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Surface Coating of ZIF-8 Nanoparticles with Polyacrylic Acid: A Facile Approach to Enhance Chemical Stability for Biomedical Applications. 用聚丙烯酸对 ZIF-8 纳米粒子进行表面涂层:增强生物医学应用中化学稳定性的简便方法。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-11-03 DOI: 10.1002/mabi.202400382
Setsuko Yamane, Abdul Hadi Bin Yusri, Po-Yu Chen, André J van der Vlies, Amira Ben Mabrouk, Isabelle Fetzer, Urara Hasegawa

Nanoparticles of zeolitic imidazole framework-8 (ZIF-8 NPs), which are the subclass of metal-organic frameworks consisting of Zn ion and 2-methylimidazole, have been identified as promising drug carriers since their large microporous structure is suited for encapsulating hydrophobic drug molecules. However, one of the limitations of ZIF-8 NPs is their low stability in physiological solutions, especially in the presence of water and phosphate anions. These molecules can interact with the coordinatively unsaturated Zn sites at the external surface to induce the degradation of ZIF-8 NPs. In this study, herein a facile approach is reported to enhance the chemical stability of ZIF-8 NPs by surface coating with polyacrylic acid (PAA). The PAA-coated ZIF-8 (PAA-ZIF-8) NPs are prepared by mixing ZIF-8 NPs and PAA in water. PAA coating inhibits the degradation of ZIF-8 NPs in water as well as phosphate-buffered saline over 6 days, which seems to be due to the coordination of carboxyl groups of PAA to the reactive Zn sites. Furthermore, the PAA-ZIF-8 NPs loaded with the anticancer drug doxorubicin (Dox) show cytotoxicity in human colon cancer cells. These results clearly show the feasibility of the PAA coating approach to improve the chemical stability of ZIF-8 NPs without impairing their drug delivery capability.

沸石咪唑框架-8(ZIF-8 NPs)纳米粒子是由锌离子和 2-甲基咪唑组成的金属有机框架的亚类,由于其大微孔结构适合封装疏水性药物分子,因此被认为是很有前途的药物载体。然而,ZIF-8 NPs 的局限性之一是其在生理溶液中的稳定性较低,尤其是在有水和磷酸盐阴离子存在的情况下。这些分子会与外表面配位不饱和的锌位点相互作用,导致 ZIF-8 NPs 降解。本研究采用一种简便的方法,通过表面涂覆聚丙烯酸(PAA)来增强 ZIF-8 NPs 的化学稳定性。PAA 涂层 ZIF-8 (PAA-ZIF-8) NPs 由 ZIF-8 NPs 和 PAA 在水中混合制备而成。PAA 涂层可抑制 ZIF-8 NPs 在水中和磷酸盐缓冲盐水中 6 天的降解,这似乎是由于 PAA 的羧基与反应性 Zn 位点配位所致。此外,负载抗癌药物多柔比星(Dox)的 PAA-ZIF-8 NPs 对人类结肠癌细胞具有细胞毒性。这些结果清楚地表明了 PAA 涂层方法在不影响 ZIF-8 NPs 药物输送能力的前提下提高其化学稳定性的可行性。
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引用次数: 0
The Application of Biomaterial-Based Spinal Cord Tissue Engineering. 基于生物材料的脊髓组织工程的应用。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-29 DOI: 10.1002/mabi.202400444
Liang Ma, Zhen Zhang, Yulei Mu, Bangheng Liu, Huiqun Zhou, Dong-An Wang

Advancements in biomaterial-based spinal cord tissue engineering technology have profoundly influenced regenerative medicine, providing innovative solutions for both spinal cord organoid development and engineered spinal cord injury (SCI) repair. In spinal cord organoids, biomaterials offer a supportive microenvironment that mimics the natural extracellular matrix, facilitating cell differentiation and organization and advancing the understanding of spinal cord development and pathophysiology. Furthermore, biomaterials are essential in constructing engineered spinal cords for SCI repair. The incorporation of biomaterials with growth factors, fabrication of ordered scaffold structures, and artificial spinal cord assemblies are critical insights for SCI to ensure structural integrity, enhance cell viability, and promote neural regeneration in transplantation. In summary, this review summarizes the contribution of biomaterials to the spinal cord organoids progression and discusses strategies for biomaterial-based spinal cord engineering in SCI therapy. These achievements underscore the transformative potential of biomaterials to improve treatment options for SCI and accelerate future clinical applications.

