Evidence of compensation for mitochondrial reactive oxygen species increase in Caenorhabditis briggsae cytoplasmic-nuclear hybrids.

Abstract

Hybrid offspring dysfunction in cytoplasmic-nuclear hybrids (cybrids) implies that one parent's mitochondrial genome is incompatible with the nuclear genome of the other parent. In Caenorhabditis briggsae , cybrids exhibit increased mitochondrial reactive oxygen species (ROS). In this study, we measured the specific activity of markers for mitochondrial abundance (citrate synthase) and antioxidant enzyme response (catalase) in four C. briggsae cybrid lines. An increase of catalase expression but not in mitochondrial abundance was found in dysfunctional cybrids. This suggests that organisms might compensate for some genetic incompatibilities by modulating gene expression of key oxidative stress enzymes such as catalase.