GhMAC3e is involved in plant growth and defense response to Verticillium dahliae.

IF 5.3 2区 生物学 Q1 PLANT SCIENCES Plant Cell Reports Pub Date : 2024-10-10 DOI:10.1007/s00299-024-03348-y
Zhenghong Han, Yuanyuan Qiu, Ting Pan, Longjie Wang, Jing Wang, Kang Liu
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Abstract

Key message: GhMAC3e expression was induced by various stresses and hormones. GhMAC3e may regulate plant growth by influencing auxin distribution, and play important roles in Verticillium wilt resistance via mediating SA signaling. The MOS4-Associated Complex (MAC) is a highly conserved protein complex involved in pre-mRNA splicing and spliceosome assembly, which plays a vital role in plant immunity. It comprises key components such as MOS4, CDC5, and PRL1. MAC3A/B, as U-box E3 ubiquitin ligases, are crucial for various plant processes including development, stress responses, and disease resistance. However, their roles in cotton remain largely unknown. In this study, we first cloned the GhMAC3e gene from cotton and explored its biological functions by using virus-induced gene silencing (VIGS) in cotton and transgenic overexpression in Arabidopsis. The results showed that GhMAC3e is ubiquitously expressed in cotton tissues and could be induced by salt stress, Verticillium dahliae (VD) infection, PEG, ABA, ETH, GA3, MeJA, and SA. Silencing GhMAC3e retarded primary stem growth and reduced biomass of cotton coupled with the reduced auxin content in the petioles and veins. Silencing GhMAC3e up-regulated expression of cell growth-related genes GhXTH16 and Gh3.6, while down-regulated GhSAUR12 expression. Ectopic expression of GhMAC3e in Arabidopsis significantly enhanced its resistance to Verticillium wilt (VW) in terms of decreased pathogen biomass and lowered plant mortality. Overexpression of GhMAC3e dramatically upregulated AtPR1 by around 15 fold and more than 262 fold under basal and VD inoculation condition, respectively. This change was not associated with the expression of GhNPR1. In conclusion, GhMAC3e may not only regulate plant growth by influencing auxin distribution and growth-related gene expression, but also play important roles in VW resistance via mediating SA signaling independent of NPR1 transcription level.

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GhMAC3e 参与植物生长和对大丽轮枝菌的防御反应。
关键信息GhMAC3e的表达受到各种胁迫和激素的诱导。GhMAC3e可能通过影响植物生长素的分布来调控植物生长,并通过介导SA信号转导在轮纹枯萎病抗性中发挥重要作用。MOS4 相关复合物(MAC)是一种高度保守的蛋白质复合物,参与前 mRNA 剪接和剪接体组装,在植物免疫中发挥着重要作用。它由 MOS4、CDC5 和 PRL1 等关键成分组成。MAC3A/B 作为 U-box E3 泛素连接酶,对植物的各种过程(包括发育、胁迫反应和抗病性)至关重要。然而,它们在棉花中的作用在很大程度上仍然未知。在本研究中,我们首先克隆了棉花中的 GhMAC3e 基因,并利用病毒诱导的棉花基因沉默(VIGS)和拟南芥的转基因过表达来探索其生物学功能。结果表明,GhMAC3e 在棉花组织中普遍表达,并可被盐胁迫、大丽轮枝菌(VD)感染、PEG、ABA、ETH、GA3、MeJA 和 SA 诱导。沉默 GhMAC3e 会延缓棉花主茎的生长并降低其生物量,同时叶柄和叶脉中的辅助素含量也会降低。沉默 GhMAC3e 会上调细胞生长相关基因 GhXTH16 和 Gh3.6 的表达,同时下调 GhSAUR12 的表达。在拟南芥中异位表达 GhMAC3e 能显著增强其对轮纹枯萎病(VW)的抗性,表现为病原体生物量的减少和植株死亡率的降低。在基础和VD接种条件下,GhMAC3e的过表达分别显著上调了AtPR1约15倍和超过262倍。这种变化与 GhNPR1 的表达无关。总之,GhMAC3e不仅可能通过影响植物生长素的分布和生长相关基因的表达来调控植物的生长,而且还可能通过介导SA信号转导(与NPR1转录水平无关)在植物的抗逆性中发挥重要作用。
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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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