The role of foraging pollinators in assembling the flower microbiota and transmitting the fire blight pathogen Erwinia amylovora

IF 4.3 2区 生物学 Q2 MICROBIOLOGY Environmental microbiology Pub Date : 2024-10-10 DOI:10.1111/1462-2920.16702
Salma Mukhtar, M. Amine Hassani, Tracy Zarrillo, Zhouqi Cui, George W. Sundin, Quan Zeng
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Abstract

Flowers serve as hubs for biotic interactions with pollinators and microbes, which can significantly impact plant reproduction and health. Previous studies have shown that the flower microbiota undergoes dynamic assembly processes during anthesis. However, the influence of foraging pollinators on the assembly and dispersal of the flower microbiota and the transmission of plant pathogens remains poorly understood. In this study, we used insect exclusion netting to investigate the role of pollinators in the assembly of the microbiota on apple stigma and the transmission of the fire blight pathogen Erwinia amylovora. We found that excluding pollinators had a minor impact on the community diversity and composition of the apple stigma microbiota, while the flower's developmental stage had a strong influence. Additionally, pollinator exclusion altered bacterial dispersal and the relative abundance of different bacterial species, including E. amylovora, suggesting that pollinators play a role in transmitting plant pathogens. Using a reporter system, we demonstrated that bumble bees can transmit the fire blight pathogen from an infected flower under controlled growth conditions. Our study highlights the importance of intrinsic and pollinator-independent microbes as sources of inoculum for the stigma microbiota and underscores the role of foraging pollinators in vectoring plant pathogens.

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觅食传粉昆虫在组合花卉微生物群和传播火疫病病原体 Erwinia amylovora 中的作用。
花朵是传粉者和微生物之间生物相互作用的枢纽,对植物的繁殖和健康有重大影响。以往的研究表明,花朵微生物群在开花期间经历了动态的组装过程。然而,觅食传粉昆虫对花卉微生物群的组装和传播以及植物病原体的传播的影响仍然知之甚少。在这项研究中,我们利用昆虫排除网来研究传粉昆虫在苹果柱头微生物群的集结和火疫病病原体 Erwinia amylovora 的传播中的作用。我们发现,排除传粉昆虫对苹果柱头微生物群落多样性和组成的影响较小,而花的发育阶段则有很大影响。此外,授粉者的排斥改变了细菌的散播和不同细菌种类(包括淀粉样芽孢杆菌)的相对丰度,这表明授粉者在传播植物病原体方面发挥了作用。我们利用报告系统证明,熊蜂能在受控生长条件下从受感染的花朵中传播火疫病病原体。我们的研究强调了固有微生物和不依赖授粉者的微生物作为柱头微生物群接种体来源的重要性,并强调了觅食授粉者在传播植物病原体中的作用。
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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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