基于生物材料的脊髓组织工程技术的进步对再生医学产生了深远的影响,为脊髓类器官的发育和脊髓损伤(SCI)的工程修复提供了创新的解决方案。在脊髓类器官中,生物材料提供了模拟天然细胞外基质的支持性微环境,促进了细胞的分化和组织,增进了对脊髓发育和病理生理学的了解。此外,生物材料对于构建用于 SCI 修复的工程脊髓至关重要。生物材料与生长因子的结合、有序支架结构的制造以及人工脊髓组装是治疗 SCI 的关键见解,可确保结构完整性、提高细胞活力并促进移植中的神经再生。总之,本综述总结了生物材料对脊髓器官组织进展的贡献,并讨论了基于生物材料的脊髓工程在 SCI 治疗中的应用策略。这些成就强调了生物材料在改善 SCI 治疗方案和加速未来临床应用方面的变革潜力。
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引用次数: 0
Biocompatible Zn-Phthalocyanine/Gelatin Nanofiber Membrane for Antibacterial Therapy. 用于抗菌治疗的生物相容性锌-酞菁/明胶纳米纤维膜
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-29 DOI: 10.1002/mabi.202400334
Romina Clementi, Maria Angela Vargas, Mariana Cid, Nancy Salvatierra, Romina Comín, Tomas Tempesti

In this study, the fabrication and characterization of Zn-phthalocyanine/gelatin nanofibrous membranes is reported using the electrospinning technique. The membranes exhibit a homogeneous distribution of Zn-phthalocyanine within the gelatin matrix, maintaining the structural integrity and photosensitizing properties of the phthalocyanine. Scanning electron microscopy revealed that the electrospun fibers possess diameters ranging results as 100-300, 200-700, and 300-800 nm for Gel, ZnPc/Gel 1, and ZnPc/Gel 2, respectively. The addition of ZnPc does not decrease the hydrophilicity of the Gel membrane. The nanofibrous membranes showed good cytocompatibility, as indicated by the high viability of Vero cells exposed to membrane extracts. Furthermore, these composites supported cell adhesion and proliferation on their surfaces. The two Zn-phthalocyanine/gelatin nanofiber formulations exhibited significant antimicrobial activity toward Escherichia Coli (E. Coli) and Staphylococcus Aureus (S. Aureus) under visible light illumination, achieving reductions of 3.4 log10 and 3.6 log10 CFU mL-1 for E. coli, and 3.9 log10 and 4.1 log10 CFU mL-1 for S. aureus. These results demonstrate the potential of Zn-phthalocyanine/gelatin nanofibrous membranes as effective agents in antibacterial photodynamic therapy, providing a promising solution to control bacterial infections and antibiotic resistance.

本研究采用电纺丝技术制备了酞菁锌/明胶纳米纤维膜,并对其进行了表征。这种膜在明胶基质中呈现出酞菁锌的均匀分布,保持了酞菁的结构完整性和光敏特性。扫描电子显微镜显示,凝胶、ZnPc/凝胶 1 和 ZnPc/Gel 2 电纺纤维的直径分别为 100-300、200-700 和 300-800 纳米。添加 ZnPc 不会降低凝胶膜的亲水性。纳米纤维膜具有良好的细胞相容性,暴露在膜提取物中的 Vero 细胞具有很高的存活率。此外,这些复合材料还支持细胞在其表面粘附和增殖。在可见光照射下,两种锌酞菁/明胶纳米纤维配方对大肠杆菌(E. Coli)和金黄色葡萄球菌(S. Aureus)具有显著的抗菌活性,大肠杆菌的抗菌活性分别降低了 3.4 log10 和 3.6 log10 CFU mL-1,金黄色葡萄球菌的抗菌活性分别降低了 3.9 log10 和 4.1 log10 CFU mL-1。这些结果证明了掺酞菁锌/明胶纳米纤维膜作为抗菌光动力疗法有效制剂的潜力,为控制细菌感染和抗生素耐药性提供了一种前景广阔的解决方案。
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引用次数: 0
Applications of Diels-Alder Chemistry in Biomaterials and Drug Delivery. Diels-Alder 化学在生物材料和药物输送中的应用。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-26 DOI: 10.1002/mabi.202400274
Tyus J Yeingst, Angelica M Helton, Daniel J Hayes

