were significantly better, while tensile strength, which is an indicator of the severity of nerve adhesion, was significantly lower in the ADSC group than in the control group. In the nerve, the expression of interleukin-10 and transforming growth factor-β in the nerve was significantly higher and that of tumor necrosis factor-α was significantly lower in the ADSC group than in the control group. Furthermore, significantly fewer M1 macrophages and significantly more M2 macrophages were observed in the ADSC group than in the control group. In the perineural scar, significantly fewer perineural collagen fibers and significantly more vascularization were observed in the ADSC group than in the control group.

Conclusions: ADSCs prevented peripheral nerve adhesion by reducing perineural scarring and enhancing vascularization. Additionally, ADSCs promoted nerve regeneration by decreasing inflammatory cytokine levels and increasing anti-inflammatory cytokine levels, as ADSCs regulated macrophage polarization from M1 to M2 macrophages. These findings hold promise for using ADSCs to treat nerve adhesion.

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脂肪源性干细胞在防止周围神经粘连和促进神经再生方面的功效:大鼠模型实验室研究。
背景:单纯神经溶解术或在神经溶解术后服用抗粘连产品可治疗周围神经粘连,但神经功能恢复不佳。本研究旨在探讨脂肪源性干细胞(ADSCs)对大鼠模型周围神经粘连的疗效:方法:作为神经粘连手术,凝固神经床,用尼龙将坐骨神经的外膜缝合到凝固的神经床上。神经粘连术后 6 周进行神经切除,在进行神经切除的神经周围注射生理盐水(对照组)或 ADSCs(ADSC 组)。结果:结果:作为神经再生指标的胫骨前肌湿重比和神经传导速度在 ADSC 组明显好转,而作为神经粘连严重程度指标的抗张强度在 ADSC 组明显低于对照组。在神经中,ADSC组白细胞介素-10和转化生长因子-β的表达明显高于对照组,而肿瘤坏死因子-α的表达则明显低于对照组。此外,ADSC 组的 M1 巨噬细胞明显少于对照组,而 M2 巨噬细胞明显多于对照组。在神经周围瘢痕中,ADSC组的神经周围胶原纤维明显少于对照组,血管明显多于对照组:结论:ADSCs 可通过减少神经周围瘢痕和增强血管生成来防止周围神经粘连。此外,ADSCs 还能降低炎性细胞因子水平,提高抗炎细胞因子水平,从而促进神经再生,因为 ADSCs 能调节巨噬细胞从 M1 到 M2 的极化。这些发现为利用 ADSCs 治疗神经粘连带来了希望。
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来源期刊
Journal of Orthopaedic Science
Journal of Orthopaedic Science 医学-整形外科
CiteScore
3.00
自引率
0.00%
发文量
290
审稿时长
90 days
期刊介绍: The Journal of Orthopaedic Science is the official peer-reviewed journal of the Japanese Orthopaedic Association. The journal publishes the latest researches and topical debates in all fields of clinical and experimental orthopaedics, including musculoskeletal medicine, sports medicine, locomotive syndrome, trauma, paediatrics, oncology and biomaterials, as well as basic researches.
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