Defining genome access of transcription factors

IF 31.7 1区 生物学 Q1 GENETICS & HEREDITY Nature genetics Pub Date : 2024-10-10 DOI:10.1038/s41588-024-01953-x
Petra Gross
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定义转录因子的基因组访问
转录因子(TFs)对其靶序列的访问受核小体占据率的制约,但局部染色质状态对单个 TFs 与靶结合能力的影响仍不清楚。在这项研究中,Dirk Schübeler 及其同事采用还原论方法,利用单分子足迹法确定了不同染色质环境中 100 多个单个 TF 结合基序的体内占据率。他们发现,包括先驱因子 OCT4 和 SOX2 在内的一组 TF 能够在核小体定位无相位的序列中与它们的靶标结合,但当这些因子的内源位点被核小体占据时,它们就无法结合。与此相反,其他 TF(如 BANP、REST 或 CTCF)可以置换核小体,因此无论核小体定位如何,它们都能结合。有趣的是,获得 TF 基底的一个重要因素似乎是更长的图案长度,而不是 DNA 结合域的类型。总之,这些发现挑战了将TF分为先驱因子和非先驱因子的观点,同时也强调了染色质环境对塑造基因组中特定TF结合模式的重要性:Mol. Cell 84, 3455-3468细胞 84, 3455-3468.e6 (2024)
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来源期刊
Nature genetics
Nature genetics 生物-遗传学
CiteScore
43.00
自引率
2.60%
发文量
241
审稿时长
3 months
期刊介绍: Nature Genetics publishes the very highest quality research in genetics. It encompasses genetic and functional genomic studies on human and plant traits and on other model organisms. Current emphasis is on the genetic basis for common and complex diseases and on the functional mechanism, architecture and evolution of gene networks, studied by experimental perturbation. Integrative genetic topics comprise, but are not limited to: -Genes in the pathology of human disease -Molecular analysis of simple and complex genetic traits -Cancer genetics -Agricultural genomics -Developmental genetics -Regulatory variation in gene expression -Strategies and technologies for extracting function from genomic data -Pharmacological genomics -Genome evolution
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