Recent studies, leveraging click chemistry reactions, have significantly advanced the fields of biomaterials and drug delivery. Of these click reactions, the Diels-Alder cycloaddition is exceptionally valuable for synthetic organic chemistry and biomaterial design, as it occurs under mild reaction conditions and can undergo a retrograde reaction, under physiologically relevant conditions, to yield the initial reactants. In this review, potential applications of the Diels-Alder reaction are explored within the nexus of biomaterials and drug delivery. This includes an emphasis on key platforms such as polymers, nanoparticles, and hydrogels which utilize Diels-Alder for drug delivery, functionalized surfaces, bioconjugation, and other diverse applications. Specifically, this review will focus on the use of Diels-Alder biomaterials in applications of tissue engineering and cancer therapies, while providing a discussion of the advantages, platforms, and applications of Diels-Alder click chemistry.

最近的研究利用点击化学反应大大推动了生物材料和药物输送领域的发展。在这些点击反应中,Diels-Alder 环加成反应对合成有机化学和生物材料设计具有特别重要的价值,因为它发生在温和的反应条件下,并能在生理相关条件下发生逆反应,生成初始反应物。本综述探讨了 Diels-Alder 反应在生物材料和药物输送领域的潜在应用。其中包括聚合物、纳米颗粒和水凝胶等关键平台,这些平台利用 Diels-Alder 进行药物输送、功能化表面、生物共轭和其他各种应用。具体而言,本综述将重点关注 Diels-Alder 生物材料在组织工程和癌症疗法中的应用,同时讨论 Diels-Alder 点击化学的优势、平台和应用。
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引用次数: 0
Manufacturing Radially Aligned PCL Nanofibers Reinforced With Sulfated Levan and Evaluation of its Biological Activity for Healing Tympanic Membrane Perforations. 用硫酸化莱凡制造径向排列的 PCL 纳米纤维并评估其愈合鼓膜穿孔的生物活性。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-26 DOI: 10.1002/mabi.202400291
Busra Akgul, Cansu Gulcan, Selay Tornaci, Merve Erginer, Ebru Toksoy Oner, Emrah Sefik Abamor, Serap Acar, Adil M Allahverdiyev

The main objective of this study is to construct radially aligned PCL nanofibers reinforced with levan polymer and investigate their in vitro biological activities thoroughly. First Halomonas levan (HL) polysaccharide is hydrolyzed (hHL) and subjected to sulfation to attain Sulfated hydrolyzed Halomonas levan (ShHL)-based material indicating heparin mimetic properties. Then, optimization studies are carried out to produce coaxially generated radially aligned Poly(caprolactone) (PCL) -ShHL nanofibers via electrospinning. The obtained nanofibers are characterized with Fourier Transform Infrared Spectroscopy (FTIR) and Field Emission Scanning Electron Microscopy with Energy Dispersive X-Ray (FESEM-EDX) analysis, and mechanical, contact angle measurement, biodegradability, and swelling tests as well. Afterward, cytotoxicity of artificial tympanic membranes is analyzed by MTT (3-(4,5-Dimethylthiazol-2-yl) -2,5 Diphenyltetrazolium Bromide) test, and their impacts on cell proliferation, cellular adhesion, wound healing processes are explored. Furthermore, an additional FESEM imaging is performed to manifest the interactions between fibroblasts and nanofibers. According to analytical measurements it is detected that PCL-ShHL nanofibers i) are smaller in fiber diameter, ii) are more biodegradable, iii) are more hydrophilic, and iv) demonstrated superior mechanical properties compared to PCL nanofibers. Moreover, it is also deciphered that PCL-ShHL nanofibers strongly elevated cellular adhesion, proliferation, and in vitro wound healing features compared to PCL nanofibers. According to obtained results it is assumed that newly synthetized levan and PCL mediated nanofibers are very encouraging for healing tympanic membrane perforations.

