Microfluidic Fabrication of Gelatin-Nano Hydroxyapatite Scaffolds for Enhanced Control of Pore Size Distribution and Osteogenic Differentiation of Dental Pulp Stem Cells.

IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Macromolecular bioscience Pub Date : 2024-10-10 DOI:10.1002/mabi.202400279
Cem Bayram, Sukru Ozturk, Beren Karaosmanoglu, Merve Gultekinoglu, Ekim Z Taskiran, Kezban Ulubayram, Hamta Majd, Jubair Ahmed, Mohan Edirisinghe
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Abstract

The combination of gelatin and hydroxyapatite (HA) has emerged as a promising strategy in dental tissue engineering due to its favorable biocompatibility, mechanical properties, and ability to support cellular activities essential for tissue regeneration, rendering them ideal components for hard tissue applications. Besides, precise control over interconnecting porosity is of paramount importance for tissue engineering materials. Conventional methods for creating porous scaffolds frequently encounter difficulties in regulating pore size distribution. This study demonstrates the fabrication of gelatin-nano HA scaffolds with uniform porosity using a T-type junction microfluidic device in a single-step process. Significant improvements in control over the pore size distribution are achieved by regulating the flow parameters, resulting in effective and time-efficient manufacturing comparable in quality to the innovative 3D bioprinting techniques. The overall porosity of the scaffolds exceeded 60%, with a remarkably narrow size distribution. The incorporation of nano-HAinto 3D porous gelatin scaffolds successfully induced osteogenic differentiation in stem cells at both the protein and gene levels, as evidenced by the significant increase in osteocalcin (OCN), an important marker of osteogenic differentiation. The OCN levels are 26 and 43 times higher for gelatin and gelatin-HA scaffolds, respectively, compared to the control group.

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明胶-纳米羟基磷灰石支架的微流控制造,用于加强对孔径分布和牙髓干细胞成骨分化的控制
明胶和羟基磷灰石(HA)具有良好的生物相容性、机械性能和支持组织再生所必需的细胞活动的能力,使它们成为硬组织应用的理想成分,因此明胶和羟基磷灰石(HA)的结合已成为牙科组织工程中一种前景广阔的策略。此外,精确控制相互连接的孔隙率对于组织工程材料来说至关重要。传统的多孔支架制造方法在调节孔径分布方面经常遇到困难。本研究展示了使用 T 型结微流体设备一步法制造具有均匀孔隙率的明胶-纳米 HA 支架。通过调节流动参数,孔径分布的控制得到了显著改善,从而实现了高效、省时的制造,其质量可与创新的三维生物打印技术相媲美。支架的总体孔隙率超过 60%,孔径分布非常窄。在三维多孔明胶支架中加入纳米HA后,成功地在蛋白质和基因水平上诱导了干细胞的成骨分化,成骨分化的重要标志物骨钙素(OCN)的显著增加就是证明。与对照组相比,明胶和明胶-HA支架的OCN水平分别高出26倍和43倍。
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来源期刊
Macromolecular bioscience
Macromolecular bioscience 生物-材料科学:生物材料
CiteScore
7.90
自引率
2.20%
发文量
211
审稿时长
1.5 months
期刊介绍: Macromolecular Bioscience is a leading journal at the intersection of polymer and materials sciences with life science and medicine. With an Impact Factor of 2.895 (2018 Journal Impact Factor, Journal Citation Reports (Clarivate Analytics, 2019)), it is currently ranked among the top biomaterials and polymer journals. Macromolecular Bioscience offers an attractive mixture of high-quality Reviews, Feature Articles, Communications, and Full Papers. With average reviewing times below 30 days, publication times of 2.5 months and listing in all major indices, including Medline, Macromolecular Bioscience is the journal of choice for your best contributions at the intersection of polymer and life sciences.
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