本研究的主要目的是用莱万聚合物构建径向排列的 PCL 纳米纤维,并深入研究其体外生物活性。首先水解 Halomonas levan(HL)多糖(hHL)并进行硫酸化处理,以获得硫酸化水解 Halomonas levan(ShHL)基材料,该材料具有肝素模拟特性。然后进行优化研究,通过电纺丝生产出同轴径向排列的聚己内酯(PCL)-ShHL 纳米纤维。通过傅立叶变换红外光谱(FTIR)和场发射扫描电子显微镜与能量色散 X 射线(FESEM-EDX)分析,以及力学、接触角测量、生物降解性和溶胀测试,对获得的纳米纤维进行了表征。随后,通过 MTT(3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑)测试分析了人工鼓膜的细胞毒性,并探讨了它们对细胞增殖、细胞粘附和伤口愈合过程的影响。此外,还进行了额外的 FESEM 成像,以显示成纤维细胞和纳米纤维之间的相互作用。分析测量结果表明,与 PCL 纳米纤维相比,PCL-ShHL 纳米纤维 i) 纤维直径更小;ii) 生物可降解性更好;iii) 亲水性更强;iv) 机械性能更优越。此外,与 PCL 纳米纤维相比,PCL-ShHL 纳米纤维还能显著提高细胞粘附性、增殖性和体外伤口愈合功能。根据所获得的结果,可以认为新合成的 Levan 和 PCL 介导的纳米纤维对鼓膜穿孔的愈合非常有帮助。
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引用次数: 0
Exploring Morphological and Molecular Properties of Different Adipose Cell Models: Monolayer, Spheroids, Gellan Gum-Based Hydrogels, and Explants. 探索不同脂肪细胞模型的形态和分子特性:单层、球形、结冷胶水凝胶和外植体
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-25 DOI: 10.1002/mabi.202400320
Franziska B Albrecht, Ann-Kathrin Schick, Annemarie Klatt, Freia F Schmidt, Svenja Nellinger, Petra J Kluger

White adipose tissue (WAT) plays a crucial role in energy homeostasis and secretes numerous adipokines with far-reaching effects. WAT is linked to diseases such as diabetes, cardiovascular disease, and cancer. There is a high demand for suitable in vitro models to study diseases and tissue metabolism. Most of these models are covered by 2D-monolayer cultures. This study aims to evaluate the performance of different WAT models to better derive potential applications. The stability of adipocyte characteristics in spheroids and two 3D gellan gum hydrogels with ex situ lobules and 2D-monolayer culture is analyzed. First, the differentiation to achieve adipocyte-like characteristics is determined. Second, to evaluate the maintenance of differentiated ASC-based models, an adipocyte-based model, and explants over 3 weeks, viability, intracellular lipid content, perilipin A expression, adipokine, and gene expression are analyzed. Several advantages are supported using each of the models. Including, but not limited to, the strong differentiation in 2D-monolayers, the self-assembling within spheroids, the long-term stability of the stem cell-containing hydrogels, and the mature phenotype within adipocyte-containing hydrogels and the lobules. This study highlights the advantages of 3D models due to their more in vivo-like behavior and provides an overview of the different adipose cell models.

白色脂肪组织(WAT)在能量平衡中起着至关重要的作用,并能分泌多种具有深远影响的脂肪因子。白脂肪组织与糖尿病、心血管疾病和癌症等疾病有关。研究疾病和组织代谢需要大量合适的体外模型。这些模型大多采用二维单层培养。本研究旨在评估不同 WAT 模型的性能,以更好地推导其潜在应用。研究分析了球形和两种三维结冷胶水凝胶与原位小叶和二维单层培养中脂肪细胞特性的稳定性。首先,确定分化以获得类似脂肪细胞的特征。其次,为了评估已分化的基于 ASC 的模型、基于脂肪细胞的模型和外植体在 3 周内的维持情况,对活力、细胞内脂质含量、过脂素 A 表达、脂肪因子和基因表达进行了分析。每种模型都有若干优点。包括但不限于二维单层中的强分化、球体内的自组装、含干细胞水凝胶的长期稳定性以及含脂肪细胞水凝胶和小叶内的成熟表型。这项研究强调了三维模型的优势,因为它们更像活体行为,并概述了不同的脂肪细胞模型。
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引用次数: 0
Viscosity Characteristics of Ideal Lubricants to Reduce Stress on Intestinal Mucosa During Balloon-Assisted Enteroscopy. 理想润滑剂的粘度特性,以减轻球囊辅助肠镜检查过程中对肠道粘膜的压力。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-22 DOI: 10.1002/mabi.202400285
Katsuma Yamauchi, Ryohei Hirose, Hiroshi Ikegaya, Hiroki Mukai, Hajime Miyazaki, Naoto Watanabe, Takuma Yoshida, Risa Bandou, Ken Inoue, Osamu Dohi, Naohisa Yoshida, Takaaki Nakaya, Yoshito Itoh

Balloon-assisted enteroscopy (BAE) is highly invasive and carries a higher risk of complications such as pain and perforation during enteroscope insertion. Applying lubricants to the small intestinal mucosa and reducing the dynamic friction coefficient (DFC) between the small intestinal mucosa and endoscopic shaft (ES) (or overtube (OT)) can minimize the invasiveness of BAE. However, the ideal viscosity characteristics of these lubricants remain unknown. In this study, a model is developed to measure the DFC using human small intestines from forensic autopsies and determine the ideal viscosity of low-friction lubricants that exhibit a minimal DFC, thus reducing the pressure on the intestinal lining during the procedure. The results reveal that the DFC is strongly correlated to the lubricant's viscosity rather than its chemical composition. Low-friction lubricants with viscosities within 0.20-0.32 and 0.35-0.58 Pa·s (at shear rates of 10 s-1) for the OT and ES, respectively, can significantly reduce the DFC, yielding optimal results. These findings highlight the role of viscosity in minimizing the friction between the equipment and small intestinal mucosa. The ideal low-friction lubricants satisfying the aforementioned viscosity ranges can minimize the invasiveness of BAE by decreasing the friction between the equipment and intestinal lining.

球囊辅助肠镜(BAE)具有高度侵入性,在插入肠镜时发生疼痛和穿孔等并发症的风险较高。在小肠粘膜上涂抹润滑剂并降低小肠粘膜与内镜轴 (ES) (或过管 (OT))之间的动态摩擦系数 (DFC),可将 BAE 的侵入性降至最低。然而,这些润滑剂的理想粘度特性仍然未知。本研究开发了一个模型,利用法医解剖的人体小肠来测量 DFC,并确定低摩擦润滑剂的理想粘度,以显示最小的 DFC,从而减少手术过程中对肠道内壁的压力。结果显示,DFC 与润滑剂的粘度而非化学成分密切相关。OT和ES的低摩擦润滑剂粘度分别在0.20-0.32 Pa-s和0.35-0.58 Pa-s(剪切速率为10 s-1)范围内,可显著降低DFC,达到最佳效果。这些发现凸显了粘度在最大程度减少设备与小肠粘膜之间摩擦方面的作用。符合上述粘度范围的理想低摩擦润滑剂可以通过减少设备与肠粘膜之间的摩擦,最大程度地降低 BAE 的侵袭性。
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引用次数: 0
A Facile Strategy for Preparing Flexible and Porous Hydrogel-Based Scaffolds from Silk Sericin/Wool Keratin by In Situ Bubble-Forming for Muscle Tissue Engineering Applications. 利用原位气泡成形法制备柔性多孔水凝胶支架的简便策略,用于肌肉组织工程应用
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-20 DOI: 10.1002/mabi.202400362
Elif Beyza Demiray, Tugba Sezgin Arslan, Burak Derkus, Yavuz Emre Arslan

In the present study, it is aimed to fabricate a novel silk sericin (SS)/wool keratin (WK) hydrogel-based scaffolds using an in situ bubble-forming strategy containing an N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) coupling reaction. During the rapid gelation process, CO2 bubbles are released by activating the carboxyl groups in sericin with EDC and NHS, entrapped within the gel, creating a porous cross-linked structure. With this approach, five different hydrogels (S2K1, S4K2, S2K4, S6K3, and S3K6) are constructed to investigate the impact of varying sericin and keratin ratios. Analyses reveal that more sericin in the proteinaceous mixture reinforced the hydrogel network. Additionally, the hydrogels' pore size distribution, swelling ratio, wettability, and in vitro biodegradation rate, which are crucial for the applications of biomaterials, are evaluated. Moreover, biocompatibility and proangiogenic properties are analyzed using an in-ovo chorioallantoic membrane assay. The findings suggest that the S4K2 hydrogel exhibited the most promising characteristics, featuring an adequately flexible and highly porous structure. The results obtained by in vitro assessments demonstrate the potential of S4K2 hydrogel in muscle tissue engineering. However, further work is necessary to improve hydrogels with an aligned structure to meet the features that can fully replace muscle tissue for volumetric muscle loss regeneration.

本研究旨在采用原位气泡形成策略(包含 N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) 和 N-hydroxysuccinimide (NHS) 偶联反应),制造新型丝胶/羊毛角蛋白水凝胶支架。在快速凝胶化过程中,丝胶蛋白中的羧基被 EDC 和 NHS 激活,释放出二氧化碳气泡,夹杂在凝胶中,形成多孔交联结构。利用这种方法构建了五种不同的水凝胶(S2K1、S4K2、S2K4、S6K3 和 S3K6),以研究不同丝胶素和角蛋白比例的影响。分析表明,蛋白混合物中丝胶含量越高,水凝胶网络越强。此外,还对水凝胶的孔径分布、溶胀率、润湿性和体外生物降解率进行了评估,这些因素对生物材料的应用至关重要。此外,研究人员还使用绒毛膜试验分析了水凝胶的生物相容性和促血管生成特性。研究结果表明,S4K2 水凝胶表现出最有前途的特性,具有足够的柔韧性和高多孔结构。体外评估的结果证明了 S4K2 水凝胶在肌肉组织工程中的潜力。不过,还需要进一步改进水凝胶的排列结构,以满足可完全替代肌肉组织进行肌肉体积再生的特性。
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引用次数: 0
Silk Composite-Based Multifunctional Pellets for Controlled Release. 基于蚕丝复合材料的多功能控释颗粒。
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-20 DOI: 10.1002/mabi.202400410
Sushma Indrakumar, Sriram Bharath Gugulothu, Akshat Joshi, Tapan Kumar Dash, Vivek Mishra, Bharat Tandon, Kaushik Chatterjee

Chronic wounds present significant clinical challenges due to the high risk of infections and persistent inflammation. While personalized treatments in point-of-care settings are crucial, they are limited by the complex fabrication techniques of the existing products. The calcium sulfate hemihydrate (CSH)-based drug delivery platform enables rapid fabrication but lacks antioxidant and antibacterial properties, essential to promote healing. To develop a multifunctional platform, a tannic acid (TA)-silk fibroin (SF) complex is engineered and incorporated as an additive in CSH cement. This cement is then cast into pellets to create silk/bioceramic-based composite drug delivery systems, designed for point-of-care use. Compared to neat CSH pellets, the composite pellets exhibit a 7.5-fold increase in antioxidant activity and prolonged antibacterial efficacy (up to 13 d). Moreover, the subcutaneous implantation of the pellets shows no hallmarks of local or systemic toxicity in a rodent model. The pellets are optimized in composition and fabrication to ease market translation. Clinically, the pellets have the potential to be further developed into products to place on wound beds or fill into bone cavities that are designed to deliver the intended therapeutic effect. The developed multifunctional system proves to be a promising solution for personalized treatment in point-of-care settings.

由于感染和持续炎症的风险很高,慢性伤口给临床带来了巨大挑战。虽然护理点的个性化治疗至关重要,但现有产品复杂的制造技术限制了这种治疗。基于半水硫酸钙(CSH)的给药平台可以快速制造,但缺乏促进伤口愈合所必需的抗氧化和抗菌特性。为了开发多功能平台,我们设计了一种单宁酸(TA)-丝状纤维蛋白(SF)复合物,并将其作为添加剂加入半水硫酸钙水泥中。然后将这种水泥浇铸成颗粒,制成丝/生物陶瓷基复合给药系统,专供护理点使用。与纯 CSH 颗粒相比,复合颗粒的抗氧化活性提高了 7.5 倍,抗菌效力延长(长达 13 天)。此外,在啮齿动物模型中,皮下植入颗粒没有显示出局部或全身毒性。颗粒的成分和制造工艺均已优化,便于市场转化。在临床上,这种颗粒有可能进一步开发成产品,用于伤口床或填充骨腔,以达到预期的治疗效果。事实证明,所开发的多功能系统是在护理点环境下进行个性化治疗的一种很有前途的解决方案。
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引用次数: 0
Tunable Blended Collagen I/II and Collagen I/III Hydrogels as Tissue Mimics. 作为组织模拟物的可调混合胶原 I/II 和胶原 I/III 水凝胶
IF 4.4 4区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Pub Date : 2024-10-20 DOI: 10.1002/mabi.202400280
Paulina M Babiak, Carly M Battistoni, Leonard Cahya, Rithika Athreya, Jason Minnich, Alyssa Panitch, Julie C Liu

Collagen (Col) is commonly used as a natural biomaterial for biomedical applications. Although Col I is the most prevalent col type employed, many collagen types work together in vivo to confer function and biological activity. Thus, blending collagen types can better recapitulate many native environments. This work investigates how hydrogel properties can be tuned through blending collagen types (col I/II and col I/III) and by varying polymerization temperatures. Col I/II results in poorly developed fibril networks, which softened the gels, especially at lower polymerization temperatures. Conversely, col I/III hydrogels exhibit well-connected fibril networks with localized areas of fine fibrils and result in stiffer hydrogels. A decreased molecular mass recovery rate is observed in blended hydrogels. The altered fibril morphologies, mechanical properties, and biological signals of the blended gels can be leveraged to alter cell responses and can be used as models for different tissue types (e.g., healthy vs fibrotic tissue). Furthermore, the biomimetic hydrogel properties are a tool that can be used to modulate the transport of drugs, nutrients, and wastes in tissue engineering applications.

胶原蛋白(Col)是生物医学应用中常用的天然生物材料。虽然Ⅰ型胶原蛋白是最常用的胶原蛋白类型,但许多胶原蛋白类型在体内共同作用,赋予人体功能和生物活性。因此,混合胶原类型可以更好地再现许多原生环境。这项工作研究了如何通过混合胶原类型(胶原 I/II 和胶原 I/III)和改变聚合温度来调整水凝胶的特性。Col I/II 导致纤维网络发育不良,从而软化了凝胶,尤其是在较低的聚合温度下。相反,Col I/III 水凝胶显示出连接良好的纤维网络,局部区域有细纤维,因此水凝胶较硬。在混合水凝胶中观察到分子质量恢复率降低。混合凝胶改变的纤维形态、机械性能和生物信号可用于改变细胞反应,并可用作不同组织类型(如健康组织与纤维化组织)的模型。此外,仿生水凝胶的特性还是一种工具,可用于调节组织工程应用中药物、营养物质和废物的运输。
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引用次数: 0
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Macromolecular bioscience
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